Endothelial Recovery

Losordo, Douglas W.; Isner, Jeffrey M.; Díaz‐Sandoval, Larry J.

doi:10.1161/01.cir.0000071083.31270.c3

Cited by 78 publications

(14 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Clinical observations and animal studies have led investigators to test the hypothesis that enhancing re-endothelialization might inhibit neointimal formation following vascular injury. Indeed, early restoration of endothelial integrity through local VEGF administration was associated with reduced proliferation of intimal vascular smooth muscle cells, providing indirect evidence to support a role of endothelial integrity in suppressing intimal hyperplasia [11–13]. …”

mentioning

confidence: 99%

Metabolic and Structural Integrity of Magnetic Nanoparticle-Loaded Primary Endothelial Cells for Targeted Cell Therapy

Orynbayeva

Sensenig

Polyak

2015

Nanomedicine (Lond.)

View full text Add to dashboard Cite

Aim To successfully translate magnetically mediated cell targeting from bench to bedside, there is a need to systematically assess the potential adverse effects of magnetic nanoparticles (MNPs) interacting with ‘therapeutic’ cells. Here, we examined in detail the effects of internalized polymeric MNPs on primary rat endothelial cells’ structural intactness, metabolic integrity and proliferation potential. Materials & methods The intactness of cytoskeleton and organelles was studied by fluorescent confocal microscopy, flow cytometry and high-resolution respirometry. Results MNP-loaded primary endothelial cells preserve intact cytoskeleton and organelles, maintain normal rate of proliferation, calcium signaling and mitochondria energy metabolism. Conclusion This study provides supportive evidence that MNPs at doses necessary for targeting did not induce significant adverse effects on structural integrity and functionality of primary endothelial cells – potential cell therapy vectors.

show abstract

mentioning

confidence: 99%

Metabolic and Structural Integrity of Magnetic Nanoparticle-Loaded Primary Endothelial Cells for Targeted Cell Therapy

Orynbayeva

Sensenig

Polyak

2015

Nanomedicine (Lond.)

View full text Add to dashboard Cite

show abstract

“…A delayed re-endothelialization keeps these mechanisms and critically contributes to neontimal thickening. [12].…”

Section: The Pathophysiology Of Arterial Rest-enosis After Angioplastymentioning

confidence: 99%

“…For instance, many efforts have been directed to obtain a rapid re-endothelialization, fundamental to regulate permeability, thrombogenicity, leukocyte adherence, as well as production of growth factor [28].…”

Section: Current Approaches To Prevent Resteno-sismentioning

confidence: 99%

Porphyrin-Photosensitized Processes: Their Applications in the Prevention of Arterial Restenosis

Magaraggia

Marigo²,

Pagnan³

et al. 2007

CHAMC

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is based on the use of a photozensitising compound which is accumulated by rapidly proliferating cells. Subsequent irradiation with light wavelengths specifically absorbed by the photosensitiser promotes the generation of reactive short-lived oxygen species which cause an irreversible and selective damage. Endovascular interventions to correct obstructive arterial disease have been developed worldwide with excellent short term results. However, long term patency is still limited by the onset of restenosis, due to subsequent intimal hyperplasia (IH). IH is characterized by proliferation and migration of smooth muscle cells (SMC) and extracellular matrix production. Targeting of SMC by photozensitisers can be efficiently achieved by taking advantage of the receptors for low density lipoproteins (LDL) expressed by such cells. Thus, preference is given to hydrophobic compounds which readily partition in the lipid matrix of LDL. We developed a liposomal formulation of a highly hydrophobic photozensitising agent, Zn(II)-phthalocyanine (ZnPc). The liposome-delivered ZnPc was readily taken up by cultured SMC cells and preferentially localized in the Golgi apparatus. Red light irradiation of incubated SMC induced cell death. Extension of these investigations to an in vivo rabbit model showed that ZnPc mainly accumulated in the media layer, where PDT induces the main damage through cellular depletion due to apoptosis of SMC, changes in the extracellular matrix with generation of a barrier to cellular migration, and acceleration of re-endothelization. Initial clinical applications showed that PDT safely and effectively prevents restenosis after angioplasty up to a 6 month follow-up.

show abstract

“…Until the endothelial cell (EC) surface continuity is restored, the angioplasty site remains thrombogenic relative to the adjacent normal artery. Additionally, the development of intimal hyperplasia is more severe if restoration of the endothelial monolayer is delayed [2, 3]. Many factors that inhibit EC migration in vitro or delay arterial healing in vivo have been identified.…”

Section: Introductionmentioning

confidence: 99%

Apolipoprotein A-I mimetic peptide reverses impaired arterial healing after injury by reducing oxidative stress

Rosenbaum¹,

Chaudhuri²,

Abelson³

et al. 2015

Atherosclerosis

View full text Add to dashboard Cite

Objective Endothelial cell (EC) migration is essential for healing of arterial injuries caused by angioplasty, but a high cholesterol diet inhibits endothelial repair. In vivo studies suggest that apolipoprotein A-I (apoA-I), the major protein constituent of HDL, is essential for normal healing of arterial injuries. ApoA-I mimetics, including 4F, have been designed to mimic the amphipathic portion of the apoA-I molecule. This study was undertaken to determine if 4F improves endothelial migration and healing. Methods A razor scrape assay was used to analyze the effect of 4F on EC migration in vitro. Endothelial healing in vivo was assessed following electrical injury of carotid arteries in mice. Markers of oxidative stress were also examined. Results Lipid oxidation products inhibited EC migration in vitro, but preincubation with L-4F preserved EC migration. Endothelial healing of carotid arterial injuries in mice on a high cholesterol diet was delayed compared with mice on a chow diet with 27.8% vs. 48.2% healing, respectively, at 5 days. Administration of D-4F improved endothelial healing in mice on a high cholesterol diet to 43.4%. D-4F administration had no effect on lipid levels but decreased markers of oxidation. In vivo, there was a significant inverse correlation between endothelial healing and plasma markers of oxidative stress. Conclusion These studies suggested that an apoA-I mimetic can improve endothelial healing of arterial injuries by decreasing oxidative stress.

show abstract

Endothelial Recovery

Cited by 78 publications

References 45 publications

Metabolic and Structural Integrity of Magnetic Nanoparticle-Loaded Primary Endothelial Cells for Targeted Cell Therapy

Metabolic and Structural Integrity of Magnetic Nanoparticle-Loaded Primary Endothelial Cells for Targeted Cell Therapy

Porphyrin-Photosensitized Processes: Their Applications in the Prevention of Arterial Restenosis

Apolipoprotein A-I mimetic peptide reverses impaired arterial healing after injury by reducing oxidative stress

Contact Info

Product

Resources

About