Endosperm sterol phenotype and germination in wheat

Torres, J.V.; Carbonero, Pilar; Garcı́a-Olmedo, Francisco

doi:10.1016/s0031-9422(00)94419-4

Cited by 18 publications

(4 citation statements)

References 9 publications

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“…This difference was more pronounced when analyzing refined flour (endosperm), because the high content of palmitate provided by the D genome is expressed only in the endosperm. In fact, the fatty acids in FASE from other parts of the HW kernel always have a high linoleate content (45). GC-FID Analysis of FASE.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Fatty Acid Steryl Esters in Tetraploid and Hexaploid Wheats: Identification and Comparison between Chromatographic Methods

Caboni

Iafelice

Pelillo

et al. 2005

J. Agric. Food Chem.

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Fatty acid steryl esters (FASE) in whole meal of 14 genotypes of tetraploid wheats (Triticum dicocconand T. durum) and 17 genotypes of hexaploid wheats (T. spelta and T. aestivum) were analyzed using different chromatographic strategies. By both GC-FID and HPLC-ELSD, tetraploid wheats are lacking two major peaks. The amounts of FASE, calculated on the basis of the GC-FID analysis, were double in hexaploid species as compared to tetraploids (40 and 20 mg/100 g db, respectively). HPLC with ESI-MS detection enabled the identification of FASE by the characteristic fragmentations and ion-adducts of each molecule. The distribution of steryl residues was not different between the wheat species: the main class of steryl derivatives found was the beta-sitosteryl derivatives, followed by campesteryl derivatives with small amounts of stigmasteryl esters. The esterified fatty acids explain the difference between the hexaploid and tetraploid wheats. In particular, small amounts of campesteryl and beta-sitosteryl, while no trace of stigmasteryl palmitates, were found in T. durum or its hulled ancestor T. dicoccon. Steryl oleates were not detectable in T. aestivum or its hulled ancestor T. spelta, which is consistent with the filogenesis of tetraploid and hexaploid species. Both chromatographic techniques (GC and HPLC) showed that FASE are useful to discriminate between hexaploid and tetraploid wheats from both qualitative and quantitative points of view.

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Section: Resultsmentioning

confidence: 99%

Analysis of Fatty Acid Steryl Esters in Tetraploid and Hexaploid Wheats: Identification and Comparison between Chromatographic Methods

Caboni

Iafelice

Pelillo

et al. 2005

J. Agric. Food Chem.

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“…The predominant form varies, depending on plant and tissue: S is predominant in apple fruit mesocarp (3), SE is predominant in the endosperm of some wheat varieties (18), and ASG is predominant in potato tuber (4). The distribution of these sterols in plant tissues is mainly in the microsomal fraction and in the mitochondrial plus Golgi fraction (6,7).…”

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confidence: 99%

Effect of Steryl Glycosides on the Phase Transition of Dipalmitoyl Lecithin

Mudd¹,

McManus²

1980

Plant Physiol.

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The phase transition of dipalmitoyl lecithin, measured by thermal analysis, was eliminated by the plant sterol, sitosterol, and by the derivatives steryl glucoside and acylated steryl glucoside, which were isolated from soybean lipids.When digitonin was added to dipalmitoyl lecithin-sterol mixtures, in amounts equimolar to sterol, the phase transition of the phospholipid was revealed presumably because of the formation of a sterol complex. When digitonin in molar excess of sterol was added, the endothermic peak disappeared again.

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“…During germination and seedling development there is an increase in free sterol (Grunwald, 1 9 7 5~;Duperon, 1971;Kemp et al, 1967a;Geuns, 1973; which may in part result from hydrolysis of steryl ester reserves in the seed (Torres et al, 1976;Hughes & Goad, 1983). It seems that in some seeds the capacity of the endosperm or cotyledons for sterol synthesis is rather low or even absent during the initial stages of germination (Kemp et al, 19676;Baisted, 1971;Shrewry & Stobart 1974a;McKean & Nes, 1977).…”

Section: Sterol Synthesis During Seed Development Seed Germination Amentioning

confidence: 99%

“…During the early phase of embryo axis growth the sterol requirements for new membrane formation may be met by transfer of sterol from the seed store (Torres et al, 1976) although this could not be demonstrated in Pinuspinea seedlings (McKean & Nes, 1977). However, as the seedling axis develops it acquires the ability to synthesize sterol as revealed by the incorporation of [2-14C]mevalonic acid into sterol (Geuns, 1973;Shrewry & Stobart, 1974a;Fang & Baisted, 1975;McKean & Nes, 1977).…”

Section: Sterol Synthesis During Seed Development Seed Germination Amentioning

confidence: 99%

How is sterol synthesis regulated in higher plants?

Goad

1983

Biochemical Society Transactions

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548BIOCHEMICAL SOCIETY TRANSACTIONS dione); 1 1flOH-P (1 1/?-hydroxyprogesterone; 1 1 /?-hydroxypregn-4ene-3,20-dione); 1 I-0x0-P (1 1-oxoprogesterone; pregn-4-ene-3,11,20trione); 21-d-F (2 I-deoxycortisol; 11/?,17a-dihydroxypregn-4-ene-3,20dione); 2 1 -d-E (2 1-deoxycortisone; 17a-hydroxypregn-4-ene-3,11,20trione); 1 1-deoxycortisol (Reichstein's substance S ; cortexolone; 1 7~2 l-dihydroxypregn-4-ene-3,20-dione); DHS (dihydro substance S; dihydro-1 1-deoxycortisol; 1 7a,2 1 -dihydroxy-5/?,pregnane-3,20-dione); THS (5fltetrahydro substance S ; tetrahydro-I 1-deoxycortisol; 3/?,17a,21-trihydroxy-5/?-pregnan-20-one); DOC (1 l-deoxycorticosterone; cortexone; 2 1 -hydroxypregn-4-ene-3,2O-dione); TH-DOC (tetrahydro-1 1-deoxycorticosterone; 342 l-dihydroxy-5/?-pregnan-20one); aldosterone (1 lp,2 l-dihydroxy-pregn-4-ene-3,18,2O-trione); 2a-Me,9a-FI-F (9a-fluoro-2a-methyl-1 I/?, 17a,21-trihydroxypregn-4ene-3,20-dione); glycyrrhetinic acid (glycyrrhetic acid; 3Fhydroxy-1 I-oxo-olean-12-en-30-oic acid); brassinolide [2 a,3 a,(ZZR)-(23R)-tetra hydroxy-(24S)-methyl-B-homo-7-oxa-5a-cholestan-6-onel.

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Endosperm sterol phenotype and germination in wheat

Cited by 18 publications

References 9 publications

Analysis of Fatty Acid Steryl Esters in Tetraploid and Hexaploid Wheats: Identification and Comparison between Chromatographic Methods

Analysis of Fatty Acid Steryl Esters in Tetraploid and Hexaploid Wheats: Identification and Comparison between Chromatographic Methods

Effect of Steryl Glycosides on the Phase Transition of Dipalmitoyl Lecithin

How is sterol synthesis regulated in higher plants?

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