Endophytic fungi: expanding the arsenal of industrial enzyme producers

Corrêa, Rúbia Carvalho Gomes; Rhoden, Sandro Augusto; Mota, Thatiane Rodrigues; Azevedo, João Lúcio; Pamphile, João Alencar; Souza, Cristina Giatti Marques de; Polizeli, Maria de Lourdes Teixeira de Moraes; Bracht, Adelar; Peralta, Rosane Marina

doi:10.1007/s10295-014-1496-2

Cited by 107 publications

(60 citation statements)

References 107 publications

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“…To look for the presence of more complex or higher order enzyme substrate processes within the on-QD format, assays were also performed in the presence of increasing concentrations of the competitive inhibitor triethyl phosphate (TPE). 47 Figure 5 presents representative 3-D kinetic plots of initial rates as the amount of TPE increases for free enzyme, 525 QD-(PTE) 3 …”

Section: Articlementioning

confidence: 99%

Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency

Breger

Ancona²,

Walper³

et al. 2015

ACS Nano

173

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As a specific example of the enhancement of enzymatic activity that can be induced by nanoparticles, we investigate the hydrolysis of the organophosphate paraoxon by phosphotriesterase (PTE) when the latter is displayed on semiconductor quantum dots (QDs). PTE conjugation to QDs underwent extensive characterization including structural simulations, electrophoretic mobility shift assays, and dynamic light scattering to confirm orientational and ratiometric control over enzyme display which appears to be necessary for enhancement. PTE hydrolytic activity was then examined when attached to ca. 4 and 9 nm diameter QDs in comparison to controls of freely diffusing enzyme alone. The results confirm that the activity of the QD conjugates significantly exceeded that of freely diffusing PTE in both initial rate (∼4-fold) and enzymatic efficiency (∼2-fold). To probe kinetic acceleration, various modified assays including those with increased temperature, presence of a competitive inhibitor, and increased viscosity were undertaken to measure the activation energy and dissociation rates. Cumulatively, the data indicate that the higher activity is due to an acceleration in enzyme-product dissociation that is presumably driven by the markedly different microenvironment of the PTE-QD bioconjugate's hydration layer. This report highlights how a specific change in an enzymatic mechanism can be both identified and directly linked to its enhanced activity when displayed on a nanoparticle. Moreover, the generality of the mechanism suggests that it could well be responsible for other examples of nanoparticle-enhanced catalysis.

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Section: Articlementioning

confidence: 99%

Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency

Breger

Ancona²,

Walper³

et al. 2015

ACS Nano

173

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“…The experimental response of these process variables was the production of glucose oxidase (U/ml). Prevalent in other environments such as water (Sessegolo et al, 2011;Oliveira et al, 2016), plants (Pinheiro et al, 2013;Zhou et al, 2016;Corrêa et al, 2014;Russell et al, 2011;Orlandelli et al, 2012), marine organisms (Debbab et al, 2012;Subramani et al, 2013;Hayashi et al, 2016;Cicatiello et al, 2016) and even the glacial arctic environment (Sonjak et al, 2006;Sonjak et al, 2007;Zhang et al, 2016). A fraction of the fungal isolates (20%) were able to produce GOx.…”

Section: Discussionmentioning

confidence: 99%

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Sousa¹,

Santos²,

Cortez³

et al. 2017

Afr. J. Microbiol. Res.

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Glucose oxidase (GOx) has several industrial applications. It is believed that there are several species of fungi that have the ability to produce this enzyme, most of which are unexplored. This work aimed to investigate the production of glucose oxidase (EC 1.1.3.4) by fungi isolated from soil samples of the Amazonian forest. Filamentous fungi were isolated from soil samples from the Adolpho Ducke Forest Reserve, located in Manaus, Amazonas. Strains were subjected to submerged bioprocessing to select for the best GOx producers. Those selected for the production of isolated enzymes were subjected to kinetic tests that evaluated production of the enzyme and consumption of the biomass substrate by the isolates. In addition, experiments to evaluate the optimal carbon, nitrogen and phosphorus sources as well as the influence of the bioprocess factors were carried out. Finally, GOx production was investigated in a semi-continuous system for 7 days. The most frequent isolates isolated from soil samples belonged to the genera Aspergillus, Penicillium and Trichoderma. Aspergillus niger LMM01 was the best GOx producer. Glucose, peptone and KH 2 PO 4 were demonstrated to be the optimal carbon, nitrogen and phosphorus sources, respectively. Multivariate experiments demonstrated that the parameters with the greatest effect on GOx production were pH and agitation. Stable expression results for GOx (7.74 U/ml) were obtained over 7 days in a semi-continuous process. In this context, the new Amazonian source of this enzyme (A. niger LMM01), and enzyme production in a semi-continuous process, demonstrates the importance of the present work.

