Endophytic Bacterium Serratia plymuthica From Chinese Leek Suppressed Apple Ring Rot on Postharvest Apple Fruit

Sun, Meng; Liu, Junping; Li, Jinghui; Huang, Yonghong

doi:10.3389/fmicb.2021.802887

Cited by 19 publications

(9 citation statements)

References 58 publications

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“…gave the best results for the inhibition test by production of volatile substances. Similar results were obtained by [57,58,59,60]. According to several studies, one of the mechanisms of action of soil bacteria against phytopathogens is linked to the production of biomolecules (HCN, NH3) and lytic enzymes such as cellulase, proteases, chitinases.…”

Section: Discussionsupporting

confidence: 81%

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Ould Ouali,

Houali,

Cruz

et al. 2024

Preprint

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Salinity is one of the major factors responsible for the deterioration of soils, making them unsuitable for agriculture. The application of halotolerant and halophilic plant growth promoting bacteria (PGPR: Plant Growth Promoting Rhizobacteria) with biological control activities as an inoculant of cultivated plants offers a biological alternative to the use of agrochemicals, particularly when subjected to salt stress. In this perspective, 70 bacterial strains were isolated from saline soils (sebkha) in arid and semi-arid areas of eastern Algeria. Three isolates were selected based on their ability to produce bioactive molecules allowing them to promote plant growth, such as hydrolytic enzymes, indole acetic acid, HCN, NH3, etc. Two of these isolates belong to the genus Serratia and the third is a halophilic Halomonas bacteria. These bacteria were identified based on the 16S rDNA sequence. Antagonism tests against phytopathogenic fungi were carried out. The identification of the antifungal molecules produced by these bacteria was done by high performance liquid chromatography. These bacteria are capable of inhibiting mycelial development against phytopathogenic fungi with rates reaching 80.67% against Botrytis cinerea, 76.22% against Aspergillus niger and 66.67% against Fusarium culmorum for Serratia sp. The strain Halomonas sp. inhibited mycelial growth by production of volatile substances of Aspergillus niger at 71.29%, Aspergillus flavus at 75.49% and Penicillium glabrum at a rate of 72.22%. The identification by HPLC of the antifungal molecules produced by these three bacteria reveals that they are polyphenols, which makes these strains the first rhizobacteria capable of producing phenolic compounds. Finally, pot tests to determine the effectiveness of these strains in promoting wheat growth under salinity stress (125 mM, 150 mM and 200 mM) were carried out. The results revealed that a consortium of two isolates (Serratia sp. and Halomonas sp.) performed best at 125 mM. But, at higher concentrations it was the halophilic bacteria Halomonas sp which gave the best result. In all cases, there was a significant improvement in the growth of wheat seedlings inoculated with the bacteria, compared to non-inoculated controls.

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Section: Discussionsupporting

confidence: 81%

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Ould Ouali,

Houali,

Cruz

et al. 2024

Preprint

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“…Given that fact, we inferred that there were components or endophytic bacterium with antifungal activity in Chinese leek plants. Fortunately, we corroborated that the dimethyl trisulfide, one of the main components of Chinese leek volatiles (Sun et al, 2022a), and the endophytic bacterium Serratia plymuthica isolated from Chinese leek (Sun et al, 2022b), showed strong inhibitory effect against B. dothidea. In the present study, we validated that another endophytic bacterium P. protegens, isolated from Chinese leek, had a significant inhibitory effect on B. dothidea and strikingly reduced the apple ring rot on postharvest fruits.…”

Section: Discussionsupporting

confidence: 81%

Endophytic bacterium Pseudomonas protegens suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest fruits

Huang

Liu

et al. 2022

Front. Microbiol.

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Apple (Malus domestica Borkh.), one of the most economically important fruits widely consumed worldwide, has been suffering from apple ring rot caused by Botryosphaeria dothidea, which dramatically affects its quality and yield. In the present study, we demonstrated that Pseudomonas protegens, isolated from Chinese leek (Allium tuberosum), significantly suppressed the mycelial growth and propagation of B. dothidea, respectively, further displayed a considerably inhibitory effect on the apple ring rot of postharvest fruits. In addition, P. protegens significantly improved the total soluble solid/titrable acidity (TSS/TA) ratio and soluble sugar/titrable acidity (SS/TA) ratio and drastically maintained the fruit firmness. Further analysis manifested that P. protegens substantially induced the defense-related genes such as MdGLU, MdPAL, MdPOD, MdCAL, and transcription factors related to the resistance to B. dothidea, including MdWRKY15, MdPUB29, MdMyb73, and MdERF11 in apple fruits. Meanwhile, P. protegens considerably restrained the expressions of the pathogenicity-related genes in B. dothidea, including the BdCYP450, BdADH, BdGHY, BdATS, Bdα/β-HY, and BdSTR. By inference, P. protegens inhibited the apple ring rot on postharvest fruits by activating the defense system of apple fruit and repressing the pathogenic factor of B. dothidea. The study provided a theoretical basis and a potential alternative to manage the apple ring rot on postharvest fruits.

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“…In horticultural plants, the research on fruit diseases mainly focuses on postharvest fungal diseases, such as blue and green molds of citrus [ 27 ], ring rot disease of apple [ 28 ], and grey mold of tomato [ 29 ] etc. These pathogenic fungi are usually inoculated by injecting suspended spore solution or inserting agar disk containing mycelium.…”

Section: Discussionmentioning

confidence: 99%

Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation

Liu,

Liu

et al. 2024

Plant Methods

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Background Citrus canker is a significant bacterial disease caused by Xanthomonas citri subsp. citri (Xcc) that severely impedes the healthy development of the citrus industry. Especially when citrus fruit is infected by Xcc, it will reduce or even lost its commercial value. However, due to the prolonged fruiting cycle and intricate structure, much less research progress had been made in canker disease on fruit than on leaf. In fact, limited understanding has been achieved on canker development and the response to Xcc infection in fruit. Results Herein, the progression of canker disease on sweet orange fruit was tracked in the field. Results indicated that typical lesions initially appear on the sepal, style residue, nectary disk, epicarp, and peduncle of young fruits after petal fall. The susceptibility of fruits to Xcc infection diminished as the fruit developed, with no new lesions forming at the ripening stage. The establishment of an efficient method for inoculating Xcc on fruit as well as the artificial inoculation throughout the fruit's developmental cycle clarified this infection pattern. Additionally, microscopic observations during the infection process revealed that Xcc invasion caused structural changes on the surface and cross-section of the fruit. Conclusions An efficient system for inoculation on citrus fruit with Xcc was established, by which it can serve for the evaluation of citrus germplasm for canker disease resistance and systematic research on the interactions between Xcc and citrus fruits.

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Endophytic Bacterium Serratia plymuthica From Chinese Leek Suppressed Apple Ring Rot on Postharvest Apple Fruit

Cited by 19 publications

References 58 publications

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Endophytic bacterium Pseudomonas protegens suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest fruits

Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation

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