Endometrial lymphoid tissue: an immunohistological study.

Morris, Huw; Edwards, Joshua; Tiltman, A. J.; Emms, M.

doi:10.1136/jcp.38.6.644

Cited by 119 publications

(62 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies are under way to determine whether differences seen with whole bacteria and PAMPs are due to bacterial exclusion. Previous studies in our laboratory and by others have shown that the epithelium of the uterus, similar to epithelia at other mucosal sites, is supported by an underlying population of immune cells that are dispersed as well as present as lymphoid aggregates made up of a B-cell core surrounded by T cells with an outer halo of macrophages (27,38,60). The hypothesis that CCL20 production by epithelial cells plays a role in the recruitment of immune cells to the female reproductive tract was suggested by knockout studies of CCR6, the unique receptor for CCL20 (7,55).…”

Section: Vol 73 2005mentioning

confidence: 97%

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys

Crane-Godreau¹,

Wira²

2005

Infect Immun

View full text Add to dashboard Cite

Having previously shown that CCL20/macrophage inflammatory protein 3␣ and tumor necrosis factor alpha (TNF-␣) are released by polarized primary rat uterine epithelial cells (UEC) in response to Escherichia coli but not to Lactobacillus rhamnosus, we sought to determine if epithelial cells are responsive to pathogen-associated molecular patterns (PAMP), including lipopolysaccharide (LPS), lipoteichoic acid (LTA), and Pam 3 Cys, a bacterial lipoprotein analog. Epithelial cells were grown to confluence on Nunc cell culture inserts prior to apical treatment with PAMPs. In response to LPS, LTA, and Pam 3 Cys (EMC Microcollection GmbH, Tü-bingen, Germany), CCL20 levels increased (4-to 10-fold) while PAMPs caused increased TNF-␣ (1-to 4-fold) in the medium collected after 24 h of incubation. Both apical and basolateral secretion of CCL20 and TNF-␣ increased in response to PAMPs, but treatments had no effect on cell viability and integrity, as measured by transepithelial resistance. Time course studies of CCL20 and TNF-␣ release in response to Pam 3 Cys and LPS indicated that CCL20 release peaked between 2 and 4 h after treatment, whereas TNF-␣ release was gradual over the length of the incubation. Freeze-thaw and cell lysis experiments, along with actinomycin D studies, suggested that CCL20 and TNF-␣ are synthesized in response to PAMP stimulation. Taken together, these studies demonstrate that E. coli and selected PAMPs have direct effects on the production of CCL20 and TNF-␣ without affecting cell integrity. Since CCL20 is known to be both chemotactic and antimicrobial, the increase in apical and basolateral release by UEC in response to PAMPs suggests a new mechanism of innate immune protection in the female reproductive tract.

show abstract

Section: Vol 73 2005mentioning

confidence: 97%

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys

Crane-Godreau¹,

Wira²

2005

Infect Immun

View full text Add to dashboard Cite

show abstract

“…17,18 Because APC are MHC-II+, detecting MHC-II expression in the reproductive tract could provide useful information about the presence and localization of APC. In studies of rats 19 and humans, 4,7,20 MHC-II+ cells were found in the female reproductive tract. In rabbits, although macrophages have been found in the endometrium, 15 their distribution in the lower reproductive tract and the distribution of other MHC-II+ cells in the whole reproductive tract remains unknown.…”

Section: Introductionmentioning

confidence: 99%

“…T cells are the dominant lymphocytes in the female reproductive tract of humans, [4][5][6][7] mice, 8,9 and cows, 10 and only a few B cells are present in the reproductive tracts of these species. 11 However, IgG and IgA plasma cells have been reported in the reproductive tract of female mice 12,13 and IgA plasma cells together with a few IgG plasma cells have been described in the human fallopian tube.…”

Section: Introductionmentioning

confidence: 99%

Lymphocytes and MHC class II positive cells in the female rabbit reproductive tract before and after ovulation

