Endometrial gene expression profiling of recurrent implantation failure after in vitro fertilization

Albayrak, İrem Gülfem; Azhari, Fatemeh; Çolak, Ezgi Nur; Balcı, Burçin Karamustafaoğlu; Ülgen, Ege; Sezerman, Uğur; Baştu, Ercan; Günel, Tuba

doi:10.1007/s11033-021-06502-x

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…As reported in previous studies, RIF is closely related to endometrial receptivity and related proteins in the endometrium expressed abnormally in patients with RIF [ 5 , 15 ]. miRNA is a small endogenous RNA and a key regulator of posttranscriptional gene expression and candidate genes acting as biomarkers in diseases [ 16 ].…”

Section: Discussionmentioning

confidence: 84%

Comprehensive Analysis of circRNA-Mediated ceRNA Regulatory Networks in relation to Recurrent Implantation Failure

Wang

Zhu

2022

Evidence-Based Complementary and Alternative Medicine

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Recurrent implantation failure (RIF) is attributed to endometrial receptivity dysfunction with many unanswered questions. Up to now, there is no explanation for RIF, and therapeutic strategies are usually limited to supportive care. In this study, we differentially analyzed the raw data deposited in three eligible microarray datasets, GSE111974, GSE121219, and GSE147442 to screen DE-mRNAs, DE-miRNAs, and DE-circRNAs, respectively. The value of log2-fold change |log2FC| ≥ 1 and the adjusted p value < 0.05 were considered differentially expressed between RIF and fertile control. We found 350 DE-mRNAs, 43 DE-miRNAs, and 1968 DE-circRNAs between RIF and fertile control. The PPI network identified 6 hub genes with degree ≥10, KDR, AGT, POSTN, TOP2A, RRM2, and PTGS2, in RIF. KDR, AGT, POSTN, TOP2A, and RRM2 were downregulated in endometrial tissue samples of RIF compared with those of fertile control, while PTGS2 was upregulated in endometrial tissue samples of RIF compared with those of fertile control. According to the ceRNA hypothesis, 15 groups of ceRNA network based on 10 circRNAs, hsa_circ_001572, hsa_circ_001884, hsa_circ_001375, hsa_circ_001449, hsa_circ_000029, hsa_circ_001168, hsa_circ_000210, hsa_circ_001484, hsa_circ_001698, and hsa_circ_000089 were constructed in RIF. In conclusion, the present study examined the possible role of circRNAs and their related ceRNA network involved in the pathogenesis of RIF.

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Section: Discussionmentioning

confidence: 84%

Comprehensive Analysis of circRNA-Mediated ceRNA Regulatory Networks in relation to Recurrent Implantation Failure

Wang

Zhu

2022

Evidence-Based Complementary and Alternative Medicine

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“…37,38 To understand endometrial defects in women RIF, transcriptome profiles of the endometrium from women with and without RIF have been compared. The results reveal altered expression of several coding genes, [39][40][41] long noncoding RNA, 42 and miRNAs 43 in the endometrium of women with RIF. Altered levels of several mRNA and miRNAs are also observed in extracellular vesicles isolated from women with RIF.…”

Section: Rif Is An Endometrial Defectmentioning

confidence: 94%

“…30,41,[44][45][46] Network analysis of the transcriptome data has identified disruptions in circadian clock pathways, Hippo signaling pathway, renin-angiotensin system pathway, cell adhesion, regulation of cell motility, and cellular response to vascular endothelial growth factor stimulus, and several others. 39,40 Functionally these alterations can affect endometrial receptivity, lead to defective decidualization, and alterations in endometrial immune profiles in the endometrium of women with RIF. 7,[47][48][49] Thus, it is becoming increasingly clear that RIF is an endometrial defect.…”

Section: Rif Is An Endometrial Defectmentioning

confidence: 99%

Immune alterations in recurrent implantation failure

Mukherjee

Sharma

Modi

2022

American J Rep Immunol

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A failure to achieve pregnancy after three or more embryo transfer cycles with high‐quality blastocysts is referred to as recurrent implantation failure (RIF). RIF can be due to altered uterine factors or male factors or embryo factors. Disrupted endometrial receptivity, altered expression of genes in several pathways, immunologic disturbances in the peripheral blood and/or the endometrium, and epigenetic alterations are associated with RIF. Amongst the immunologic disturbances, altered Th1/Th2 ratio, altered NK cell and macrophage numbers are observed in women with RIF. However, not all women with RIF have the same kind of immune dysfunction suggesting that RIF is a heterogeneous condition associated with varied immune responses and one size may not fit all. Thus, personalized therapies based on the immune status of the patient are being tested in women with RIF. In general, women with a high Th1/Th2 ratio are offered Tacrolimus, while intravenous IgG is recommended in women with high NK cell numbers/HLA mismatch. Women with hyperactivated immune status in the uterus are offered progesterone support, prednisolone, vitamin E, and intralipid treatment to suppress inflammation and oxidative stress, while endometrial scratching and intrauterine hCG administration are offered to women with hypo‐active immune status. There is a need for standardized tests for evaluation of immune status in patients and sufficiently powered randomized controlled trials for personalized therapies to determine which of these will be beneficial in women with RIF. Till then, the ART community should limit the use of such add‐on interventions in women with RIF.

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“…In addition, the ICAM-1 gene and MUC1 gene play crucial roles in cellular adhesion in the endometrium, however, it has been proven that genetic polymorphism in the ICAM-1 and MUC1 domain may be proven that genetic polymorphisms in the ICAM-1 and MUC1 domains may be associated with susceptibility to endometriosis, leading to implantation failure or endometriosis, recurrence (4). In addition, the expression of other altered genes, including those encoding MME and WWP1, were greatly enhanced, whereas the expression of the gene encoding TNC, LIF and HOXA-10 were decreased in recurrent implantation failure samples during the implantation window (5,6). Collectively, these altered genes may serve as targets for improving endometrial receptivity.…”

Section: Femalementioning

confidence: 99%

Drug substitution and adjuvant therapy in patients with genetics related infertility: A review

Jin

Zhou

et al. 2023

DD&T

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With the in-depth study of the human genome and the increasing popularity of gene sequencing, it has been gradually confirmed that genetics can play a crucial role in infertility. To provide references for clinical treatment, we have focused on genes and drug therapy for genetic infertility. This review recommends adjuvant therapy and drug substitution. Examples of these therapies include antioxidants (such as folic acid, vitamin D, vitamin E, inositol, coenzyme Q10 etc.), metformin, anticoagulants, levothyroxine, dehydroepiandrosterone, glucocorticoids, and gonadotropins etc. Based on the pathogenesis, we provide an overview of the current knowledge, including randomized controlled trials and systematic reviews, and predict potential target genes and signaling pathways, proposing possible future strategies for the use of targeted drugs to treat infertility. Non-coding RNAs are anticipated to become a novel target for the treatment of reproductive illnesses since they have a significant role in controlling the occurrence and development of reproductive diseases.

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Endometrial gene expression profiling of recurrent implantation failure after in vitro fertilization

Cited by 11 publications

References 34 publications

Comprehensive Analysis of circRNA-Mediated ceRNA Regulatory Networks in relation to Recurrent Implantation Failure

Comprehensive Analysis of circRNA-Mediated ceRNA Regulatory Networks in relation to Recurrent Implantation Failure

Immune alterations in recurrent implantation failure

Drug substitution and adjuvant therapy in patients with genetics related infertility: A review

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