Endometrial expression of selected genes in patients achieving pregnancy spontaneously or after ICSI and patients failing at least two ICSI cycles

Allegra, Adolfo; Marino, Angelo; Peregrín, Pedro Caballero; Lama, A.; García-Segovia, Áurea; Forte, Giusi Irma; Núñez-Calonge, Rocío; Agueli, Cecilia; Mazzola, Sergio; Volpes, Aldo

doi:10.1016/j.rbmo.2012.07.019

Cited by 10 publications

(6 citation statements)

References 70 publications

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“…52 genes are up-regulated in mid-secretory endometrium, while five are down-regulated (↓). ENTREZ ID HUGO Symbol HUGO Name VAL* VAL** RRA score Adjusted P 5047 PAEP b,c,d,e Progestagen-associated endometrial protein 90 7.68E-18 2.99E-13 6696 SPP1 b,d,e Secreted phosphoprotein 1 (osteopontin) 30 , 32 , 87 , 89 , 91 92 2.06E-15 8.04E-11 2878 GPX3 b,c,d,e Glutathione peroxidase 3 30 , 86 , 88 , 91 1.89E-14 7.40E-10 4128 MAOA a,b,d,e Monoamine oxidase A 17 93 + 2.32E-13 9.04E-09 1647 GADD45A b,c,d,e Growth arrest and DNA-damage-inducible, alpha 2.73E-13 1.06E-08 22943 DKK1 a,b,d,e Dickkopf WNT signalling pathway inhibitor 1 17 , 33 , 85 94 2.80E-13 1.09E-08 1364 CLDN4 a,b,d,e Claudin 4 87 , 88 87 , 95 9.14E-13 3.57E-08 722 C...…”

Section: Resultsmentioning

confidence: 99%

Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers

Altmäe

Koel

Võsa

et al. 2017

Sci Rep

205

180

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Previous transcriptome studies of the human endometrium have revealed hundreds of simultaneously up- and down-regulated genes that are involved in endometrial receptivity. However, the overlap between the studies is relatively small, and we are still searching for potential diagnostic biomarkers. Here we perform a meta-analysis of endometrial-receptivity associated genes on 164 endometrial samples (76 from ‘pre-receptive’ and 88 from mid-secretory, ‘receptive’ phase endometria) using a robust rank aggregation (RRA) method, followed by enrichment analysis, and regulatory microRNA prediction. We identify a meta-signature of endometrial receptivity involving 57 mRNA genes as putative receptivity markers, where 39 of these we confirm experimentally using RNA-sequencing method in two separate datasets. The meta-signature genes highlight the importance of immune responses, the complement cascade pathway and the involvement of exosomes in mid-secretory endometrial functions. Bioinformatic prediction identifies 348 microRNAs that could regulate 30 endometrial-receptivity associated genes, and we confirm experimentally the decreased expression of 19 microRNAs with 11 corresponding up-regulated meta-signature genes in our validation experiments. The 57 identified meta-signature genes and involved pathways, together with their regulatory microRNAs could serve as promising and sought-after biomarkers of endometrial receptivity, fertility and infertility.

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Section: Resultsmentioning

confidence: 99%

Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers

Altmäe

Koel

Võsa

et al. 2017

Sci Rep

205

180

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“…Not much is known about the roles of KRTAP17-1 , RANBP3L , MPP7 , HCP5 and PTPRN2 in endometrial biology. However, CASP8 has been shown to be among the genes dysregulated in women with chronic endometritis and impaired receptivity 19 , and IVF treatment failure 20 , while WT1 is associated with decidualization in rat endometrial stromal cells 21 , and is downregulated during WOI in polycystic ovary syndrome patients 22 . These lines of evidence support their role among the genes modifying the microenvironment within the receptive endometrium.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation changes in endometrium and correlation with gene expression during the transition from pre-receptive to receptive phase

