Endothelial extracellular vesicles promote tumour growth by tumour‐associated macrophage reprogramming

Njock, Makon‐Sébastien; O’Grady, Tina; Nivelles, Olivier; Lion, Michelle; Jacques, Sophie; Cambier, Maureen; Herkenne, Stéphanie; Müller, Florian; Christian, Aurélie; Remacle, Claire; Guiot, Julien; Rahmouni, Souad; Dequiedt, Samuel; Struman, Ingrid

doi:10.1002/jev2.12228

Cited by 31 publications

(18 citation statements)

References 38 publications

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“…Idiopathic pulmonary fibrosis (IPF) is the archetypal progressive fibrosing interstitial lung disease, of unknown etiology and cure which leads to rapid death (2-3 years after diagnosis) [17][18][19][20][21]. IPF is characterized by progressive and irreversible destruction of the lung architecture caused by excessive extracellular matrix (ECM) deposition and remodeling, resulting in the formation of fibrotic scar that ultimately leads to organ destruction and death from respiratory failure [22,23].microRNAs (miRNAs) are small noncoding RNA molecules (20-22 nucleotides) that post-transcriptionally modulate gene expression by blocking the translation or inducing degradation of target mRNAs [24][25][26][27][28]. Several studies reported the dysregulation of the levels of circulating miRNAs in lung diseases [29,30].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we identified a unique signature of three sputum-derived miRNAs presenting an aberrant expression in IPF patients compared to healthy donors [29]. Besides their capacity as potential biomarkers of lung diseases, miRNAs are essential regulators of various cellular processes, including fibrosis [27,[31][32][33]. Several studies have shown that miRNAs also participate in SARS-CoV-2 infection and pathogenesis through different mechanisms [34,35], such as: host cell miRNA expression interfering with SARS-CoV-2 cell entry [36,37]; SARS-CoV-2-derived RNA transcripts acting as competitive endogenous RNAs that may attenuate host cell miRNA expression [38][39][40][41]; and host cell miRNA expression modulating SARS-CoV-2 replication [38,[42][43][44].…”

Section: Introductionmentioning

confidence: 99%

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Systematic review of overlapping microRNA patterns in COVID-19 and idiopathic pulmonary fibrosis

et al. 2023

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Background Pulmonary fibrosis is an emerging complication of SARS-CoV-2 infection. In this study, we speculate that patients with COVID-19 and idiopathic pulmonary fibrosis (IPF) may share aberrant expressed microRNAs (miRNAs) associated to the progression of lung fibrosis. Objective To identify miRNAs presenting similar alteration in COVID-19 and IPF, and describe their impact on fibrogenesis. Methods A systematic review of the literature published between 2010 and January 2022 (PROSPERO, CRD42022341016) was conducted using the key words (COVID-19 OR SARS-CoV-2) AND (microRNA OR miRNA) or (idiopathic pulmonary fibrosis OR IPF) AND (microRNA OR miRNA) in Title/Abstract. Results Of the 1988 references considered, 70 original articles were appropriate for data extraction: 27 studies focused on miRNAs in COVID-19, and 43 on miRNAs in IPF. 34 miRNAs were overlapping in COVID-19 and IPF, 7 miRNAs presenting an upregulation (miR-19a-3p, miR-200c-3p, miR-21-5p, miR-145-5p, miR-199a-5p, miR-23b and miR-424) and 9 miRNAs a downregulation (miR-17-5p, miR-20a-5p, miR-92a-3p, miR-141-3p, miR-16-5p, miR-142-5p, miR-486-5p, miR-708-3p and miR-150-5p). Conclusion Several studies reported elevated levels of profibrotic miRNAs in COVID-19 context. In addition, the balance of antifibrotic miRNAs responsible of the modulation of fibrotic processes is impaired in COVID-19. This evidence suggests that the deregulation of fibrotic-related miRNAs participates in the development of fibrotic lesions in the lung of post-COVID-19 patients.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic review of overlapping microRNA patterns in COVID-19 and idiopathic pulmonary fibrosis

et al. 2023

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“…While CAFs were found to support the surrounding tumor cells by transferring tumor-supportive signals via EVs, thereby contributing to tumor growth and metastasis in several cancer types ( 62 , 66 – 68 ). In addition, endothelial cell derived-EVs were shown to affect macrophages, by converting them to a M2-like phenotype thereby inducing an immunosuppressive environment and aiding in cancer progression ( 69 ). Accordingly, TAMs-released EVs were shown to promote EMT and angiogenesis by regulating the tumor cells and endothelial cells, thereby promoting metastasis in hepatic cancer ( 70 ).…”

Section: Tumor Derived Evsmentioning

confidence: 99%

Extracellular vesicles in neuroblastoma: role in progression, resistance to therapy and diagnostics

Dhamdhere,

Spiegelman

2024

Front. Immunol.

