Endophytic Bacteria Potentially Promote Plant Growth by Synthesizing Different Metabolites and their Phenotypic/Physiological Profiles in the Biolog GEN III MicroPlateTM Test

Abstract

Endophytic bacteria, as the most promising components of effective, biofertilizers biostimulating and biocontrol preparations, should be very intensively obtained from various plants and studied in terms of the conditions determining the potential ability to promote plant growth. For this reason, endophytic bacteria have been isolated from both stems and roots of up to six systematically distant species of vascular plants: one species belonging to the seedless vascular plants (Monilophyta), and five seed plants (Spermatophyta). The 23 isolated strains represented nine genera: Delftia, Stenotrophomonas, Rhizobium, Brevundimonas, Variovorax, Achromobacter, Novosphingobium, Comamonas and Collimonas, notably which were closely related—belonging to the phylum Proteobacteria. Stenotrophomonas sp. strains showed the greatest ability to synthesize indole-3-acetic acid (IAA)-like compounds, while Achromobacter sp. strains produced the highest levels of siderophores. The presence of the nifH gene and nitrogen binding activity was demonstrated for 95% of the strains tested. Stenotrophomonas maltophila (ES2 strain) showed the highest metabolic activity based on Biolog GEN III test. The ability to solubilize phosphate was determined only for three tested strains from genus: Delftia, Rhizobium and Novosphingobium. The presented work demonstrated that the metabolic and phenotypic properties of plant growth-promoting endophytes are correlated with the genus of bacteria and are not correlated with the host plant species or part of plant (stem, root).

Bacterial etiology of necrotic spots on leaves and shoots of grapevine (Vitis vinifera L.) in Poland

“…viticola was identified as Xanthomonas perforans. This supports the method limitations in bacteria identification (Adriko et al 2016, Prieto-Calvo et al 2016 as also emerged from the recent work of Woźniak et al (2019) that, comparing identification of endophytic bacteria isolates using Biolog GEN III plates and 16S rRNA gene sequences, pointed out that a degree of uncertainty especially in case of the metabolically lessactive isolates.…”

Novel Effects of Leonardite-Based Applications on Sugar Beet

“…These are Duganella , Stenotrophomonas , Ralstonia , Delftia , Microbacterium , Acidovorax , Aurantimonas , Spirosoma , and Rhizobium. They can be mostly divided into disease suppressive, such as Duganella , Microbacterium , Rhizobium , Delftia , and Stenotrophomonas that also have beneficial activity on plant growth and, on the other hand, Acidovorax and Ralstonia are recognized to be plant pathogens ( Bergna et al, 2018 ; Woźniak et al, 2019 ).…”

Investigation of ACC-Deaminase and Indole Acetic Acid Producing Bacteria from Rhizospheric Soils in Ağrı Province

“…IAA production is one of the most effective PGP mechanism (Pawlik et al, 2017 (Phetcharat and Duangpaeng 2012;Vinayarani and Prakash 2018). There are many reports have been shown IAA production by rhizospheric and endophytic bacteria (Ray et al, 2016;Chowdhury et al, 2017;Vinayarani and Prakash 2018;Wozniak et al, 2019). Various rhizospheric bacteria have potential to produce the auxin phytohormone IAA, these kind of rhizospheric microorganisms produce auxins in the presence of a favourable precursor like L-tryptophane (Phetcharat and Duangpaeng 2012).…”