Endoscopic in vivo evaluation of tissue atypia in the esophagus using a newly designed integrated endocytoscope: a pilot trial

Inoue, Haruhiro; Sasajima, Keita; Kaga, Makoto; Sugaya, Satoshi; Sato, Yoshitaka; Wada, Yoshiki; Inui, Masayuki; Satodate, Hitoshi; Kudo, Shin‐ei; Kimura, Shiro; Hamatani, Shigeharu; Shiokawa, Akira

doi:10.1055/s-2006-944667

Cited by 133 publications

(108 citation statements)

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“…In addition, the IPCL is commonly observed in squamous cell carcinoma derived from the squamous epithelium with little or no glandular structure (33,34). So, magnifying endoscopes can be used to observe the IPCL of esophageal squamous cell carcinoma but not esophageal adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Fujinon intelligent color enhancement for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesion

Shen²,

Yu³

et al. 2020

Turk J Gastroenterol

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Background/Aims: Esophageal squamous cell carcinoma is a common malignant tumor in recent years, and the key for improving the survival rate is early diagnosis and treatment. Computed virtual chromoendoscopy with the Fujinon intelligent color enhancement (FICE) system was reported to improve visualization of neoplastic and nonneoplastic lesions in gastroscopy and colonoscopy. The purpose of this study was to evaluate the value of FICE in the diagnosis of early esophageal squamous cell carcinoma and precancerous lesions. Materials and Methods: Two hundred fifty-seven patients with suspicious lesions of the esophagus were examined successively by FICE, magnifying FICE, Lugol chromoendoscopy, and magnifying Lugol chromoendoscopy in the hospital. The lesions and the intrapapillary capillary loop (IPCL, microvessels at the surface of esophageal carcinoma) were observed and compared with the pathologic diagnosis that was regarded as the golden standard. Results: The positive rates of early esophageal squamous cell carcinoma were 92.6% and 88.9% as examined by FICE and Lugol chromoendoscopy (p>0.05), and 96.3% and 92.6% as examined by magnifying FICE and magnifying Lugol chromoendoscopy (p>0.05), respectively. The magnifying FICE could observe the IPCL of the esophagus clearly. Early esophageal squamous cell carcinoma and high-grade intraepithelial neoplasia were mainly type IV and type V. Low-grade intraepithelial neoplasia and esophagitis were type II and type III, and normal esophagus was type I; however, the observation of the IPCL by magnifying Lugol chromoendoscopy was not clear. Conclusion: Fujinon intelligent color enhancement and magnifying FICE are complements to Lugol chromoendoscopy and magnifying Lugol chromoendoscopy in the diagnosis of early esophageal lesions.

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Section: Discussionmentioning

confidence: 99%

Fujinon intelligent color enhancement for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesion

Shen²,

Yu³

et al. 2020

Turk J Gastroenterol

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“…ECS is a revolutionized endoscopic imaging technique aiming to replace the histological examination of biopsy specimens, making "optical biopsy" possible while facilitating real time decision-making [8] . ECS after double CM staining using modern integrated type endocytoscopes enables in vivo visualization of living cells and evaluation of tissue atypia by approximating the tip of the endoscope onto the mucosal surface [10] . cytoscope was used, endocytoscopic evaluation of longsegment Barrett's esophagus in the present study was performed by a dual CCD integrated endocytoscope [2] .…”

Section: Discussionmentioning

confidence: 99%

“…The video processor (prototype, Olympus CV-260X) with a light source (Olympus CLV-260) allows narrow-band imaging (NBI) [2] . Methylene blue or toluidine blue single staining was initially used for endocytoscopic evaluation of esophageal lesions [6,10,11] . Recently, however double staining with a mixture of 0.05% crystal violet and 0.1% methylene blue (CM) has been also proposed during ECS [2,3] .…”

Section: Introductionmentioning

confidence: 99%

Endocytoscopic visualization of squamous cell islands within Barrett’s epithelium