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Neuroblastoma (NB) is the most common extracranial solid pediatric cancer, and is one of the leading causes of cancer-related deaths in children. Despite the current multi-modal treatment regimens, majority of patients with advanced-stage NBs develop therapeutic resistance and relapse, leading to poor disease outcomes. There is a large body of knowledge on pathophysiological role of small extracellular vesicles (EVs) in progression and metastasis of multiple cancer types, however, the importance of EVs in NB was until recently not well understood. Studies emerging in the last few years have demonstrated the involvement of EVs in various aspects of NB pathogenesis. In this review we summarize these recent findings and advances on the role EVs play in NB progression, such as tumor growth, metastasis and therapeutic resistance, that could be helpful for future investigations in NB EV research. We also discuss different strategies for therapeutic targeting of NB-EVs as well as utilization of NB-EVs as potential biomarkers.

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“…hsa-miR-144-3p M1 [53,54] hsa-miR-148a-3p M1 [55,56] hsa-miR-342-5p M1 [57] hsa-miR-206-3p M1 [58] hsa-miR-1-3p M2b [59] hsa-miR-122-5p M2 [60] hsa-miR-126-3p M2 [61,62] hsa-miR-142-5p M2 [63][64][65] hsa-miR-195-3p M2 [66,67] hsa-miR-22-5p M2 [68] hsa-miR-223-3p M2 [65] hsa-miR-328-5p M2 [69] hsa-miR-423-5p M2 [70] hsa-miR-451a M2 [71,72] 48 h with or without 5 µg/mL matrix vesicles (MV) harvested from MG63s grown in growth media until 24 h after confluence; relative quantification of messenger RNA (mRNA) expression of BGLAP (H), RUNX2 (I), and Osterix/SP7 (J) in MG63 cells treated for 24 or 48 h with or without 5 µg/mL matrix vesicles (MV) from the same conditions. mRNA was quantified via RT-PCR and normalized to GAPDH expression.…”

Section: Mirna M1/m2 Polarization Referencesmentioning

confidence: 99%

Osteoblast-Derived Matrix Vesicles Exhibit Exosomal Traits and a Unique Subset of microRNA: Their Caveolae-Dependent Endocytosis Results in Reduced Osteogenic Differentiation

Skelton

Cohen

Boyan

et al. 2023

IJMS

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Matrix vesicles (MVs) are nano-sized extracellular vesicles that are anchored in the extracellular matrix (ECM). In addition to playing a role in biomineralization, osteoblast-derived MVs were recently suggested to have regulatory duties. The aims of this study were to establish the characteristics of osteoblast-derived MVs in the context of extracellular vesicles like exosomes, assess their role in modulating osteoblast differentiation, and examine their mechanism of uptake. MVs were isolated from the ECM of MG63 human osteoblast-like cell cultures and characterized via enzyme activity, transmission electron microscopy, nanoparticle tracking analysis, Western blot, and small RNA sequencing. Osteoblasts were treated with MVs from two different culture conditions (growth media [GM]; osteogenic media [OM]) to evaluate their effects on the differentiation and production of inflammatory markers and on macrophage polarization. MV endocytosis was assessed using a lipophilic, fluorescent dye and confocal microscopy with the role of caveolae determined using methyl-β-cyclodextrin. MVs exhibited a four-fold enrichment in alkaline phosphatase specific activity compared to plasma membranes; were 50–150 nm in diameter; possessed exosomal markers CD63, CD81, and CD9 and endosomal markers ALIX, TSG101, and HSP70; and were selectively enriched in microRNA linked to an anti-osteogenic effect and to M2 macrophage polarization. Treatment with GM or OM MVs decreased osteoblast differentiation. Osteoblasts endocytosed MVs using a mechanism that involves caveolae. These results support the hypothesis that osteoblasts produce MVs that participate in the regulation of osteogenesis.

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Endothelial extracellular vesicles promote tumour growth by tumour‐associated macrophage reprogramming

Cited by 31 publications

References 38 publications

Systematic review of overlapping microRNA patterns in COVID-19 and idiopathic pulmonary fibrosis

Systematic review of overlapping microRNA patterns in COVID-19 and idiopathic pulmonary fibrosis

Extracellular vesicles in neuroblastoma: role in progression, resistance to therapy and diagnostics

Osteoblast-Derived Matrix Vesicles Exhibit Exosomal Traits and a Unique Subset of microRNA: Their Caveolae-Dependent Endocytosis Results in Reduced Osteogenic Differentiation

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