Endothelialization of Magnetic Graft Materials using SPION-labeled Endothelial Cells

Newman, Brian; Dragomir-Daescu, Dan; Harbuzariu, Adriana; McIntosh, Malcolm B.; Harburn, J. Jonathan; Parakka, A.; Kalra, Manju; Holmes, David; Simari, Robert D.; Sandhu, Gurpreet S.; Häfeli, Urs O.; Schütt, Wolfgang; Zborowski, Maciej

doi:10.1063/1.3530044

Cited by 1 publication

(3 citation statements)

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“…Since they are not covalently bound to the BC, the leakage of the particles may pose toxic effects to the cells, which also makes it impossible to control the cell adhesion and growth by the magnetic field remotely. Other studies promote endothelialization by the interactions between magnetic vascular grafts or stents and the cells labeled with magnetic nanoparticles, ,, which also cannot be controlled remotely. We show that our setup regulates the adhesion and growth of endothelial cells on RGD-modified magnetic BC and applying a low (0.1 Hz) oscillating frequency could promote cell spread, adhesion, and growth and finally contribute to forming the model endothelium.…”

Section: Resultsmentioning

confidence: 99%

“…The electrical potential, 18 heat, 19 and enzymes 20 have been demonstrated to modulate the dynamic presentation of cell-adhesive ligands in vitro but mostly require direct contact with cells. Other studies use an internal magnetic field from the magnetic vascular graft or stent to attract magnetic nanoparticle-labeled endothelial cells for endothelialization 21,22 but cannot achieve non-invasive control of cell adhesion on a substrate. Magnetic materials have also shown great potential in promoting bone regeneration and cell imaging in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…The electrical potential, heat, and enzymes have been demonstrated to modulate the dynamic presentation of cell-adhesive ligands in vitro but mostly require direct contact with cells. Other studies use an internal magnetic field from the magnetic vascular graft or stent to attract magnetic nanoparticle-labeled endothelial cells for endothelialization , but cannot achieve non-invasive control of cell adhesion on a substrate. Magnetic materials have also shown great potential in promoting bone regeneration and cell imaging in vivo . − More recently, Kang et al demonstrated that by remotely tuning the frequency of ligand oscillations with a magnetic field, the adhesion of both stem cells and macrophages onto glass substrates could be regulated in vivo . , These studies drive our interests in exploring the possibility of regulating the endothelialization by controlling ligand oscillations in a non-invasive manner.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oscillating Magnetic Field Regulates Cell Adherence and Endothelialization Based on Magnetic Nanoparticle-Modified Bacterial Cellulose

Zhang

Feng

Bai

et al. 2020

ACS Appl. Mater. Interfaces

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Despite the widely explored biomaterial scaffolds in vascular tissue engineering applications lately, no ideal platform has been provided for small diameter synthetic vascular grafts mainly due to the thrombosis issue. Endothelium is the only known completely non-thrombogenic material; so, functional endothelialization onto vascular biomaterials is critical in maintaining the patency of vascular networks. Bacterial cellulose (BC) is a natural biomaterial with superior biocompatibility and appropriate hydrophilicity as potential vascular grafts. In previous studies, surface modification of active peptides such as Arg-Gly-Asp (RGD) sequences onto biomaterials has been proven to achieve accelerated and selective endothelial cell (EC) adhesion. In our study, we demonstrated a new strategy to remotely regulate the adhesion of endothelial cells based on an oscillating magnetic field and achieve successful endothelialization on the modified BC membranes. In details, we synthesized bacterial cellulose (BC), magnetic BC (MBC), and RGD peptide-grafted magnetic BC (RMBC), modified with the HOOC-PEG-COOH-coated iron oxide nanoparticles (PEG-IONs). The endothelial cells were cultured on the three materials under different frequencies of an oscillating magnetic field, including "stationary" (0 Hz), "slow" (0.1 Hz), and "fast" (2 Hz) groups. Compared to BC and MBC membranes, the cells on RMBC membranes generally show better adhesion and proliferation. Meanwhile, the "slow" frequency of a magnetic field promotes this phenomenon on RMBC and achieves endothelialization after culture for 4 days, whereas "fast" inhibits the cellular attachment. Overall, we demonstrate a non-invasive and convenient method to regulate the endothelialization process, with promising applications in vascular tissue engineering.

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Section: Resultsmentioning

confidence: 99%