Endophytic fungus Diaporthe caatingaensis MT192326 from Buchanania axillaris: An indicator to produce biocontrol agents in plant protection

Dhakshinamoorthy, M.; Kannan, Karthik; Kumar, P. Senthil; Saravanakumar, Tamilselvi

doi:10.1016/j.envres.2021.111147

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…The yield was lower than that previously reported for Fusarium solani strain ATLOY-8 12 and Diaporthe caatingaensis MT192326. 47 However, its anticancer activity was more potent than others, such as the F. solani MTCC9667 strain from A. dimidiate 31 and P. chrysogenum . 15 The difference might depend on the enzyme system involved in the CPT biosynthetic pathway in screening CPT-producing endophytic fungi.…”

Section: Discussionmentioning

confidence: 94%

Isolation, anticancer potency, and camptothecin—producing ability of endophytic fungi isolated from Ixora chinensis

Doan,

Le,

et al. 2024

Science Progress

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Camptothecin (CPT) is an important alkaloid used for anticancer treatment. It is mainly produced by two endangered and overharvested Camptotheca acuminata and Nothapodytes nimmoniana plants. Endophytic fungi are promising alternative sources for CPT production. In the present study, fungi residing within explants of Ixora chinensis were isolated and their CPT-producing capability of their endophytes was verified via thin-layer chromatography, high-performance liquid chromatography, liquid chromatography/high resolution mass spectrometry, and nuclear magnetic resonance analyses and compared with standards. In addition, MTT and sulforhodamine B assays were selected to test the anticancer effect. The endophytic fungi collection of 62 isolates were assigned to 11 genera, with four common genera ( Diaporthe, Phyllosticta, Colletotrichum, and Phomopsis) and seven less common genera ( Penicillium, Botryosphaeria, Fusarium, Pestalotiopsis, Aspergillus, and Didymella). Moreover, the anticancer activity of extracts was assessed against human lung carcinoma (A549). Among eight potential extracts, only Penicillium sp. I3R2 was found to be a source of CPT, while the remaining seven extracts have not been discovered potential secondary compounds. Thus, other prominent endophytic fungi might be potential candidates of phytochemicals with anticancer properties.

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Section: Discussionmentioning

confidence: 94%

Isolation, anticancer potency, and camptothecin—producing ability of endophytic fungi isolated from Ixora chinensis

Doan,

Le,

et al. 2024

Science Progress

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“…For example, the endophytic fungus Diaporthe phaseolorum isolated from tomatoes can produce 16 different compounds, and it has an inhibitory effect on the growth of Xanthomonas vesicatoria, which causes bacterial spot disease in tomatoes [ 115 ]. Dhakshinamoorthy et al [ 116 ] showed that the endophytic fungus Diaporthe caatingaensis from Buchanania axillaris leaves can produce the bioactive metabolite Camptothecin (CPT). While our endophyte Diaporthe sp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Karunarathna

Hyde

et al. 2022

JoF

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Coffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tissues collected in four coffee plantations in Pu’er city, Yunnan province, China. Molecular identification was carried out using maximum likelihood phylogenetic analysis of nuclear ribosomal internal transcribed spacer (ITS1-5.8S rDNA-ITS2) sequences, while the colonization rate and the isolation frequency were also calculated. Two pathogenic fungi (Alternaria alternata and Penicillium digitatum) and two pathogenic bacteria (Pseudomonas syringae and Salmonella enterica subsp. enterica) were used for screening the antagonistic activities of 61 strains of coffee-endophytic fungi by a dual-culture test assay while maximum likelihood phylogenetic analysis confirmed their natural classification. This is the first study of coffee-leaf-endophytic fungal diversity in China, and the results revealed that coffee-endophytic fungi from this study belong to the Ascomycota, distributed among two classes, 10 orders, and 17 families. Concurrently, endophytic fungi isolates distributed in Arthrinium, Biscogniauxia, Daldinia, Diaporthe, and Nigrospora showed strong antagonistic activities against the pathogens. For the pathogens Alternaria alternata and Pseudomonas syringae, Nigrospora XCE-7 showed the best inhibitory effects with inhibition rates of 71.76% and 61.11%, respectively. For the pathogen Penicillium digitatum, Daldinia ME-9 showed the best inhibitory effect with a 74.67% inhibition rate, while Biscogniauxia PTE-7 and Daldinia T5E-1-3 showed the best inhibitory effect with a rate of 60.42% against the pathogen Salmonella enterica subsp. enterica. Overall, our study shows the diversity of coffee endophytes in four coffee-growing areas in Pu’er city, Yunnan province, China, and their potential use as biological control agents against two fungal and two bacterial pathogens.

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“…Phyllosticta capitalensis was found to produce 1-octacosanol, an antioxidant and antibacterial compound [ 68 ]. Diaporthe caatingaensis isolated from the medicinal plant Buchanania axillaris was found to produce camptothecin, a molecule more commonly derived from plants, that has anticancer, antibacterial and antifungal properties [ 89 , 90 ]. Further study is required to determine if the fungal-derived camptothecin shares the same antifungal properties as the plant-derived compound.…”

Section: Fungal Endophytes and Their Effects On Fungal Pathogensmentioning

confidence: 99%

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

Grabka

d’Entremont

Adams

et al. 2022

Plants

107

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Virtually all examined plant species harbour fungal endophytes which asymptomatically infect or colonize living plant tissues, including leaves, branches, stems and roots. Endophyte-host interactions are complex and span the mutualist–pathogen continuum. Notably, mutualist endophytes can confer increased fitness to their host plants compared with uncolonized plants, which has attracted interest in their potential application in integrated plant health management strategies. In this review, we report on the many benefits that fungal endophytes provide to agricultural plants against common non-insect pests such as fungi, bacteria, nematodes, viruses, and mites. We report endophytic modes of action against the aforementioned pests and describe why this broad group of fungi is vitally important to current and future agricultural practices. We also list an extensive number of plant-friendly endophytes and detail where they are most commonly found or applied in different studies. This review acts as a general resource for understanding endophytes as they relate to potential large-scale agricultural applications.

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Endophytic fungus Diaporthe caatingaensis MT192326 from Buchanania axillaris: An indicator to produce biocontrol agents in plant protection

Cited by 12 publications

References 36 publications

Isolation, anticancer potency, and camptothecin—producing ability of endophytic fungi isolated from Ixora chinensis

Isolation, anticancer potency, and camptothecin—producing ability of endophytic fungi isolated from Ixora chinensis

Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Fungal Endophytes and Their Role in Agricultural Plant Protection against Pests and Pathogens

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