Endothelial damage of a donor cornea depending on the donor insertion method during Descemet-stripping automated endothelial keratoplasty in porcine eyes

Hwang, Hosik; Kim, Man Soo

doi:10.1007/s10384-009-0713-4

Cited by 11 publications

(3 citation statements)

References 12 publications

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“…The pig experimental model has also been used in eye banking, tissue engineering, 8 and pharmacology studies 9,10 . Several corneal surgeries have been developed using the ex vivo pig model, including radial keratotomy, thermokeratoplasty, photorefractive keratectomy, LASIK, 11 Intacs, keratophakia, keratoprosthesis, anterior lamellar keratoplasty, endothelial keratoplasties, femtosecond assisted keratoplasty, and penetrating keratoplasty 12,13 . The pig eye also remains the preferred model for the surgical training of ophthalmology residents 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of the pig and feline models for full thickness corneal transplantation

Brunette

Rosolen

Carrier

et al. 2011

Veterinary Ophthalmology

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Section: Introductionmentioning

confidence: 99%

Comparison of the pig and feline models for full thickness corneal transplantation

Brunette

Rosolen

Carrier

et al. 2011

Veterinary Ophthalmology

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“…An experimental study on porcine eyes showed no significant quantitative difference in ECL between taco, drag using forceps, or drag with suture, but each had a different specific pattern of endothelial damage. [ 8 ]…”

Section: Discussionmentioning

confidence: 99%

The outcome of 70/30 taco insertion through a 2.8 mm clear corneal incision in Descemet’s stripping automated endothelial keratoplasty - A retrospective analysis

Narayanan

Jain²,

Ravi³

et al. 2021

Indian Journal of Ophthalmology

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Purpose: To assess the long-term outcome of graft insertion by taco technique through a 2.8-mm clear corneal incision in patients undergoing Descemet’s stripping automated endothelial keratoplasty (DSAEK). Methods: This is a retrospective interventional case series of 77 eyes of 75 patients who underwent DSAEK in a tertiary eye hospital. The DSAEK donor grafts were folded to an uneven 70/30 taco and held at a single point using Utrata forceps. All insertions were through a 2.8-mm clear corneal incision except the two aphakic patients requiring combined SFIOL implantation. All patients underwent a comprehensive eye examination preoperatively and were followed up to 6 years postoperatively. Visual outcomes, graft clarity, and complications of all and endothelial cell loss in 22 patients with available postop specular microscopy were analyzed. Results: Overall, 59 (76.6%) had clear grafts until the final follow-up. Visual acuity improved in 48 (62.3%) from an average of 1.3 to 0.8 logMAR ( P = 0.0001). Vision was maintained in seven and worsened in four eyes. Grafts failed in 18 (23.3%) eyes: seven (9%) were primary failures, two post rejection, four done for failed PK did not clear, four due to worsening of preexisting glaucoma, and one noncompliant failed eventually. Average endothelial cell density reduction was 26.3% (mean preop donor 2419 to postop 1779 cells/mm 2 ; P = 0.000). Conclusion: Our study shows good long-term clinical outcome of DSAEK using Taco technique through a 2.8-mm clear corneal incision in a tertiary hospital.

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“…Terry, Hoar, Wall and Ousley (2006) used SEM to compare two different corneal endothelial transplantation techniques. Still within the scope of the transplant, Hwang and Kim (2009) again opted for the use of SEM to analyse the damage caused to the corneal endothelium cells in DSAEK surgery. Villarrubia et al (2017) also chose SEM with a 100-fold magnification to analyse the stromal receptor bed from three different methods to obtain the donor button.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

Albuquerque

Pigatto

2020

Semina: Ciênc. Agrár.

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The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.

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Endothelial damage of a donor cornea depending on the donor insertion method during Descemet-stripping automated endothelial keratoplasty in porcine eyes

Abstract: The amount of endothelial damage did not differ significantly among the three different methods of donor insertion during DSAEK in this animal model, but each method exhibited a different endothelial damage pattern.

Cited by 11 publications

References 12 publications

Comparison of the pig and feline models for full thickness corneal transplantation

Comparison of the pig and feline models for full thickness corneal transplantation

The outcome of 70/30 taco insertion through a 2.8 mm clear corneal incision in Descemet’s stripping automated endothelial keratoplasty - A retrospective analysis

Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

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