Endophytes: exploitation as a tool in plant protection

Dutta, Devanushi; Puzari, K. C.; Gogoi, Robin; Dutta, Pranab

doi:10.1590/s1516-8913201402043

Cited by 140 publications

(67 citation statements)

References 48 publications

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“…Endophytes are microorganisms that symbiotically colonized within plant tissues for all or a part of their life cycle without causing any harmful effect to their host (Hirsch and Braun, 1992; Wilson, 1995). Fungal endophytes benefit and offer several advantages to their host by preventing them from desiccation, promoting plant growth hormone, increasing nutrients acquisition, protecting from insect, pathogenic fungi and environmental harsh conditions (Dutta et al, 2014;Nair and Padmavathy, 2014). Meanwhile, the host plant would provide the endophytes with shelters, space for their colonization and also nutrients acquired by them (Saikkonen et al, 2004;Schardl et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Taufiq¹,

Darah²

2019

J Pure Appl Microbiol

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Citation: M.M.J. Taufiq and I. Darah, Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System, J Pure Appl Microbiol., 2019; 13(4):Abstract The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0±0.1 U/mL and 1.46±0.1 g/L of fungal growth were obtained after incorporating all the improved cultural conditions which consisted of yeast extract sucrose broth supplemented with host plant extract, with initial medium pH of 6, 2 mycelial plugs of 4 days old seed culture, cultivation temperature of 30°C and cultivated for 16 days under static conditions without the presence of light. After improvement of cultural conditions, a significant increment of anti-MRSA activity of about 57.57% (2.4 folds) and a slight increment of fungal growth of about 8. 15% (1.1 folds) were obtained compared to the cultural condition before improvement. Indeed the improvement of cultural conditions greatly affected the anti-MRSA activity and growth production by L. pseudotheobromae IBRL OS-64 isolate.

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Section: Discussionmentioning

confidence: 99%

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Taufiq¹,

Darah²

2019

J Pure Appl Microbiol

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“…When tested against different pathogenic bacteria, such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli (Ratnaweera et al, 2015) and different fungi and yeasts, including Candida albicans (Premjanu et al, 2016), it demonstrated a strong antimicrobial action. Thus, compounds produced by endophytic fungi of plants with recognized biological activity for biotechnological purposes can be applied in the alternative control of urban and agricultural pests (Dutta et al, 2014), in place of the toxic chemicals sold on the commercial market.…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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“…Endophytes are the group of microorganisms that live inside host microenvironment, receive protection from environmental stresses, face lesser competition from other microbes and have greater access to nutrients [1]. Since endophytes interact closer to plant than rhizosphere and phyllosphere bacteria, their effects to the plants may be direct and intense.…”

Section: Introductionmentioning

confidence: 99%