Eleftheriadis¹

2013

WJGE

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AIM:To study the endocytoscopic visualization of squamous cell islands within Barrett's epithelium. METHODS:Endocytoscopy (ECS) has been studied in the surveillance of Barrett's esophagus, with controversial results. In initial studies, however, a soft catheter type endocytoscope was used, while only methylene blue dye was used for the staining of Barrett's mucosa. Integrated type endocytoscopes (GIF-Q260 EC, Olympus Corp, Tokyo, Japan) have been recently developed, with the incorporation of a high-power magnifying endocytoscope into a standard endoscope together with narrow-band imaging (NBI). Moreover, double staining with a mixture of 0.05% crystal violet and 0.1% of methylene blue (CM) during ECS enables higher quality images comparable to conventional hematoxylin eosin histopathological images. RESULTS:In vivo endocytoscopic visualization of papillary squamous cell islands within glandular Barrett's epithelium in a patient with long-segment Barrett's esophagus is reported. Conventional white light endoscopy showed typical long-segment Barrett's esophagus, with small squamous cell islands within normal Barrett's mucosa, which were better visualized by NBI endoscopy. ECS after double CM staining showed regular Barrett's esophagus, while higher magnification (× 480) revealed the orifices of glandular structures better. Furthermore, typical squamous cell papillary protrusion, classified as endocytoscopic atypia classification (ECA) 2 according to ECA, was identified within regular glandular Barrett's mucosa. Histological examination of biopsies taken from the same area showed squamous epithelium within glandular Barrett's mucosa, corresponding well to endocytoscopic findings. CONCLUSION:To our knowledge, this is the first report of in vivo visualization of esophageal papillary squamous cell islands surrounded by glandular Barrett's epithelium.© 2013 Baishideng. All rights reserved.Key words: Endocytoscopy; Barrett's esophagus; Surveillance; Endocytoscopic atypia classification; Crystal violet; Methylene blue; Hematoxylin eosin stain Core tip: Endocytoscopy has been also studied in surveillance of Barrett's esophagus, with controversial results. In initial studies, however, a soft catheter type endocytoscope was used, while only methylene blue dye was used for staining of Barrett's mucosa. In the present study, in vivo endocytoscopic visualization of papillary squamous cell islands within glandular Barrett's epithelium in a patient with long-segment Barrett's esophagus is reported.

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“…Magnifying NBI endoscopy is referred to as 'optical biopsy', and a classification based on magnifying NBI for each internal organ has been proposed (17)(18)(19)(20)(21). Improvements in endoscopic imaging technology also allow clear visualization of cells and nuclei using endocytoscopy and endomicroscopy (17,22,23). These methods may allow evaluation as cytology and histology of biopsy specimens.…”

Section: Discussionmentioning

confidence: 99%

Flexible hysteroscopy with narrow band imaging (NBI) for endoscopic diagnosis of malignant endometrial lesions

et al. 2011

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Abstract. Narrow band imaging (NBI) for detection of blood vessels and microstructures on the mucosal surface is used in gastrointestinal endoscopy since it can improve qualitative diagnosis and detection of lesion. However, there are no studies on flexible hysteroscopy using NBI. We performed flexible hysteroscopy with NBI for outpatients to investigate the sensitivity and specificity of endoscopic diagnosis of malignant endometrial lesions. Of patients who attended our hospital for suspected lesions in the uterine cavity between April 2009 and May 2010, 104 subjects underwent hysteroscopy with NBI, in addition to white light. Using the pathological diagnosis as the gold-standard, we evaluated the sensitivity and specificity of NBI hysteroscopy for detecting atypical endometrial hyperplasia (AEH) or carcinoma. The results were also compared with historical data (n=209) for conventional hysteroscopy using white light only in 2008. The sensitivities were 97.2% [95% confidence interval (95% CI): 90.3-99.7%] and 82.6% (95% CI: 74.4-89.0%) for NBI hysteroscopy and conventional hysteroscopy, respectively. The 95% CIs for the two methods did not overlap and the sensitivity of lesion detection was higher with NBI hysteroscopy. Specificities were comparable, 90.6% (95% CI: 75.0-98.0%) and between the methods. NBI hysteroscopy has increased sensitivity for detection of atypical endometrial hyperplasia (AEH) or carcinoma. A comparison with historical data suggested that NBI may be useful for diagnosis of malignant endometrial lesions. As far as we are aware, this is the first evaluation of flexible hysteroscopy with NBI for diagnosis of malignant endometrial lesions.

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Endoscopic in vivo evaluation of tissue atypia in the esophagus using a newly designed integrated endocytoscope: a pilot trial

Cited by 133 publications

References 7 publications

Fujinon intelligent color enhancement for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesion

Fujinon intelligent color enhancement for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesion

Endocytoscopic visualization of squamous cell islands within Barrett’s epithelium

Flexible hysteroscopy with narrow band imaging (NBI) for endoscopic diagnosis of malignant endometrial lesions

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