Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study

Corsetti, Giovanni; Romano, Claudia; Stacchiotti, Alessandra; Pasini, Evasio; Dioguardi, Francesco Saverio

doi:10.1007/s12011-016-0912-z

Cited by 44 publications

(22 citation statements)

References 65 publications

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“…This distribution of reactive microglia is analogous with previously published data on their band-like accumulation in regions surrounding the ischemic core during the first week of ischemic stroke ( Li et al, 2013 , 2014 ), indicating that instead of containing pre-existing microglia, the lesion core was progressively infiltrated by microglia migrating from the peri-lesional area. These activated microglia/macrophages showed intense immunoreactivity for GRP78, as previously reported ( Fan et al, 2015 ; Yang et al, 2015 ; Corsetti et al, 2017 ; Jin et al, 2018a ). Thus, the temporal patterns of vascular GRP78 appeared to overlap with the period during which GRP78 was expressed by microglia/macrophages recruited into the lesion core.…”

Section: Discussionsupporting

confidence: 87%

Spatiotemporal Expression of GRP78 in the Blood Vessels of Rats Treated With 3-Nitropropionic Acid Correlates With Blood–Brain Barrier Disruption

Jin

Riew

Kim

et al. 2018

Front. Cell. Neurosci.

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Glucose-regulated protein (GRP78) or BiP, a 78-kDa chaperone protein located in the endoplasmic reticulum (ER), has recently been reported to be involved in the neuroglial response to ischemia-induced ER stress. The present study was designed to study the expression patterns of this protein and the cell types involved in the induction of GRP78 expression in rats treated with the mitochondrial toxin 3-nitropropionic acid (3-NP). GRP78 immunoreactivity was almost exclusively localized to striatal neurons in saline-treated controls, but GRP78 expression was induced in activated glial cells, including reactive astrocytes and activated microglia/macrophages, in the striata of rats treated with 3-NP. In the lesion core, increased GRP78 immunoreactivity was observed in the vasculature; this was evident in the lesion periphery of the core at 3 days after lesion induction, and was evenly distributed throughout the lesion core by 7 days after lesion induction. Vascular GRP78 expression was correlated, both temporally and spatially, with infiltration of activated microglia into the lesion core. In addition, this was coincident with the time and pattern of blood–brain barrier (BBB) leakage, detected by the extravasation of fluorescein isothiocyanate-albumin, an established BBB permeability marker. Vascular GRP78-positive cells in the lesion core were identified as endothelial cells, smooth muscle cells, and adventitial fibroblast-like cells, in which GRP78 protein was specifically localized to the cisternae of the rough ER and perinuclear cisternae, but not to other organelles such as mitochondria or nuclei. Thus, our data provide novel insights into the phenotypic and functional heterogeneity of GRP78-positive cells within the lesion core, suggesting the involvement of GRP78 in the activation/recruitment of activated microglia/macrophages and its potential role in BBB impairment in response to a 3-NP-mediated neurotoxic insult.

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Section: Discussionsupporting

confidence: 87%

Spatiotemporal Expression of GRP78 in the Blood Vessels of Rats Treated With 3-Nitropropionic Acid Correlates With Blood–Brain Barrier Disruption

Jin

Riew

Kim

et al. 2018

Front. Cell. Neurosci.

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“…In contrast, molecular analysis, although much more sensitive in highlighting proteins and very often used exclusively, does require immediate freezing and homogenization of the sample. As a consequence, it does not take into account the specificities of protein location, such as tissue morphology and organization [94]. This is a major limitation in the exclusive use of molecular analysis, which we believe is comparable if not superior to IHC.…”

Section: Study Limitationsmentioning

confidence: 97%

Dietary Modifications of Nitrogen Intake Decreases Inflammation and Promotes Rejuvenation of Spleen in Aged Mice

Romano¹,

Corsetti²,

Pasini³

et al. 2018

JFNR

Self Cite

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The spleen is a lymphoid organ with multiple functions including blood filtration and immune activity.Aging changes the spleen's anatomy for both immune and stromal cells and can lead to immunesenescence, contributing to the increased rates of mortality and morbidity commonly observed in the elderly. Much evidence indicates that the combination of food quantity and quality can influence chronic inflammatory states in the spleen. Quantitative amino-acids (AA) adequacy is pivotal to maintain cell integrity in mammals. Aged mice feed with balanced essential-AA (EAA) formulation improved mitochondrial biogenesis and morphological and molecular changes in many organs, as well as increased lifespan. Here, we evaluated the inflammatory state of the spleen in aged male mice (fifteen months old) chronically fed for twelve months with a particular EAA-rich diet compared to a standard laboratory diet. This study found that chronic consumption of an EAA-rich diet decreased inflammation and modulated reticular and mitochondrial chaperones, mitochondrial function and cells survival, maintaining the correct architecture of the spleen. These changes could also be beneficial for immune system integrity, providing a possible theoretical-speculative basis for the role of EAA improving the quality of life of the elderly by probably slowing immune-senescence.

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“…In a similar study, Aldahmash et al [32] reported lead intake to cause severe alterations in the kidney and spleen, which was manifested by hepatocytes degeneration, leukocytic infiltration, ill-defined architecture of the spleen, presence of large macrophages and lymphoid necrosis. Hypocellular white pulp, enlargement of venous sinusoids, clustering of heterochromatin in the nucleus, vacuolation in the cytoplasm, swelling of mitochondria and complete distortion of rough endoplasmic reticulum cisterns are also some of the effects of lead [33,34].…”

Section: Resultsmentioning

confidence: 99%

Effects of Shiga-Toxin Producing Strains of Escherichia coli (STEC), Lead and Bisphenol A Mixture on Kidney and Spleen of Whistler Mice

Omoruyi

Enabulele

Omogbare

2018

JST

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Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study

Cited by 44 publications

References 65 publications

Spatiotemporal Expression of GRP78 in the Blood Vessels of Rats Treated With 3-Nitropropionic Acid Correlates With Blood–Brain Barrier Disruption

Spatiotemporal Expression of GRP78 in the Blood Vessels of Rats Treated With 3-Nitropropionic Acid Correlates With Blood–Brain Barrier Disruption

Dietary Modifications of Nitrogen Intake Decreases Inflammation and Promotes Rejuvenation of Spleen in Aged Mice

Effects of Shiga-Toxin Producing Strains of Escherichia coli (STEC), Lead and Bisphenol A Mixture on Kidney and Spleen of Whistler Mice

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