Endogenous musculoskeletal tissue engineering - a focused perspective

Adam, Clayton J.

doi:10.1007/s00441-011-1234-2

Cited by 17 publications

(8 citation statements)

References 85 publications

(95 reference statements)

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“…19,20 These results on vascular homeostasis are significant for revealing the mechanism of mechanical control of vascular growth, regeneration, and remodeling in vivo. 21 More indepth reviews on mechanically driven homeostasis are available for cardiac, 22,23 vasculature, 20,24 bone, 25 muscle, 26 and tendon cells. 27 …”

Section: Mechanical Cue and Homeostasismentioning

confidence: 99%

Mechanical Stretching for Tissue Engineering: Two-Dimensional and Three-Dimensional Constructs

Riehl

Park

Kwon

et al. 2012

Tissue Engineering Part B: Reviews

176

175

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Mechanical cell stretching may be an attractive strategy for the tissue engineering of mechanically functional tissues. It has been demonstrated that cell growth and differentiation can be guided by cell stretch with minimal help from soluble factors and engineered tissues that are mechanically stretched in bioreactors may have superior organization, functionality, and strength compared with unstretched counterparts. This review explores recent studies on cell stretching in both two-dimensional (2D) and three-dimensional (3D) setups focusing on the applications of stretch stimulation as a tool for controlling cell orientation, growth, gene expression, lineage commitment, and differentiation and for achieving successful tissue engineering of mechanically functional tissues, including cardiac, muscle, vasculature, ligament, tendon, bone, and so on. Custom stretching devices and lab-specific mechanical bioreactors are described with a discussion on capabilities and limitations. While stretch mechanotransduction pathways have been examined using 2D stretch, studying such pathways in physiologically relevant 3D environments may be required to understand how cells direct tissue development under stretch. Cell stretch study using 3D milieus may also help to develop tissue-specific stretch regimens optimized with biochemical feedback, which once developed will provide optimal tissue engineering protocols.

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Section: Mechanical Cue and Homeostasismentioning

confidence: 99%

Mechanical Stretching for Tissue Engineering: Two-Dimensional and Three-Dimensional Constructs

Riehl

Park

Kwon

et al. 2012

Tissue Engineering Part B: Reviews

176

175

View full text Add to dashboard Cite

show abstract

“…In addition to immune considerations, this concept overcomes the difficulties and expense associated with cultivating, storing and distributing cells outside the human body. This strategy enables the human body to be used as a “bioreactor” to regenerate damaged tissue by applying a biomaterials‐based approach that does not require the delivery of ex vivo‐expanded cellular materials . A wide range of in vitro and in situ tissue engineering approaches have been pursued for more than three decades, and there have been remarkable discoveries and indisputable success stories from the laboratory to the patient .…”

Section: Introductionmentioning

confidence: 99%

Engineering a Cell Home for Stem Cell Homing and Accommodation

Yin

et al. 2017

Adv. Biosys.

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Distilling complexity to advance regenerative medicine from laboratory animals to humans, in situ regeneration will continue to evolve using biomaterial strategies to drive endogenous cells within the human body for therapeutic purposes; this approach avoids the need for delivering ex vivo‐expanded cellular materials. Ensuring the recruitment of a significant number of reparative cells from an endogenous source to the site of interest is the first step toward achieving success. Subsequently, making the “cell home” cell‐friendly by recapitulating the natural extracellular matrix (ECM) in terms of its chemistry, structure, dynamics, and function, and targeting specific aspects of the native stem cell niche (e.g., cell–ECM and cell–cell interactions) to program and steer the fates of those recruited stem cells play equally crucial roles in yielding a therapeutically regenerative solution. This review addresses the key aspects of material‐guided cell homing and the engineering of novel biomaterials with desirable ECM composition, surface topography, biochemistry, and mechanical properties that can present both biochemical and physical cues required for in situ tissue regeneration. This growing body of knowledge will likely become a design basis for the development of regenerative biomaterials for, but not limited to, future in situ tissue engineering and regeneration.

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“…[1] Due to the limitations associated with conventional approaches such as autografts and allografts, tissue engineering strategies inspired by endogenous bone healing mechanisms have recently attracted more attention. [2-5] Osteogenesis and angiogenesis are considered to be equally important to a tissue engineering bone regeneration strategy since bone is a highly vascularized and mineralized tissue. [6,7] Insufficient angiogenesis during bone regeneration results in poor and unsustainable bone formation.…”

Section: Introductionmentioning

confidence: 99%

Multilayered Inorganic Microparticles for Tunable Dual Growth Factor Delivery

Khalil

Dang

et al. 2014

Adv Funct Materials

118

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There is an increasing need to control the type, quantity, and timing of growth factors released during tissue healing. Sophisticated delivery systems offering the ability to deliver multiple growth factors with independently tunable kinetics are highly desirable. Here, a multilayered, mineral coated micro-particle (MCMs) platform that can serve as an adaptable dual growth factor delivery system is developed. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) are bound to the mineral coatings with high binding efficiencies of up to 80%. BMP-2 is firstly bound onto a 1st mineral coating layer; then VEGF is bound onto a 2nd mineral coating layer. The release of BMP-2 is sustained over a period of 50 days while the release of VEGF is a typical two-phase release with rapid release in the first 14 days and more sustained release for the following 36 days. Notably, the release behaviors of both growth factors can be independently tailored by changing the intrinsic properties of the mineral coatings. Furthermore, the release of BMP-2 can be tuned by changing the thickness of the 2nd layer. This injectable microparticle based delivery platform with tunable growth factor release has immense potential for applications in tissue engineering and regenerative medicine.

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Endogenous musculoskeletal tissue engineering - a focused perspective

Cited by 17 publications

References 85 publications

Mechanical Stretching for Tissue Engineering: Two-Dimensional and Three-Dimensional Constructs

Mechanical Stretching for Tissue Engineering: Two-Dimensional and Three-Dimensional Constructs

Engineering a Cell Home for Stem Cell Homing and Accommodation

Multilayered Inorganic Microparticles for Tunable Dual Growth Factor Delivery

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