Endothelium-dependent and direct relaxation induced by ethyl acetate extract from Flos Chrysanthemi in rat thoracic aorta

Jiang, Huidi; Cai, Jianfei; Xu, Juanhua; Zhou, Xinmei; Xia, Qiang

doi:10.1016/j.jep.2005.04.018

Cited by 58 publications

(35 citation statements)

References 10 publications

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“…Phenylephrine and high K + are known to increase cellular Ca ++ level by allowing Ca ++ influx through receptor-operated and voltage-dependent Ca ++ channels (VDCs), respectively (Jiang et al, 2005). We hypothesized that the inhibitory effect of the extract may involve interference with Ca ++ entry through VDCs.…”

Section: A B C Dmentioning

confidence: 99%

Mentha longifolia lowers blood pressure in anesthetized rats through multiple pathways

Shah

Bukhari

Gilani³

2016

Bangladesh J Pharmacol

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This study was aimed to investigate the effect of the extract of Mentha longifolia on blood pressure and the possible mechanisms. In anesthetized rats, the crude extract of M. longifolia and aqueous and chloroform fractions caused a dose-dependent fall in mean arterial pressure. Atropine pretreatment abolished the effect of extract and aqueous fraction but did not change that of chloroform fraction. In rabbit aortic rings, crude extract relaxed phenylephrine (1 µM) and high K+ (80 mM) pre-contractions. Chloroform fraction was more potent against high K+, similar to verapamil and caused a rightward shift in the Ca++ concentration-response curves. Aqueous fraction partially relaxed high K+ pre-contractions. In rat aortic rings, crude extract and aqueous fraction-induced endothelium-dependent atropine-sensitive vasodilator effect. Extract and fractions also relaxed high K+ precontractions. In guinea-pig atrial strips, crude extract and chloroform fraction suppressed force and rate of contractions, similar to verapamil. In conclusion, M. longifolia lowers blood pressure through Ca++ channel blockade and atropine-sensitive-NO pathway.Video Clip:<a href="https://youtube.com/v/Fz0MrZ6q2WI">Experiment using aorta:</a> 2 min 35 sec

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Section: A B C Dmentioning

confidence: 99%

Mentha longifolia lowers blood pressure in anesthetized rats through multiple pathways

Shah

Bukhari

Gilani³

2016

Bangladesh J Pharmacol

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“…While apigenin has reportedly induced vasodilation in the thoracic aorta of rats [10], its impact on the aorta of diabetic animals in isolated vascular preparations has yet to be confirmed. Therefore, the purpose of the current study was to investigate the effect of apigenin on the diabetes-associated exaggerated vasoconstriction and impaired vasodilation.…”

Section: Introductionmentioning

confidence: 98%

Normal Vascular Reactivity Is Restored by Apigenin in Diabetic Rats

El-Bassossy

Desoky

Alahdal

et al. 2018

Int J Pharm Pharm Sci

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Objective: Diabetes is a disease whose complications have serious implications for the health of sufferers; one of the most serious such complications is the deterioration of vascular reactivity. Apigenin is a natural flavonoid with PKC inhibiting and antioxidant properties. In this study, the impact of apigenin on vascular reactivity deterioration was investigated.Methods: Insulin resistance (IR) and insulin deficiency (ID) were induced by fructose and streptozotocin respectively. The isolated aortae vasoconstriction response to phenylephrine (PE) and potassium chloride (KCl) in addition to the vasodilation response to acetylcholine (ACh) and sodium nitroprusside (SNP) were tested.Results: IR and ID were associated with significantly exaggerated vasoconstriction to KCl and PE while significantly impaired vasodilation to ACh. Response to SNP was not significantly affected by both IR and ID. In vitro incubation with apigenin (7 7µM) for 20 min restored normal responses to PE, KCl and ACh in aortae isolated from insulin-resistant or insulin-deficient rats. Incubation for one hour with the PKC stimulant, phorbol 12-myristate 13-acetate (PMA, 800 nM) resulted in aortic impairment similar to that seen in aortae isolated from IR and ID animals. Incubation with both apigenin prevented PMA-induced exaggerated vasoconstriction response to both PE and KCl.Conclusion: Apigenin alleviates vascular exaggerated vasoconstriction and impaired dilation associated with diabetes or PKC activated.