Janssens

Holland

et al. 2005

Immunol Cell Biol

View full text Add to dashboard Cite

SummaryIn this study, we identified lymphocytes and MHC class II positive (MHC-II+) cells in the reproductive tract of female rabbits both before and after ovulation. CD43+ T cells were frequently present in the mucosa of the oviduct, cervix, and vagina, but far fewer positive cells were seen in the endometrium. The induction of ovulation did not change the cell density in these regions. KEN-5+ T cells and MHC-II+ cells were also frequently seen in the mucosa of the oviduct, cervix, and vagina both before and after ovulation. However, in the uterus, there were very few positive cells before ovulation, but the number increased dramatically after ovulation. Associated with the increase of KEN-5+ T cells, IL-2 mRNA expression in the uterus also increased after ovulation, suggesting that the uterus experienced an increase of T-cell activation. IgM-and IgA-positive B cells were not commonly seen in the reproductive tract and the induction of ovulation did not alter this. Our results suggest that the reproductive tract of female rabbits has the capacity to mount an immune response and that the immune cell distribution of the rabbit reproductive tract has some distinctive features compared with that found in other species.

show abstract

“…According to our findings, short-term unidirectional signals for the antigenic dissimilarity of foetuses seem to be sufficient for induction of the immuno-endocrine mechanism responsible for the additional increases in plasma progesterone during implantation. A key role in this mechanism might be the production of c h o r i o n i c g o n a d o t r o p h i n b y p e r i p h e r a l mononuclear cells that form mucosa associated lymphocyte tissues (MALT) in the fallopian tubes and endometrium [41,42]. Local or general allogenic stimulation induces sec retion of gonadotrophin by mononuclear cells [43] that can enhance the increase in plasma progesterone.…”

Section: Discussionmentioning

confidence: 99%

Allogenic Stimulation in Early Pregnancy Improves Pre- and Postnatal Ontogenesis in BALB/cLac Mice.

Gerlinskaya

Мошкин

Evsikov

2000

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. The influence of allogenic stimulation on pregnancy and the development of progeny was studied in BALB/cLac female mice that were injected with C57BL/6J male (allogenic) red blood cells (AB) on the second day after intrastrain mating. On the 4 th day of pregnancy AB-treated females had higher levels of plasma progesterone than syngenic blood (SB) treated females and controls (salinetreated). Administration of allogenic erythrocytes also stimulated embryo development and improved maternal behaviour. On the day of birth the incidence of new-born rejection was much lower in AB-treated mice than in SB-treated mice A battery of physiological and behavioural tests were used to assess the adaptive features of BALB/cLac males born to females injected with AB, SB or saline. These offspring groups did not differ in parameters of maximum aerobic performance, cold resistance or humoral immune response. At the same time, offspring of antigenically challenged mothers had less adrenocortical and behavioural responses to emotional stressors than offspring of SB and saline treated females. AB males also had higher mating success than other male groups.

show abstract

Endometrial lymphoid tissue: an immunohistological study.

Cited by 119 publications

References 9 publications

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys

Lymphocytes and MHC class II positive cells in the female rabbit reproductive tract before and after ovulation

Allogenic Stimulation in Early Pregnancy Improves Pre- and Postnatal Ontogenesis in BALB/cLac Mice.

Contact Info

Product

Resources

About

Endometrial lymphoid tissue: an immunohistological study.

Cited by 119 publications

References 9 publications

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam3Cys

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam3Cys

Lymphocytes and MHC class II positive cells in the female rabbit reproductive tract before and after ovulation

Allogenic Stimulation in Early Pregnancy Improves Pre- and Postnatal Ontogenesis in BALB/cLac Mice.

Contact Info

Product

Resources

About

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys

CCL20/Macrophage Inflammatory Protein 3α and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment with Lipopolysaccharide or Pam₃Cys