Endothelial progenitor cells are reduced in refractory hypertension

Oliveras, Anna; Soler, Marta; Martínez-Estrada, Ofelia M.; Vázquez, Susana; Marco-Feliu, D; Vila, Joan; Vilaró, Senén; Lloveras, J

doi:10.1038/sj.jhh.1002304

Cited by 77 publications

(39 citation statements)

References 47 publications

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“…Moreover, a recent study by Delva et al 31 examined the number of cultured early EPCs in 36 patients with essential hypertension and 24 control subjects and did not report a reduced number of cultured early EPCs in hypertensive patients. However, in patients with advanced and refractory hypertension, EPC numbers were reduced in a recent study by Oliveras et al 32 Therefore, the observations of the present study in the context of previous findings are consistent with the notion that the dysfunction of early EPCs is rather substantially more pronounced and begins earlier as compared with a detectable reduction of the number of circulating early EPCs in patients with hypertension. Importantly, the present study provides for the first time evidence that the in vivo re-endothelialization capacity of early EPCs is already profoundly reduced in patients with prehypertension and is related to impaired endothelium-dependent vasodilation and senescence of early EPCs.…”

Section: Discussionsupporting

confidence: 82%

Impaired Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Prehypertension

et al. 2010

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Abstract-Prehypertension is a highly frequent condition associated with an increased cardiovascular risk. Endothelial dysfunction is thought to promote the development of hypertension and vascular disease; however, underlying mechanisms remain to be further determined. The present study characterizes for the first time the in vivo endothelial repair capacity of early endothelial progenitor cells (EPCs) in patients with prehypertension/hypertension and examines its relation with endothelial function. Early EPCs were isolated from healthy subjects and newly diagnosed prehypertensive and hypertensive patients (nϭ52). In vivo endothelial repair capacity of EPCs was examined by transplantation into a nude mouse carotid injury model. EPC senescence was determined (RT-PCR of telomere length). NO and superoxide production of EPCs were measured using electron spin resonance spectroscopy analysis. CD34 ϩ /KDR ϩ mononuclear cells and circulating endothelial microparticles were examined by fluorescence-activated cell sorter analysis. Endothelium-dependent and -independent vasodilations were determined by high-resolution ultrasound. In vivo endothelial repair capacity of EPCs was substantially impaired in prehypertensive/hypertensive patients as compared with healthy subjects (re-endothelialized area: 15Ϯ3%/13Ϯ2% versus 28Ϯ3%; PϽ0.05 versus healthy subjects). Senescence of EPCs in prehypertension/hypertension was substantially increased, and NO production was markedly reduced. Moreover, reduced endothelial repair capacity of early EPCs was significantly related to an accelerated senescence of early EPCs and impaired endothelial function. The present study demonstrates for the first time that in vivo endothelial repair capacity of early EPCs is reduced in patients with prehypertension and hypertension, is related to EPC senescence and impaired endothelial function, and likely represents an early event in the development of hypertension. (Hypertension. 2010;55:1389-1397.)

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Section: Discussionsupporting

confidence: 82%

Impaired Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Prehypertension

et al. 2010

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“…Meanwhile, hypertension has an increasingly intensified damage to EPCs with the increase of blood pressure and the development of hypertension pathogenetic process. [5] This research showed that, in comparison with the control group, the proliferation ability of peripheral blood EPCs was obviously decreased in the hypertension group, which conformed to previous studies (p < .01). [5] Furthermore, authors of this article found that, in comparison with the control group, EPCs migration and adhesion ability were also obviously decreased in the hypertension group.…”

Section: Discussionsupporting

confidence: 78%

“…[5] This research showed that, in comparison with the control group, the proliferation ability of peripheral blood EPCs was obviously decreased in the hypertension group, which conformed to previous studies (p < .01). [5] Furthermore, authors of this article found that, in comparison with the control group, EPCs migration and adhesion ability were also obviously decreased in the hypertension group. The results suggested that, not only the number of peripheral blood EPCs, but also their migration and adhesion ability were degraded under the condition of hypertension.…”

Section: Discussionsupporting

confidence: 78%

Effects of benner pury on the function of endothelial progenitor cells in vitro from patients with hypertension in a time-dependent manner