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“…Although the filamentous fungal strains have a remarkable capacity of secreting extracellular proteins, filamentous endophytes remain under-exploited as enzymatic sources (Corrêa et al, 2014). These fungi colonize intra-and inter-cellular plant tissues, establishing a harmonious relationship without causing any apparent damage to the host plant (Fouda, Hassan, Eid, & Ewais, 2015).…”

Section: Introductionmentioning

confidence: 99%

<b>Use of agro-industrial wastes as substrates for α-amylase production by endophytic fungi isolated from <i>Piper hispidum</i> Sw

et al. 2017

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ABSTRACT. Millions of tons of corn, pineapple, sugarcane and wheat are annually produced and their industrial processing generates large amounts of wastes. Current study evaluated the use of corncob (CC), pineapple peel (PP), sugarcane bagasse (SB) and wheat bran (WB) as substrates for α-amylase production by submerged cultures of nine endophytes from Piper hispidum Sw. Initially, fungi were grown on a starch medium and the cup plate assay showed that five strains were amylase-positive: Bipolaris sp. JF767001, Marasmius cladophyllus JF767003, Phlebia sp. JF766997, Phyllosticta capitalensis JF766988 and Schizophyllum commune JF766994, with enzymatic halos ranging between 6.30 ± 0.08 and 8.87 ± 0.68 mm in diameter. Further, the use of agroindustrial wastes was evaluated by the cup plate assay, where the highest halo diameters were obtained from fungi grown on PP or SB: up to 15.00 ± 0.16 mm (Phlebia sp.) and 14.80 ± 0.18 mm (S. commune), respectively. Submerged cultures with PP or SB showed the highest levels of α-amylase activity according to the starch-iodine assay, or rather, up to 4.14 ± 0.02 U mL -1 (Bipolaris sp.) and 4.09 ± 0.02 U mL -1 (Phlebia sp.), respectively. Results give an in-depth knowledge on tropical endophytes that might be α-amylase sources, and indicate the suitability of these agro-industrial wastes as substrates for fungal enzymatic production.Keywords: amylolytic activity, endophytes, microbial enzymes, submerged fermentation.Uso de resíduos agroindustriais para a produção de α-amilase por fungos endofíticos isolados de Piper hispidum Sw RESUMO. Milhões de toneladas de milho, abacaxi, cana-de-açúcar e trigo são anualmente produzidas e seu processamento industrial gera grandes quantidades de resíduos. Este estudo avaliou o uso de sabugo de milho (SM), casca de abacaxi (CA), bagaço de cana-de-açúcar (BC) e farelo de trigo (FT) como substratos para a produção de amilase em culturas submersas de nove endófitos de Piper hispidum Sw. Inicialmente, os fungos foram crescidos em amido e o ensaio cup plate mostrou resultados positivos para cinco fungos: Bipolaris sp. JF767001, Marasmius cladophyllus JF767003, Phlebia sp. JF766997, Phyllosticta capitalensis JF766988 e Schizophyllum commune JF766994, com halos enzimáticos de 6,30 ± 0,08 a 8,87 ± 0,68 mm. Posteriormente, o uso dos resíduos foi avaliado pelo ensaio cup plate, em que os maiores halos foram obtidos para CA ou BC: até 15,00 ± 0,16 mm (Phlebia sp.) e 14,80 ± 0,18 mm (S. commune), respectivamente. As culturas submersas com CA ou BC também apresentaram os maiores níveis de α-amilase de acordo com a reação dextrinizante: até 4,14 ± 0,02 U mL -1 (Bipolaris sp.) e 4,09 ± 0,02 U mL -1 (Phlebia sp.), respectivamente. Os resultados expandem o conhecimento sobre endófitos de regiões tropicais que são fontes de α-amilase, indicando que os referidos resíduos são adequados para a produção enzimática de tais fungos.Palavras-chave: atividade amilolítica, endófitos, enzimas microbianas, fermentação submersa.

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Endophytic fungi: expanding the arsenal of industrial enzyme producers

Cited by 107 publications

References 107 publications

Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency

Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

<b>Use of agro-industrial wastes as substrates for α-amylase production by endophytic fungi isolated from <i>Piper hispidum</i> Sw

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