Endophytic Fungi and Their Enzymatic Potential

Mishra, Rashmi; Kushveer, J. S.; Revanthbabu, P.; Sarma, V. Venkateswara

doi:10.1007/978-3-030-03589-1_14

Cited by 19 publications

(8 citation statements)

References 111 publications

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“…Endophytic fungi produce lytic enzymes such as cellulases, pectinases, amylases, phosphatases, lipases and proteases (Mishra et al, 2019), which help endophytes establish symbiotic associations with host plants (Hallmann et al, 1997) and suppress plant pathogen activities (Gao et al, 2010). These associations have (e.g., auxin, gibberellins), and hydrolytic enzymes (e.g., cellulases, proteases, chitinases) important for the survival and maintenance of endophytes in plants and for host plant health and tolerance to stressful environments (Eid et al, 2019).…”

Section: Endophytic Fungi As Sources Of Hydrolytic Enzymesmentioning

confidence: 99%

Endophytic fungi: an overview on biotechnological and agronomic potential

et al. 2024

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Endophytic fungi colonize the inter- and/or intracellular regions of healthy plant tissues and have a close symbiotic relationship with their hosts. These microorganisms produce antibiotics, enzymes, and other bioactive compounds that enable them to survive in competitive habitats with other microorganisms. In addition, secondary metabolites confer protection to their host plant against other bacterial and fungal pathogens and/or can promote plant growth. Endophytic fungi are viewed as a promising source of bioactive natural products, which can be optimized through changes in growing conditions. The exploration of novel bioactive molecules produced by these microorganisms has been attracting attention from researchers. The chemical and functional diversity of natural products from endophytic fungi exhibits a broad spectrum of applications in medicine, agriculture, industry and the environment. Fungal endophytes can also enhance the photoprotective effects and photochemical efficiency in the host plants. Modern omic approaches have facilitated research investigating symbiotic plant-endophytic fungi interactions. Therefore, research on endophytic fungi can help discovery novel biomolecules for various biotechnological applications and develop a sustainable agriculture.

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Section: Endophytic Fungi As Sources Of Hydrolytic Enzymesmentioning

confidence: 99%

Endophytic fungi: an overview on biotechnological and agronomic potential

et al. 2024

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“…Therefore, fungal endophytes find immense application as bioremediating agents. Mishra et al (2019) have systematically reviewed the hydrolytic and oxidative enzymatic potential of endophytic fungal candidates in metabolizing scalable transformation of non-selective and diverse chemical substrates. Bioprospecting endophytic fungal enzymes (manganese peroxidase, lignin peroxidase, cellulase, chitinase, laccase, lipoidase, etc.)…”

Section: Applications Of Endophytic Fungi As Biocatalystmentioning

confidence: 99%

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Choudhary

Gupta

Dhar

et al. 2021

Front. Bioeng. Biotechnol.

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Catalysis is a process carried out in the presence of a heterogenous catalyst for accelerating the rate of a chemical reaction. It plays a pivotal role in transition from take, make, and dispose technology to sustainable technology via chemo- and biocatalytic processes. However, chemocatalyzed reactions are usually associated with copious amounts of perilous/hazardous environmental footprints. Therefore, whole-cell biotransformations or enzyme cocktails serve as cleaner biocatalytic alternatives in replacing the classical chemical procedures. These benchmark bioconversion reactions serve as important key technology in achieving the goals of green chemistry by eliminating waste generation at source. For this, nature has always been a driving force in fuelling natural product discovery and related applications. The fungal endophytic community, in particular, has undergone co-evolution with their host plant and has emerged as a powerful tool of genetic diversity. They can serve as a treasure trove of biocatalysts, catalyzing organic transformations of a wide range of substances into enantiopure compounds with biotechnological relevance. Additionally, the biocatalytic potential of endophytic fungi as whole-intact organisms/isolated enzyme systems has been greatly expanded beyond the existing boundaries with the advancement in high-throughput screening, molecular biology techniques, metabolic engineering, and protein engineering. Therefore, the present review illustrates the promising applications of endophytic fungi as biocatalysts for the synthesis of new structural analogs and pharmaceutical intermediates and refinement of existing proteins for novel chemistries.

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“…The definition of endophytes reveals that they reside within the host plant so there is a huge chance that endophytes could be potent tannase producers and also they promote plants' adaptation in new environments. 5 In the present investigation fungal endophytes of a common ethnomedicinal plant Andrographis paniculata have been screened for the production of industrially valuable extracellular hydrolytic enzymes, i.e. protease, lipase, amylase, laccase and tannase.…”

Section: Introductionmentioning

confidence: 99%

Exploitation of Selected Fungal Endophytes of Andrographis paniculata (Burm. f.) Wall. ex Nees for the Production and Optimisation of Tannase and Screening for their Associated Hydrolysing Enzymes; Amylase, Protease, Lipase, and Laccase

Mishra,

Santra,

Banerjee

2024

J. Pure Appl. Microbiol.

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Endophytes represent a diverse domain of microorganisms with immense biotechnological potentialities. Metabolites from endophytes, especially fungi, are useful in industrial as well as pharmaceutical aspects. Here, endophytic fungal isolates of Andrographis paniculata collected from the Tapobon, Junglemahal region of West Bengal, India, have been studied for their hydrolyzing enzyme production abilities. Out of eighty-one isolates, twenty-one were positive for amylase, protease, lipase, laccase, and tannase action. Microscopic features of positive isolates revealed that the enzyme producers were Aspergillus sp., Fusarium sp., Alternaria sp., Trichoderma sp., Exerohilum sp., Nigrospora sp., Curvularia sp., Cladosporium sp., Cochliobolus sp., Tricothecium sp., Penicillium sp., Verticillium sp., and Cephalosporium sp. The amylolytic activity was remarkable in the case of endophytic Aspergillus sp. and Fusarium sp. Also, Aspergillus sp. and Alternaria sp. had proteolytic activity. Aspergillus sp., Mycelia sterilia-2, and Trichoderma sp. were potent lipase producers. Along with Exerohilum sp. endophytic Aspergillus sp. had positive laccase activity. The tannic acid degrading activity was highest for Aspergillus sp. APL11 followed by Helicosporium sp. and Fusarium sp. Tannase producing ability of Aspergillus sp. was optimized and an incubation time- 96h, incubation temperature- 29°C, initial medium pH- 6.5, carbon source- glucose, and nitrogen source- NaNO3 exhibited a two-fold scale-up of (2.83±0.05 u mL-1) in tannase action. These enzymes offer eco-friendly and efficient solutions across a wide range of industrial processes including food production, textile processing, and pharmaceutical synthesis. Fungal endophytes of medicinal plants act as an alternative source for hydrolysing enzymes.

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Endophytic Fungi and Their Enzymatic Potential

Cited by 19 publications

References 111 publications

Endophytic fungi: an overview on biotechnological and agronomic potential

Endophytic fungi: an overview on biotechnological and agronomic potential

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Exploitation of Selected Fungal Endophytes of Andrographis paniculata (Burm. f.) Wall. ex Nees for the Production and Optimisation of Tannase and Screening for their Associated Hydrolysing Enzymes; Amylase, Protease, Lipase, and Laccase

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