Endophytic fungi associated with soybean plants and their antagonistic activity against Rhizoctonia solani

Abstract

Background Fungal endophytes produce many secondary metabolites that can reduce root rot diseases. Soybean is a particularly important crop worldwide. Endophytic fungi can be isolated, identified, and incorporated into sustainable agriculture for the biological control of many diseases. Results The aim of this study was to isolate some endophytic fungi for controlling the most important diseases of soybean plants and to study the mechanisms underlying this biocontrol regarding the suppression of pathogens. Ten endophytic fungi were isolated from soybean plants. Among them, the 3 fungi isolates that exhibited a high percentage of antagonistic activity against Rhizoctonia solani, the causal pathogen of root rot disease of soybean plants, were identified as Trichoderma longibrachiatum S12, T. asperellum S11, and T. atroviride PHYTAT7. The 3 fungi isolates had the ability to produce pectinase and chitinase and to solubilize phosphors. Moreover, they produced siderophores and indole acetic acid (IAA), which have a strong effect on the growth of the plants. The 3 isolates reduced disease severity by 64, 60, and 55%, respectively than the infected control. Conclusion The results suggest that certain endophytic fungi associated with soybean plants have potential for the management of root rot diseases in soybean. Moreover, these isolates can be considered as having a growth-promoting effect in soybean plants.

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

“…The antifungal role of the lytic activity of Trichoderma species was reported against the growth of Rhizoctonia solani pathogen. The isolate can produce pectinase and chitinase and solubilize phosphorus (Sallam et al, 2021).…”

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

“…Trichoderma asperellum , T . atroviride and T. longibrachiatum isolated from soybean ( Glycine max ) were shown to reduce infection of seeds by the pathogen Rhizoctonia solani by 64, 60 and 55%, respectively, when applied in solution to infected soils [ 85 ]. The Trichoderma species produced the hydrolytic enzymes pectinase and chitinase, all capable of degrading cell wall components [ 85 ].…”

“…atroviride and T. longibrachiatum isolated from soybean ( Glycine max ) were shown to reduce infection of seeds by the pathogen Rhizoctonia solani by 64, 60 and 55%, respectively, when applied in solution to infected soils [ 85 ]. The Trichoderma species produced the hydrolytic enzymes pectinase and chitinase, all capable of degrading cell wall components [ 85 ]. Additionally, the Trichoderma species produced siderophores, which reduce the availability of iron to pathogenic fungi, and IAA, which has a strong effect on plant growth [ 85 ].…”

“…The Trichoderma species produced the hydrolytic enzymes pectinase and chitinase, all capable of degrading cell wall components [ 85 ]. Additionally, the Trichoderma species produced siderophores, which reduce the availability of iron to pathogenic fungi, and IAA, which has a strong effect on plant growth [ 85 ]. Trichoderma erinaceum isolated from ginger and Stemona root was shown to inhibit the growth of the southern stem rot disease agent Sclerotium rolfsii by 64% in dual culture assays and reduce infection by 58% in pot experiments [ 78 ].…”

Scaling-up production of cost-effective and eco-friendly bio-fertilizer and its application on Barley green fodder via IoT hydroponic system

“…These microorganisms play a key role in enhancing plant growth and crop productivity by generating high-quality bioactive metabolites [1][2][3]. Therefore, these endophytes were applied as bio-fertilizers, bio-pesticides, or bio-remediators [3][4][5][6][7]. To increase crop production by using cheaper alternative solutions, efficient endophytes have prospected recently [8].…”