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“…(Asteraceae) (CM) (Chinese Pharmacopoeia, 2010) is a medicinal and edible plant that contains a wide variety of natural compounds, including polyphenols and volatile oils (Lin & Harnly 2010;Sun et al 2010). Modern pharmacological studies have shown that CM possesses anti-inflammatory (Wu et al 2013), antioxidant (Jing et al 2015;Zheng et al 2015), antitumour (Kim et al 2013), vasodilation (Jiang et al 2005) and hepatoprotective effects (Rusu et al 2005). Our previous studies found that the polyphenol-rich CM extract (CME) could decrease hepatic lipid accumulation by increasing the PPARa expression in high fat milk-induced fatty liver mice (Cui et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Chrysanthemum morifolium extract improves hypertension-induced cardiac hypertrophy in rats by reduction of blood pressure and inhibition of myocardial hypoxia inducible factor-1alpha expression

Gao

Zhu

Zhou

et al. 2016

Pharmaceutical Biology

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(2016) Chrysanthemum morifolium extract improves hypertension-induced cardiac hypertrophy in rats by reduction of blood pressure and inhibition of myocardial hypoxia inducible factor-1alpha expression, Pharmaceutical Biology, 54:12, 2895-2900, DOI: 10.1080/13880209.2016 Context: Chrysanthemum morifolium Ramat. (Asteraceae) extract (CME) possesses a vasodilator effect in vitro. However, the use of polyphenol-rich CME in the treatment of hypertension-induced cardiac hypertrophy has not been reported. Objective: We investigated the effect of polyphenol-rich CME on hypertension-induced cardiac hypertrophy in rats and its possible mechanism of action. Materials and methods: The Sprague-Dawley rat model with cardiac hypertrophy was induced by renovascular hypertension. The blood pressure, cardiac weight index, free fatty acids (FFA) in serum and myocardium, and protein expressions of myocardial hypoxia inducible factor-1a (HIF-1a), peroxisome proliferator-activated receptor a (PPARa), carnitine palmitoyltransferase-1a (CPT-1a), pyruvate dehydrogenase kinase-4 (PDK-4) and glucose transporter-4 (GLUT-4) were measured after treating hypertensive rats with polyphenol-rich CME of anthodia 75-150 mg/kg once daily for 4 weeks. A myocardial histological examination was also conducted. Results: After CME treatment, the blood pressure, cardiac weight and cardiac weight index decreased by 5.7-9.6%, 9.2-18.4% and 10.9-20.1%, respectively, and the cardiomyocyte cross-sectional area also decreased by 8.3-30.4%. The CME treatment simultaneously decreased the FFA in serum and myocardium and protein expressions of myocardial HIF-1a and GLUT-4, and increased the protein expressions of myocardial PPARa, CPT-1a and PDK-4, especially in the CME 150 mg/kg group (p < 0.05 or p < 0.01). Discussion and conclusion: Polyphenol-rich CME may alleviate hypertensive cardiac hypertrophy in rats. Its mechanisms may be related to the reduction of blood pressure and amelioration of the myocardial energy metabolism. The latter may be attributed to the inhibition of HIF-1a expression and subsequent modulation of PPARa-mediated CPT-1a, PDK-4 and GLUT-4 expressions. ARTICLE HISTORY

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Endothelium-dependent and direct relaxation induced by ethyl acetate extract from Flos Chrysanthemi in rat thoracic aorta

Cited by 58 publications

References 10 publications

<i>Mentha longifolia</i> lowers blood pressure in anesthetized rats through multiple pathways

<i>Mentha longifolia</i> lowers blood pressure in anesthetized rats through multiple pathways

Normal Vascular Reactivity Is Restored by Apigenin in Diabetic Rats

Chrysanthemum morifolium extract improves hypertension-induced cardiac hypertrophy in rats by reduction of blood pressure and inhibition of myocardial hypoxia inducible factor-1alpha expression

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