Wen¹,

Lian²,

Alatan³

2016

DCC

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Objective: To investigate the effect of Benner Pury on Endothelial progenitor cells (EPCs) proliferation, migration, adhesion, apoptosis and oxidative stress in vitro from patients with hypertension and its time dependence. Methods: The patients who were diagnosed as hypertension grade 1 according to the standard "Guidelines for Prevention and Treatment of Hypertension" were enrolled. Mononuclear cells were isolated by density gradient centrifugation and stained with fluorescence chemical acetylated low density lipoprotein marked with DiI (acLDL-DiI) and fluorescein isothiocyanate (FITC)-lectin. Double staining positive cells were considered as the differentiation of EPCs. The control group included healthy subjects matched with study group in age, gender. EPCs cultivated for 5 days were used for study. Cells harvested at different set times under the stimulation of Benner Pury, and the EPCs proliferation, migration, adhesion ability, apoptosis and oxidative stress indicators were detected respectively. Results: (1) Compared with the control group, peripheral blood EPCs proliferation, migration and adhesion ability were obviously decreased in the hypertension group (p < .01), EPCs apoptosis rate and oxidative stress response were significantly increased (p < .01). (2) Benner Pury significantly increased the EPCs proliferation, migration, adhesion ability and improved apoptosis and oxidative stress reaction in a time-dependent manner. Conclusions: Benner Pury can improve the EPCs proliferation, migration, adhesion, apoptosis and oxidative stress in patients with hypertension in a time-dependent manner.Key Words: Hypertension, Endothelial progenitor cells, Proliferation, Migration, Adhesion, Apoptosis, Oxidative stress Endothelial progenitor cells (EPCs), which are precursor cells of vascular endothelial cells, can be involved in angiogenesis, vasculogenesis and the repair of endothelial injury, and are closely associated with hypertension and target organ damage caused by hypertension.[1] Angiotensin II plays an important role in the occurrence and the development of hypertension. Angiotensin converting enzyme inhibitors (ACEIs) quell the activity of Angiotensin II by inhibiting angiotensin converting enzymes, and then lower blood pressure. This study used EPCs in vitro from patients with hypertension under the intervention of Benner Pury classified as ACEI drugs, on one hand, to observe the changes in EPCs proliferation, migration, adhesion, apoptosis and oxidative stress at different set times, on the other hand, to investigate the effect of ACEI anti-hypertensive drugs on the function of peripheral circulating EPCs in patients with hypertension and further explore whether therapeutical effects of ACEI on patients with hypertension were related to the improve-

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“…Further, it has also been suggested that differentiation potential of bone-marrow-derived MSCs decreases with age [11]. Recent data also indicate that EPC function is diminished in patients with severe vascular disease and multiple coronary risk factors [12,13]. …”

mentioning

confidence: 99%

Endothelial Differentiation of Adipose-Derived Stem Cells from Elderly Patients with Cardiovascular Disease

Zhang

Moudgill²,

Hager³

et al. 2011

Stem Cells and Development

101

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Adipose-derived stem cells (ASCs) possess significant therapeutic potential for tissue engineering and regeneration. This study investigates the endothelial differentiation and functional capacity of ASCs isolated from elderly patients. Isolation of ASCs from 53 patients (50-89 years) revealed that advanced age or comorbidity did not negatively impact stem cell harvest; rather, higher numbers were observed in older donors (>70 years) than in younger. ASCs cultured in endothelial growth medium-2 for up to 3 weeks formed cords upon Matrigel and demonstrated acetylated-low-density lipoprotein and lectin uptake. Further stimulation with vascular endothelial growth factor and shear stress upregulated endothelial cell-specific markers (CD31, von Willebrand factor, endothelial nitric oxide synthase, and VE-cadherin). Inhibition of the PI 3 K but not mitogen-activated protein kinase pathway blocked the observed endothelial differentiation. Shear stress promoted an antithrombogenic phenotype as demonstrated by production of tissue-plasminogen activator and nitric oxide, and inhibition of plasminogen activator inhibitor-1. Shear stress augmented integrin a 5 b 1 expression and subsequently increased attachment of differentiated ASCs to basement membrane components. Finally, ASCs seeded onto a decellularized vein graft resisted detachment despite application of shear force up to 9 dynes. These results suggest that (1) advanced age and comorbidity do not negatively impact isolation of ASCs, and (2) these stem cells retain significant capacity to acquire key endothelial cell traits throughout life. As such, adipose tissue is a practical source of autologous stem cells for vascular tissue engineering. IntroductionU se of adult stem cells for vascular tissue engineering and regeneration continues to gain momentum as research reveals their improved potency and function. The majority of work involves mesenchymal stem cells (MSCs) derived from bone marrow aspiration and endothelial progenitor cells (EPCs) obtained from blood. Each of these cell types have been used to line vascular scaffolds in the creation of a tissue engineered bypass graft [1][2][3], as well as in various strategies to promote therapeutic angiogenesis in the coronary and peripheral circulations [4][5][6][7]. Although these cells are appropriate for vascular tissue engineering, their availability in patients most likely to benefit from this technology raises practical concerns. The number of stem and progenitor cells derived from bone marrow and blood decrease significantly with age and patient comorbidity [8][9][10]. Further, it has also been suggested that differentiation potential of bone-marrow-derived MSCs decreases with age [11]. Recent data also indicate that EPC function is diminished in patients with severe vascular disease and multiple coronary risk factors [12,13].An alternative source for autologous adult stem cells is adipose tissue. Adipose-derived stem cells (ASCs) are multipotent, with the capacity to differentiate into adipocytes, chondrocytes,...

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Endothelial progenitor cells are reduced in refractory hypertension

Cited by 77 publications

References 47 publications

Impaired Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Prehypertension

Impaired Endothelial Repair Capacity of Early Endothelial Progenitor Cells in Prehypertension

Effects of benner pury on the function of endothelial progenitor cells in vitro from patients with hypertension in a time-dependent manner

Endothelial Differentiation of Adipose-Derived Stem Cells from Elderly Patients with Cardiovascular Disease

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