Endothelial cell responses towards low-fouling surfaces bearing RGD in a three-dimensional environment

Sabra, Georges; Vermette, Patrick

doi:10.1016/j.yexcr.2011.05.029

Cited by 12 publications

(15 citation statements)

References 47 publications

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“…The selective interaction between compound 2 and integrins was proved by immunostaining of vinculin and cytoskeletal actin after incubation of the HUVEC in the absence or the presence of compound 2 ( Figure 2C,D), since the interaction of the integrin with its ligand will modify the cytoskeletal network. 19 The colocalization of actin fibers and vinculin demonstrated the specific interaction between compound 2 and cellular integrins. Then the HUVEC were exposed to either free biotin as a negative control or to increasing concentrations (2.5, 5, 10 μM) of compound 2 for assessing the recognition of this modified cRGDfK peptide by the integrins expressed by the treated cells.…”

Section: ■ Resultsmentioning

confidence: 97%

Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering

et al. 2012

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To control the selective adhesion of human endothelial cells and human serum proteins to bioceramics of different compositions, a multifunctional ligand containing a cyclic arginine-glycine-aspartate (RGD) peptide, a tetraethylene glycol spacer, and a gallate moiety was designed, synthesized, and characterized. The binding of this ligand to alumina-based, hydroxyapatite-based, and calcium phosphate-based bioceramics was demonstrated. The conjugation of this ligand to the bioceramics induced a decrease in the nonselective and integrin-selective binding of human serum proteins, whereas the binding and adhesion of human endothelial cells was enhanced, dependent on the particular bioceramics.

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Section: ■ Resultsmentioning

confidence: 97%

Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering

et al. 2012

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“…We found that approximately 60% of the cells did not survive the plasmin-induced fibrin degradation. 12 Given that cells proliferated under the different culture conditions, insulin secretion could not be normalized to the initial cell seeding density.…”

Section: Glucose-stimulated Insulin Secretion (Gsis)mentioning

confidence: 99%

“…Another method to quantify these 3D systems is to enzymatically degrade the matrix to reveal protein expression. This method is also flawed as these enzymes also affect cells within, causing cell damage and even cell death, thus preventing an accurate quantification of the culture …”

Section: Introductionmentioning

confidence: 99%

“…This method is also flawed as these enzymes also affect cells within, causing cell damage and even cell death, thus preventing an accurate quantification of the culture. 12 One promising way to circumvent the problems outlined above is to use a perfusion bioreactor. Perfusion bioreactors allow manipulation of physiological parameters such as pH, dissolved oxygen concentration, temperature, and flow thus exposing cells to mechanical stimuli, faster nutrient and oxygen delivery, and quicker removal of waste materials which might impact cell viability and function.…”

Section: Introductionmentioning

confidence: 99%

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An In‐situ glucose‐stimulated insulin secretion assay under perfusion bioreactor conditions

Sharp

Vermette

2016

Biotechnology Progress

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Perfusion bioreactors, unlike traditional in vitro cell culture systems, offer stringent control of physiological parameters such as pH, flow, temperature, and dissolved oxygen concentration which have been shown to have an impact on cellular behaviour and viability. Due to the relative infancy and the growing interest in these in vitro culture systems, detection methods to monitor cell function under dynamic perfusion bioreactor conditions remains one of the main challenges. In this study, INS-1 cells, a cell line which exhibit glucose-stimulated insulin secretion, were embedded in fibrin and cultured under perfusion bioreactor conditions for 48 h and then exposed to either a high-, or low-glucose concentration for 24 h. These cultures were compared to non-bioreacted controls. Bioreacted cultures exposed to a high-glucose concentration showed the highest glucose-stimulated insulin secretion when compared to those in a low-glucose environment. The stimulation index, a marker for insulin secretion functionality, increased over time. A lower incidence of apoptotic cells was observed in the bioreacted cultures when compared to non-bioreacted ones, as evaluated by a TUNEL assay. Immunofluorescence staining of Ki67 and insulin was performed and showed no differences in the incidence of proliferative cells between conditions (bioreacted and non-bioreacted), where all cells stained positive for insulin. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:454-462, 2017.

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“…Therefore, study done under serum‐free conditions cannot accurately reflect cell‐specific interactions with surfaces under normal culture conditions. To address this issue, protein‐resistant polymers have been used as spacers, and further conjugated with cell‐specific binding ligands or cell‐adhesive proteins to promote cell‐specific binding in complex culture media 28–30. However, most of the surfaces used were planar, and the influence of the surface micro/nano‐structures on cell‐specific binding and the ensuing cellular responses was overlooked.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Specific Binding of L929 Fibroblasts: Effects of Multi‐Scale Topography of GRGDY Peptide Modified Surfaces

Zhou

et al. 2012

Macromolecular Bioscience

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Surface chemical composition and roughness caused by micro/nano-topographical structures are two predominant factors in determining cellular response. A series of gold nanoparticle layers (GNPLs) with increasing surface roughness are prepared by depositing gold nanoparticles onto planar gold films. Glycine-arginine-glycine-aspartic acid-tyrosine (GRGDY) peptide is then conjugated to the surface through poly[oligo(ethylene glycol) methacrylate] polymer brushes as spacers. The pristine micro/nano-roughness structures significantly inhibit L929 cell growth. After modification with POEGMA-GRGDY, however, the micro/nano-structures greatly enhance L929 cell-specific interactions while maintaining superior low-fouling ability compared to planar gold.

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Endothelial cell responses towards low-fouling surfaces bearing RGD in a three-dimensional environment

Cited by 12 publications

References 47 publications

Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering

Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering

An In‐situ glucose‐stimulated insulin secretion assay under perfusion bioreactor conditions

Enhancing Specific Binding of L929 Fibroblasts: Effects of Multi‐Scale Topography of GRGDY Peptide Modified Surfaces

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