Kukushkina

Modhukur

Suhorutšenko

et al. 2017

Sci Rep

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The inner uterine lining (endometrium) is a unique tissue going through remarkable changes each menstrual cycle. Endometrium has its characteristic DNA methylation profile, although not much is known about the endometrial methylome changes throughout the menstrual cycle. The impact of methylome changes on gene expression and thereby on the function of the tissue, including establishing receptivity to implanting embryo, is also unclear. Therefore, this study used genome-wide technologies to characterize the methylome and the correlation between DNA methylation and gene expression in endometrial biopsies collected from 17 healthy fertile-aged women from pre-receptive and receptive phase within one menstrual cycle. Our study showed that the overall methylome remains relatively stable during this stage of the menstrual cycle, with small-scale changes affecting 5% of the studied CpG sites (22,272 out of studied 437,022 CpGs, FDR < 0.05). Of differentially methylated CpG sites with the largest absolute changes in methylation level, approximately 30% correlated with gene expression measured by RNA sequencing, with negative correlations being more common in 5′ UTR and positive correlations in the gene ‘Body’ region. According to our results, extracellular matrix organization and immune response are the pathways most affected by methylation changes during the transition from pre-receptive to receptive phase.

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“…Reference Collection 30 (Curry et al, 1977;Albers et al, 1981;Cheung et al, 1993;Baker et al, 1994;Vijayaraghavan et al, 1994;Hansen et al, 2004;Iqbal et al, 2008;Navarro et al, 2010a;Edlow et al, 2014;Manjunatha et al, 2016;Ravnsborg et al, 2016;Brahimaj et al, 2017;Herzig et al, 2017;Desmarais et al, 2018;Saadane et al, 2019;Kopylov et al, 2020;Santana et al, 2020). Reference Collection 31 (Bujalska et al, 2006;Appari et al, 2009;Chen et al, 2011;Allegra et al, 2012;Ammerpohl et al, 2013;Zhu et al, 2015;Salami et al, 2019;Tanase-Nakao et al, 2019;Geng et al, 2020). Reference Collection 32 (Albers et al, 1984;Haffner et al, 1985;Holmquist et al, 1993;Gottsch et al, 2003;Zenkel et al, 2005;Flatscher-Bader and Wilce, 2006;Tew et al, 2007;Collins-Racie et al, 2009;Schäfer et al, 2009;Sun et al, 2013;Aregger et al, 2014;Hueging et al, 2015;Kliuchnikova et al, 2016;…”

Section: Apod Systematic Review Reference Collectionsunclassified

The Lipocalin Apolipoprotein D Functional Portrait: A Systematic Review

Sánchez

Ganfornina

2021

Front. Physiol.

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Apolipoprotein D is a chordate gene early originated in the Lipocalin protein family. Among other features, regulation of its expression in a wide variety of disease conditions in humans, as apparently unrelated as neurodegeneration or breast cancer, have called for attention on this gene. Also, its presence in different tissues, from blood to brain, and different subcellular locations, from HDL lipoparticles to the interior of lysosomes or the surface of extracellular vesicles, poses an interesting challenge in deciphering its physiological function: Is ApoD a moonlighting protein, serving different roles in different cellular compartments, tissues, or organisms? Or does it have a unique biochemical mechanism of action that accounts for such apparently diverse roles in different physiological situations? To answer these questions, we have performed a systematic review of all primary publications where ApoD properties have been investigated in chordates. We conclude that ApoD ligand binding in the Lipocalin pocket, combined with an antioxidant activity performed at the rim of the pocket are properties sufficient to explain ApoD association with different lipid-based structures, where its physiological function is better described as lipid-management than by long-range lipid-transport. Controlling the redox state of these lipid structures in particular subcellular locations or extracellular structures, ApoD is able to modulate an enormous array of apparently diverse processes in the organism, both in health and disease. The new picture emerging from these data should help to put the physiological role of ApoD in new contexts and to inspire well-focused future research.

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Endometrial expression of selected genes in patients achieving pregnancy spontaneously or after ICSI and patients failing at least two ICSI cycles

Cited by 10 publications

References 70 publications

Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers

Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers

DNA methylation changes in endometrium and correlation with gene expression during the transition from pre-receptive to receptive phase

The Lipocalin Apolipoprotein D Functional Portrait: A Systematic Review

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