Endothelial Cell Seeding on Different Polyurethanes

Gerlach, Jörg C.; Schauwecker, H. H.; Hennig, Ewald M.; Bücherl, E. S.

doi:10.1111/j.1525-1594.1989.tb02849.x

Cited by 17 publications

(5 citation statements)

References 7 publications

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“…Pre-coating with collagen onto gelseal has been demonstrated to be superior to initially pre-coating onto gelseal or ePTFE (GERLACH et al, 1989;VOHRA et al, 1991). Combinations of FN/collagen or laminin/collagen have been shown significantly to enhance both ECA and ECR rather than FN, laminin and/or fibrin glue alone (HAEGERSTRAND et al, 1993;KAEHLER et al, 1989).…”

Section: Chemical Coatingsmentioning

confidence: 97%

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Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Salacinski

Tiwari

Hamilton

et al. 2001

Med. Biol. Eng. Comput.

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Surgical treatment of vascular disease has become common. The use of synthetic materials is limited to grafts larger than 5-6mm, because of the frequency of occlusion observed with small-diameter prosthetics. An alternative would be a hybrid or tissue-engineered graft with the surface coated with a monolayer of the patient's own cells. Currently, to be effective, high-density seeding regimens have to be undertaken. This is because endothelial cells (ECs) are washed off the graft lumen once exposed to physiological blood flow. EC attachment has been shown to be significantly improved by pre-coating with substances known to attach ECs selectively. The review examines the various types of coating and bonding technology used to date to enhance endothelial cell attachment onto the surface of prosthetic vascular bypass grafts.

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Section: Chemical Coatingsmentioning

confidence: 97%

“…This is thought to be dependent on the surface characteristics of the polymeric material. With polyurethanes, an incubation time of 45 min is thought to be optimum (GERLACH et al, 1989) in some types, but, in others, can range from 1-24 h .…”

Section: Incubation Period"mentioning

confidence: 98%

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Salacinski

Tiwari

Hamilton

et al. 2001

Med. Biol. Eng. Comput.

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“…The majority of work on promoting endothelial cell adherence and growth to permanent nonbiodegradable polymers has involved the modification of the surface with single coatings of endothelial‐specific adhesion proteins. These substances include albumin (112), albumin‐heparin conjugates (113,114), collagen (115–124), collagen‐elastin matrices (125), fibronectin (42,47,117,118,126–134), gelatin (26,117,122,129,135), laminin (76,121,136–140), ECM (141), dipyridamole (142), peptide fragments (143), and preclotting with blood, plasma, fibrin glue (144), and serum (85,124,131,139,145,146). RGD enriched fibrin glue is the principal substrate used clinically in the largest trials of two‐stage seeding in humans (25).…”

Section: Methodsmentioning

confidence: 99%

Improving the Clinical Patency of Prosthetic Vascular and Coronary Bypass Grafts: The Role of Seeding and Tissue Engineering

et al. 2002

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In patients requiring coronary or peripheral vascular bypass procedures, autogenous vein is currently the conduit of choice. If this is unavailable, then a prosthetic material is used. Prosthetic graft is liable to fail due to occlusion of the graft. To prevent graft occlusion, seeding of the graft lumen with endothelial cells is undertaken. Recent advances have also looked at developing a completely artificial biological graft engineered from the patient's cells with properties similar to autogenous vessels. This review encompasses the developments in the two principal technologies used in developing hybrid coronary and peripheral vascular bypass grafts, that is, seeding and tissue engineering.

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“…In the novel bioreactor construction, hepatocyte immobilization is achieved via cell attachment supporting capillaries 69 and use of a cell compatible capillary potting material. 70 One specific feature of the model is that the woven capillaries enter and leave the reactor in sorted, discrete bundles so that they can serve different functions. Independent plasma inflow, plasma outflow and oxygen supply/carbon dioxide removal are therefore possible.…”

Section: Novel Reactor Developmentmentioning

confidence: 99%

Bioreactors for Hybrid Liver Support: Historical Aspects and Novel Designs

Busse

Gerlach

1999

Annals of the New York Academy of Sciences

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A novel bioreactor construction has been designed for the utilization of hepatocytes and sinusoidal endothelial cells. The reactor is based on capillaries for hepatocyte aggregate immobilization. Three separate capillary membrane systems, each permitting a different function are woven in order to create a three dimensional network. Cells are perfused via independent capillary membrane compartments. Decentralized oxygen supply and carbon dioxide removal with low gradients are possible. The use of identical parallel units to supply hepatocytes facilitates scale up. In vitro studies demonstrate long-term external metabolic function in primary isolated hepatocytes within bioreactors. These systems are capable of supporting essential liver functions. Animal experiments have verified the possibility of scaling-up the bioreactors for clinical treatment. However, since there is no reliable animal model for investigation of the treatment of acute liver failure, the promising results obtained from these studies have limited relevance. The small number of clinical studies performed so far is not sufficient to reach conclusions about improvements in the therapy of acute liver failure. Although important progress has been made in the development of these systems, various hepatocyte culture models and bioreactor constructions are being discussed in the literature, which indicates competition in this field of medical research. An overview, which emphasizes the development of hepatocyte culture models for bioreactors, subsequent in vitro studies, animal studies, and clinical application, is also provided.

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Endothelial Cell Seeding on Different Polyurethanes

Cited by 17 publications

References 7 publications

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

Improving the Clinical Patency of Prosthetic Vascular and Coronary Bypass Grafts: The Role of Seeding and Tissue Engineering

Bioreactors for Hybrid Liver Support: Historical Aspects and Novel Designs

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