Key words : in vitro rooting, enzyme immunoassay (ELISA), abscisic acid (ABA), indole-3acetic acid (IAA), indole-3-butyric acid (IBA), Prunus avium L.Abstract. Levels of endogenous ABA and IAA were quantified during the first week of in vitro rooting of Wild Cherry (Prunus avium L .) using IBA in the culture medium . Hormones were measured in the apical, median and basal parts of the explants using an avidin-biotin based enzyme linked immunosorbent assay (ELISA), after a purification of the methanolic extracts by high-performance liquid chromatography (HPLC) .Root primordia started to differentiate from day 5 at the basal part of the explants . ABA and IAA showed considerable changes and high levels were detected during the first week of culture . ABA levels increased transiently mainly in the apical part during root formation . Exogenous IBA was possibly transformed into IAA mainly in the basal part of the explants .