Endogenous Optical Signals Reveal Changes of Elastin and Collagen Organization During Differentiation of Mouse Embryonic Stem Cells

Thimm, Terra; Squirrell, Jayne M.; Liu, Yuming; Eliceiri, Kevin W.; Ogle, Brenda M.

doi:10.1089/ten.tec.2014.0699

Cited by 8 publications

(9 citation statements)

References 91 publications

(93 reference statements)

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“…Specifically, in native myocardium, heart compliance and cardiomyocyte alignment and differentiation are strictly dependent on the elastic properties of the tissue. In particular, the content of elastin, the dominant mammalian elastic protein and the main component of elastic fibers (Kielty et al, 2002;Li et al, 2014), has been related to cardiac myocyte differentiation and maturation as it progressively increases in the developing mammalian heart, but then decreases soon after birth (Hanson et al, 2013;Thimm et al, 2015). Interestingly, elastin-based biomaterials have been developed and used to enhance tissue biocompatibility and promote stem cell differentiation (Celebi et al, 2012;Ozsvar et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Belviso

Romano

Sacco

et al. 2020

Front. Bioeng. Biotechnol.

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The complex and highly organized environment in which cells reside consists primarily of the extracellular matrix (ECM) that delivers biological signals and physical stimuli to resident cells. In the native myocardium, the ECM contributes to both heart compliance and cardiomyocyte maturation and function. Thus, myocardium regeneration cannot be accomplished if cardiac ECM is not restored. We hypothesize that decellularized human skin might make an easily accessible and viable alternate biological scaffold for cardiac tissue engineering (CTE). To test our hypothesis, we decellularized specimens of both human skin and human myocardium and analyzed and compared their composition by histological methods and quantitative assays. Decellularized dermal matrix was then cut into 600-µm-thick sections and either tested by uniaxial tensile stretching to characterize its mechanical behavior or used as three-dimensional scaffold to assess its capability to support regeneration by resident cardiac progenitor cells (hCPCs) in vitro. Histological and quantitative analyses of the dermal matrix provided evidence of both effective decellularization with preserved tissue architecture and retention of ECM proteins and growth factors typical of cardiac matrix. Further, the elastic modulus of the dermal matrix resulted comparable with that reported in literature for the human myocardium and, when tested in vitro, dermal matrix resulted a comfortable and protective substrate promoting and supporting hCPC engraftment, survival and cardiomyogenic potential. Our study provides compelling evidence that dermal matrix holds promise as a fully autologous and cost-effective biological scaffold for CTE.

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Section: Introductionmentioning

confidence: 99%

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Belviso

Romano

Sacco

et al. 2020

Front. Bioeng. Biotechnol.

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“…High-throughput analysis during hPSC differentiation showed that genes related to the ECM are modulated throughout the cardiac commitment process (den Hartogh et al, 2016; Pereira et al, 2019). Additionally, it was verified that during CM differentiation, total proteoglycan and hyaluronan decreased (Chan et al, 2010); meanwhile, using an endogenous optical signal, it was showed that elastin increased until day 9 of the murine EB differentiation protocol, and type I collagen (Col I) increased over 12 days (Thimm et al, 2015). Using a spontaneous differentiation protocol and isolating the beating areas, it was demonstrated that hESC-CM were surrounded by types I, IV, and XVIII collagens, laminin isoforms and fibronectin (FN) (van Laake et al, 2010).…”

Section: Exploring the Cardiac Ecm: Composition Tissue Engineering Smentioning

confidence: 95%

Cardiomyogenesis Modeling Using Pluripotent Stem Cells: The Role of Microenvironmental Signaling

Leitolis

Robert

Pereira

et al. 2019

Front. Cell Dev. Biol.

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Pluripotent stem cells (PSC) can be used as a model to study cardiomyogenic differentiation. In vitro modeling can reproduce cardiac development through modulation of some key signaling pathways. Therefore, many studies make use of this strategy to better understand cardiomyogenesis complexity and to determine possible ways to modulate cell fate. However, challenges remain regarding efficiency of differentiation protocols, cardiomyocyte (CM) maturation and therapeutic applications. Considering that the extracellular milieu is crucial for cellular behavior control, cardiac niche studies, such as those identifying secreted molecules from adult or neonatal tissues, allow the identification of extracellular factors that may contribute to CM differentiation and maturation. This review will focus on cardiomyogenesis modeling using PSC and the elements involved in cardiac microenvironmental signaling (the secretome – extracellular vesicles, extracellular matrix and soluble factors) that may contribute to CM specification and maturation.

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“…panel 2.7a) and is spatially associated with cells exhibiting markers of cardiomyocyte maturity (Fig. panel 2.7b) [69]. …”

Section: Cellular–scale Imagingmentioning

confidence: 99%

“…Panel ( a ) Endogenous elastin fluorescence over time. Panel ( b ) Spatial relationship between elastin, collagen, and cardiomyocytes in embryoid bodies [69]. (Copyright granted as paper coauthor (Eliceiri))…”

Section: Fig 21mentioning

confidence: 99%

Imaging the Cardiac Extracellular Matrix

Pinkert

Hortensius

Ogle

et al. 2018

Advances in Experimental Medicine and Biology

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Cardiovascular disease is the global leading cause of death. One route to address this problem is using biomedical imaging to measure the molecules and structures that surround cardiac cells. This cellular microenvironment, known as the cardiac extracellular matrix, changes in composition and organization during most cardiac diseases and in response to many cardiac treatments. Measuring these changes with biomedical imaging can aid in understanding, diagnosing, and treating heart disease. This chapter supports those efforts by reviewing representative methods for imaging the cardiac extracellular matrix. It first describes the major biological targets of ECM imaging, including the primary imaging target of fibrillar collagen. Then it discusses the imaging methods, describing their current capabilities and limitations. It categorizes the imaging methods into two main categories: organ-scale noninvasive methods and cellular-scale invasive methods. Noninvasive methods can be used on patients, but only a few are clinically available, and others require further development to be used in the clinic. Invasive methods are the most established and can measure a variety of properties, but they cannot be used on live patients. Finally, the chapter concludes with a perspective on future directions and applications of biomedical imaging technologies.

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Endogenous Optical Signals Reveal Changes of Elastin and Collagen Organization During Differentiation of Mouse Embryonic Stem Cells

Cited by 8 publications

References 91 publications

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Cardiomyogenesis Modeling Using Pluripotent Stem Cells: The Role of Microenvironmental Signaling

Imaging the Cardiac Extracellular Matrix

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