Endophytic fungal production rates of volatile organic compounds are highest under microaerophilic conditions

Schoen, Heidi R.; Knighton, W. B.; Peyton, Brent M.

doi:10.1099/mic.0.000555

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…Our proposed method yielded diverse VOC profiles for most tested filamentous fungi. The closed headspace vial in the method likely results in microaerophilic conditions, which facilitates VOC production of endophytes [37].…”

Section: Performance Of Proposed Cultivation Techniquementioning

confidence: 99%

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

et al. 2020

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The interaction between plant defensive metabolites and different plant-associated fungal species is of high interest to many disciplines. Volatile organic compounds (VOCs) are natural products that are easily evaporated under ambient conditions. They play a very important role in inter-species communication of microbes and their hosts. In this study, the VOCs produced by 43 different fungal isolates of endophytic and soil fungi during growth on horseradish root (Armoracia rusticana) extract or malt extract agar were examined, by using headspace-gas chromatography-mass spectrometry (headspace-GC-MS) and a high relative surface agar film as a medium. The proposed technique enabled sensitive detection of several typical VOCs (acetone, methyl acetate, methyl formate, ethyl acetate, methyl butanol isomers, styrene, beta-phellandrene), along with glucosinolate decomposition products, including allyl cyanide and allyl isothiocyanate and other sulfur-containing compounds—carbon disulfide, dimethyl sulfide. The VOC patterns of fungi belonging to Setophoma, Paraphoma, Plectosphaerella, Pyrenochaeta, Volutella, Cadophora, Notophoma, and Curvularia genera were described for the first time. The VOC pattern was significantly different among the isolates. The pattern was indicative of putative myrosinase activity for many tested isolates. On the other hand, endophytes and soil fungi as groups could not be separated by VOC pattern or intensity.

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Section: Performance Of Proposed Cultivation Techniquementioning

confidence: 99%

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

et al. 2020

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“…All in vivo FH yields were at least 10 4 -fold lower than theoretical limits, which indicated production was not for catabolic purposes but rather served other biological functions, like perhaps, communication molecules. Feedback mechanisms in metabolism and physiology, such O 2 concentration and carbon source, may control the production of specific FHs in vivo [47,48]. Previous reports indicate that some plant tissues also have metabolic shifts at~10% O 2 partial pressure [49].…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of Functionalized Hydrocarbon Production Using Ehrlich Pathway and Fatty Acid Derivatives in an Endophytic Fungus

Hunt

Mallette

Peyton

et al. 2021

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Functionalized hydrocarbons have various ecological and industrial uses, from signaling molecules and antifungal/antibacterial agents to fuels and specialty chemicals. The potential to produce functionalized hydrocarbons using the cellulolytic, endophytic fungus, Ascocoryne sarcoides, was quantified using genome-enabled, stoichiometric modeling. In silico analysis identified available routes to produce these hydrocarbons, including both anabolic- and catabolic-associated strategies, and determined correlations between the type and size of the hydrocarbons and culturing conditions. The analysis quantified the limits of the wild-type metabolic network to produce functionalized hydrocarbons from cellulose-based substrates and identified metabolic engineering targets, including cellobiose phosphorylase (CP) and cytosolic pyruvate dehydrogenase complex (PDHcyt). CP and PDHcyt activity increased the theoretical production limits under anoxic conditions where less energy was extracted from the substrate. The incorporation of both engineering targets resulted in near-complete conservation of substrate electrons in functionalized hydrocarbons. The in silico framework was integrated with in vitro fungal batch growth experiments to support O2 limitation and functionalized hydrocarbon production predictions. The metabolic reconstruction of this endophytic filamentous fungus describes pathways for both specific and general production strategies of 161 functionalized hydrocarbons applicable to many eukaryotic hosts.

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“…[99], Muscodor albus [101] and Nodulisporium sp. [102]. Another direct MS technique is selected ion flow tube-mass spectrometry (SIFT-MS) that has been adapted for the detection of volatile traces from medically important fungi such as species of Aspergillus, Candida, Cryptococcus, Fusarium and Mucor [103][104][105].…”

Section: Identification Of Vocs Of Fungal Originmentioning

confidence: 99%

Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk

Calla-Quispe

Fuentes-Rivera

Ramírez

et al. 2020

Life

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Fungi are a highly diverse group of heterotrophic organisms that play an important role in diverse ecological interactions, many of which are chemically mediated. Fungi have a very versatile metabolism, which allows them to synthesize a large number of still little-known chemical compounds, such as soluble compounds that are secreted into the medium and volatile compounds that are chemical mediators over short and long distances. Mass spectrometry (MS) is currently playing a dominant role in mycological studies, mainly due to its inherent sensitivity and rapid identification capabilities of different metabolites. Furthermore, MS has also been used as a reliable and accurate tool for fungi identification (i.e., biotyping). Here, we introduce the readers about fungal specialized metabolites, their role in ecological interactions and provide an overview on the MS-based techniques used in fungal studies. We particularly present the importance of sampling techniques, strategies to reduce false-positive identification and new MS-based analytical strategies that can be used in mycological studies, further expanding the use of MS in broader applications. Therefore, we foresee a bright future for mass spectrometry-based research in the field of mycology.

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Endophytic fungal production rates of volatile organic compounds are highest under microaerophilic conditions

Cited by 7 publications

References 44 publications

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

In Silico Analysis of Functionalized Hydrocarbon Production Using Ehrlich Pathway and Fatty Acid Derivatives in an Endophytic Fungus

Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk

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