Endophytic fungi: a potential source for drugs against central nervous system disorders

Pant, Anushree; Vasundhara, M.

doi:10.1007/s42770-023-00997-1

Cited by 3 publications

(2 citation statements)

References 138 publications

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“…After quality filtering, a total of 1,114,159 high-quality sequences with an average sequence length of 240 bp were extracted from the samples. The OTU abundance was normalized using the sample containing the least sequences (31,120). At a 97% similarity level, 1190 OTUs were obtained, removing the ones matching to the sequence of chloroplasts and mitochondria (Table S1).…”

Section: Analysis Of Operational Taxonomic Units (Otus)mentioning

confidence: 99%

“…Pharmaceuticals such as huperzine A and galantamine, currently on the market, are identified as superior sources of acetylcholinesterase inhibitors [ 29 , 30 ]. More and more research has found that endophytic fungi are becoming a significant source of AChE inhibitors [ 31 , 32 , 33 ], and it is essential to pay more attention to the diversity and AChE-inhibitory activity of endophytic fungi in P. crotalarioides . Normally, P. crotalarioides must develop for at least two years before it may be utilized medically, because locals in Yunnan believe that two-year-old plants can accumulate enough medicinal ingredients to fully exert the therapeutic effect.…”

Section: Introductionmentioning

confidence: 99%

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Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods

Shen,

Xiong,

Liu

et al. 2024

JoF

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Polygala crotalarioides, a perennial herbaceous plant found in southwest China, has the potential to be used in the treatment of Alzheimer’s disease. Endophytic fungi that reside within medicinal herbs play an important ecological role in their host plants and can serve as a valuable source for identifying active components. However, little is known about the diversity, and structure of endophytic fungi in P. crotalarioides. In this study, we investigated the community structure and diversity of endophytic fungi in the leaves, stems, and roots of P. crotalarioides at both 1- and 2-year-growth stages using a modern culture-independent method using both culture-independent (high-throughput sequencing, HTS) and culture-based methods. Using HTS, our results revealed that the richness and diversity of endophytic fungi in P. crotalarioides varied depending on the organs and growth stages. Specifically, stems and leaves exhibited significantly higher diversity compared to roots. Additionally, the highest diversity of endophytic fungi was observed in the stems of the 2-year-old plants. At the genus level, Fusarium, Colletotrichum, and Phoma were the most abundant endophytic fungi in 1-year-old samples, while Cercospora, Apiotrichum, and Fusarium were prevalent in 2-year-old samples. A total of 55 endophytic fungal strains belonging to two phyla and 24 genera were isolated from 150 plant tissue segments using culture-based methods. The anti-acetylcholinesterase activity of these isolates was evaluated in vitro and five of them, Phialophora mustea PCAM010, Diaporthe nobilis PCBM027, Fusarium oxysporum LP41, F. oxysporum SR60, and Phoma herbarum SM81, showed strong activity (>50% inhibition rate). These findings will serve as a theoretical basis and practical guide for comprehending the structural composition, biological diversity and bioactivity of endophytic fungi in P. crotalarioides.

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Section: Analysis Of Operational Taxonomic Units (Otus)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods

Shen,

Xiong,

Liu

et al. 2024

JoF

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Current status of research on endophytes of traditional Tibetan medicinal plant and their metabolites

Hu,

Long,

Luobu

et al. 2023

3 Biotech

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Altenuene derivatives produced by an endophyte Alternaria alternata

Takahashi,

Isshiki,

Hakozaki

et al. 2024

Natural Product Research

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Three novel aromatic polyketides 1 and 3 and five known compounds, (4S,10S)-talaroflavone (2), altenuene (4), isoaltenuene (5), alternariol (6), and altenusin (7), were isolated from an endophytic strain of Alternaria alternata SI-694. The structures of the new compounds, including their absolute configurations, were elucidated by NMR, IR, UV, and ECD spectroscopies, and phytotoxicities of the isolated compounds were also evaluated. Altenusin (7) showed moderate cytotoxicity against HL-60 cells, with an IC50 of 6.65 M, whereas 5, 6, and 7 were phytotoxic against Lactuca sativa, Brassica campestris L, Stellaria aquatica (L.) Scop. and Digitaria ciliaris.

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Endophytic fungi: a potential source for drugs against central nervous system disorders

Cited by 3 publications

References 138 publications

Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods

Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods

Current status of research on endophytes of traditional Tibetan medicinal plant and their metabolites

Altenuene derivatives produced by an endophyte Alternaria alternata

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