Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

Skorobagatko, Daria A.; Mazilov, A. A.; Strashnyuk, V. Yu.

doi:10.1007/s00411-019-00828-8

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Дослідження впливу іонізуючого випромінювання на різні аспекти життєдiяльності організмів має тривалу історію. Багато уваги приділяється вивченню фізіологічних (Einor et al, 2016), генетичних (Alexander, Bergendahl, 1964;Golub, Chernyk, 2008;Skorobagatko et al, 2020), у тому числі епігенетичних (Vaiserman, 2011;Guerrero-Bosagna et al, 2014) наслідків опромінення. Більшість досліджень було проведено в лабораторних умовах, але, як відзначають різні автори, їх результати не можна перенести на популяційні процеси (Козерецкая и др., 2008;Kivisaari et al, 2020).…”

Section: вступunclassified

Selection parameters in lines of Drosophila melanogaster Meig., obtained from populations living in territories with different levels of radiation pollution: approbation of the Crow’s method

Lukianov,

Zlatiev,

Vakulenko

et al. 2023

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

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Man-made disasters, such as the accidents at the Chornobyl NPP and the Fukushima NPP-1, have raised questions about the radiation risks associated with the use of atomic energy in a new and acute way. The purpose of the study was to investigate the features of the action of natural selection in the lines of Drosophila melanogaster Meig., obtained from natural populations living in territories with different levels of radiation pollution. One of the aims was to test Crow's method on a model object, such as Drosophila. This method makes it possible to estimate the total intensity of selection, as well as to determine the contribution of its individual components, such as differential fecundity and differential mortality. The study was carried out on three lines of Drosophila melanogaster Meig.: the Haidary line (radiation background in the territory from which the line originates: 0.12 μSv/h, β-radiation flux: 0 particles/cm2/min), the KhPTI line (radiation background: 0.12–0.20 μSv/h, β-radiation flux: 0 particles/cm2/min), the Chornobyl line (radiation background: 0.20–0.22 μSv/h, β-radiation flux: 7–8 particles/cm2/min). According to the obtained results, the lines did not differ among themselves in egg production of females. In terms of the number of adult offspring, the Chornobyl line was inferior to the Haidary and KhPTI lines by 48.9% and 57.8%, respectively. Mortality in the pre-reproductive period of development (indicator pd), which includes embryonic and pupal mortality, was the highest in the Chornobyl line and exceeded the pd value in the Haidary and KhPTI lines by 1.4 times. As a result, the Chornobyl line, obtained from the territory contaminated with radionuclides, significantly exceeded the Haidary and KhPTI lines, obtained from the territories where the radiation situation does not go beyond the norm, by both components of selection – both by the component of differential fecundity (If), and by differential mortality (lm). The total selection indices (Itot) were quite close in the Haidary and KhPTI lines, and in the Chornobyl line this index was 2,1–2,6 times higher than in the other two lines. The results of the study support the view that ionizing radiation can promote evolution by accelerating evolutionary change. They indicate an increased mortality rate, a reduced level of fitness and an increased selection pressure in the line of fruit flies, which originates from the population living in the radiation-contaminated territory in the Chornobyl exclusion zone.

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Section: вступunclassified

Selection parameters in lines of Drosophila melanogaster Meig., obtained from populations living in territories with different levels of radiation pollution: approbation of the Crow’s method

Lukianov,

Zlatiev,

Vakulenko

et al. 2023

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

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“…Відомо, що ендоцикли сприяють прискореному росту [10], реагуванню на фізіологічний стрес [11][12][13] та адаптації до умов навколишнього середовища [13,14], регуляції гомеостазу та регенерації тканин [15]. Ендоредуплікація лежить в основі альтернативного проліферації так званого ауксетичного способу росту.…”

Section: результати та обговоренняunclassified

Reproduction and endoreduplication in Drosophila melanogaster Meig. when influenced by lead nitrate

Strashnyuk¹,

Taglina²

2020

Fakt. eksp. evol. org.

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Aim. The purpose of investigation was to study the reproductive ability and polyteny degree of chromosomes in Drosophila melanogaster Meig. under the influence of various concentrations of lead nitrate. Methods. Canton-S wild-type strain was used as the material. Flies developed on standard sugar-yeast medium, to which in the experiment lead nitrate was added in concentrations of 0.1, 1, and 10 mg/ml. The reproductive ability of the strain was evaluated by the number of adult offspring. The polyteny degree of chromosomes was studied on squashed preparations of larva salivary glands stained with acetoorsein by cytomorphometry. The preparations were obtained at late 3rd instar. Results. The number of adult offsprings decreased when lead nitrate was added to the nutrient medium: at a concentration of 0.1 mg/ml – by 22.8 %, at 1 mg/ml – by 38.9 %. A concentration of 10 mg/ml was lethal. Males showed greater sensitivity to the drug compared to females. The degree of polyteny of chromosomes in the salivary glands of larvae decreased on average by 5.0–6.5 %. Conclusions. Lead nitrate causes a significant, dose-dependent decrease in the reproductive ability of fruit flies and has a toxic effect on Drosophila cells, inhibiting the process of endoreduplication. Keywords: Drosophila melanogaster Meig., heavy metals, fecundity, giant chromosomes, polyteny.

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Genotoxic and mutational potential of monocyclic terpenoids (carvacrol, carvone and thymol) in Drosophila melanogaster

et al. 2023

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Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

Cited by 3 publications

References 47 publications

Selection parameters in lines of Drosophila melanogaster Meig., obtained from populations living in territories with different levels of radiation pollution: approbation of the Crow’s method

Selection parameters in lines of Drosophila melanogaster Meig., obtained from populations living in territories with different levels of radiation pollution: approbation of the Crow’s method

Reproduction and endoreduplication in Drosophila melanogaster Meig. when influenced by lead nitrate

Genotoxic and mutational potential of monocyclic terpenoids (carvacrol, carvone and thymol) in Drosophila melanogaster

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