Endoreduplication Occurs during Pod Wall Development in Temperate Grain Legumes

Lagunes-Espinoza, Luz del Carmen; Huyghe, C.; Bousseau, David; Barre, Phillippe; Papineau, J.

doi:10.1006/anbo.2000.1174

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Furthermore, a correlation between cell differentiation and endoreduplication has been observed during pod wall development in Lupinus (Lagunes-Espinoza et al, 2000) and nodule (Ashton et al, 1979): no accumulation of cells in G1 in the mutant. Fig.…”

Section: Endopolyploidisationmentioning

confidence: 84%

Unique Tissue‐Specific Cell Cycle in Physcomitrella

Schween

Gorr

Hohe³

et al. 2003

Plant Biology

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The moss Physcomitrella patens (Hedw.) B.S.G. is a novel tool in plant functional genomics as it has an inimitable high gene targeting efficiency facilitating the establishment of gene/function relationships. Here we report, based on flow cytrometric (FCM) data, that the basic nuclear DNA content per cell of Physcomitrella is 0.53 pg, equating to a genome size of 1 C = 511 Mbp. Furthermore, we describe a unique tissue‐specific cell cycle change in this plant. Young plants consisting of only one cell type (chloronema) displayed one single peak of fluorescence in FCM analyses. As soon as the second cell type (caulonema) developed from chloronema, a second peak of fluorescence at half the intensity of the previous one became detectable, indicating that caulonema cells were predominantly at the G1/S transition, whereas chloronema cells were mainly accumulating at the G2/M transition. This conclusion was validated by further evidence: i) The addition of ammonium tartrate arrested Physcomitrella in the chloronema state and in G2/M. ii) Two different developmental mutants, known to be arrested in the chloronema/caulonema transition, remained in G2/M, regardless of age and treatment. iii) The addition of auxin or cytokinin induced the formation of caulonema, as well as decreasing the amount of cells in G2/M phase. Additionally, plant growth regulators promoted endopolyploidisation. Thus, cell cycle and cell differentiation are closely linked in Physcomitrella and effects of plant hormones and environmental factors on both processes can be analysed in a straight forward way. We speculate that this unique tissue‐specific cell cycle arrest may be the reason for the uniquely high rate of homologous recombination found in the Physcomitrella nuclear DNA.

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Section: Endopolyploidisationmentioning

confidence: 84%

Unique Tissue‐Specific Cell Cycle in Physcomitrella

Schween

Gorr

Hohe³

et al. 2003

Plant Biology

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“…Further evidence was found in legumes where cell differentiation to a specialized function as pod wall tissues was accompanied by endoreduplication, and higher ploidy levels coincided with maximum pod growth [29]. During tomato fruit development, the pericarp tissue of young green fruit did not have higher ploidy (usually within 2C and 4C), but most of the cells in pericarp became endopolyploid (up to 256C) as the fruit developed further [6].…”

Section: Discussionmentioning

confidence: 99%

Systemic endopolyploidy in Spathoglottis plicata (Orchidaceae) development

Yang

Loh

2004

BMC Cell Biol

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Background: Endopolyploidy is developmentally regulated. Presence of endopolyploidy as a result of endoreduplication has been characterized in insects, mammals and plants. The family Orchidaceae is the largest among the flowering plants. Many of the members of the orchid family are commercially micropropagated. Very little has been done to characterize the ploidy variation in different tissues of the orchid plants during development.

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“…Variations in the extent of endopolyploidy during the growth of shoots have been reported in Raphanus sativus (Kudo and Kimura 2002), Allium fistulosum (Kudo et al 2003), Brassica oleracea (Kudo and Kimura 2001), several species of legume (Lagunes-Espinoza et al 2000), Lycopersicon esculentum (Smulders et al 1994), Arabidopsis thaliana (Galbraith et al 1991), and Cucumis sativus (Gilissen et al 1993). In L. esculentum, continuous flow cytometric analysis during seed germination and seedling development demonstrated endopolyploidy with a nuclear DNA content of 128C in aged leaves and petioles (Smulders et al 1994).…”

Section: Introductionmentioning

confidence: 96%

Variations in endopolyploidy level during the short period of the early growing stage in the roots and leaves of maize (Zea mays) seedlings

Ogawa

Taguchi

Miyoshi

2010

Plant Biotechnol Rep

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We used a flow cytometer to investigate the variations in endopolyploidy (the frequencies of nuclei with DNA contents equivalent to 4C through 16C) during the short period of the early growing stage in vigorously growing young tissues of maize seedlings. We examined different portions of the root and leaves that had been growing for 7 (day 7) and 13 (day 13) days after germination. Endoreplication showed two opposing phenomena without aging. In one case, the endopolyploidy of the first leaf was higher on day 13 than on day 7. In the latter case, endopolyploidy decreased, as clearly revealed by a comparison of the endopolyploidy of the second leaves and the 160-170 mm portion of the seminal root on days 7 and 13. Endopolyploidy was also lower in the top of the leaf. In roots, endopolyploidy was increased by the exogenous application of abscisic acid for only 1 day. The levels of endopolyploidy increased without an increase in cell size in the roots. These results showed that endoreplication occurs in actively growing and young tissue and that the variation can be induced in the short period examined.

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Endoreduplication Occurs during Pod Wall Development in Temperate Grain Legumes

Cited by 9 publications

References 26 publications

Unique Tissue‐Specific Cell Cycle in Physcomitrella

Unique Tissue‐Specific Cell Cycle in Physcomitrella

Systemic endopolyploidy in Spathoglottis plicata (Orchidaceae) development

Variations in endopolyploidy level during the short period of the early growing stage in the roots and leaves of maize (Zea mays) seedlings

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