Endothelium-dependent and -independent vasorelaxant actions and mechanisms induced by total flavonoids of Elsholtzia splendens in rat aortas

Wang, Huiping; Lü, Jianfeng; Zhang, Guolin; Li, Xuyun; Peng, Hongxia; Lü, Yuan; Zhao, Liang; Ye, Zhiguo; Bruce, Iain C.; Xia, Qiang; Qian, Ling-Bo

doi:10.1016/j.etap.2014.07.019

Cited by 22 publications

(16 citation statements)

References 34 publications

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“…26) Several flavonoids have been developed as vasodilator drugs, some with and others without an endothelium-dependent vasodilation effect. 18,27) Our previous study reported that oroxylin A, a flavone isolated from the bark of Eucommia ulmoides OLIV. could relax rat thoracic aorta and the effect was endothelium and NO dependent.…”

Section: Discussionmentioning

confidence: 99%

Vasodilatory Effect of Wogonin on the Rat Aorta and Its Mechanism Study

Zhang

Liu

et al. 2015

Biological & Pharmaceutical Bulletin

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1) These compounds might contribute to the anti-hypertensive effect collectively. However, the anti-hypertensive effect of Eucommia ulmoides OLIV. bark remains largely unclear.Pharmacological studies have revealed that Eucommia ulmoides OLIV. bark extract induce endothelium and NO-cyclic guanosine monophosphate (cGMP) dependent relaxation in the rat thoracic aorta.2) Another report demonstrated that the endothelium-dependent vascular relaxation induced by the bark extract is mediated by NO and endothelium-derived hyperpolarizing factor in small vessels.3) However, the vasorelaxing components have been unclear. Recently, we found oroxylin A and wogonin isolated from Eucommia ulmoides OLIV. bark could significantly lower the perfusion pressure.4) In the previous study, we had reported that oroxylin A could relax rat thoracic aorta and it was endothelium and NO dependent.5)The present study was undertaken to investigate vasodilatory effect of wogonin and its mechanism.Wogonin ( Fig. 1) is a flavone and has a variety of cardiovascular protective effect. It could regulate migration, proliferation and apoptosis of vascular smooth muscle cells. [6][7][8] Besides, wogonin could inhibit angiogenesis, suppress collagen deposition in cardiac fibroblasts and inhibit ischemic brain injury. [9][10][11][12] There is no evidence for vascular relaxation effect of wogonin. We describe here that wogonin, unlike oroxylin A, is an endothelium-and NO-independent vasodilatory flavonoid. One report demonstrated that wogonin offered a wide margin of safety.13) It has therapeutic potential for the treatment of cardiovascular and cerebrovascular diseases.

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Section: Discussionmentioning

confidence: 99%

Vasodilatory Effect of Wogonin on the Rat Aorta and Its Mechanism Study

Zhang

Liu

et al. 2015

Biological & Pharmaceutical Bulletin

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“…Although lowering blood pressure by relaxing arteries can greatly reduce hypertension complication such as cerebrovascular, cardiovascular, and renal disease (Wang et al. 2014 ), the common antihypertensive drugs present in the market nowadays have low effectiveness and undesired chronic side effects. Thus, seeking new effective antihypertensive drugs with minimal side effects is urgent for human populations.…”

Section: Introductionmentioning

confidence: 99%

Vasorelaxant properties of Vernonia amygdalina ethanol extract and its possible mechanism

Ch’ng

Loh

Tan

et al. 2017

Pharmaceutical Biology

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Context:Vernonia amygdalina Del. (VA) (Asteraceae) is commonly used to treat hypertension in Malaysia.Objective: This study investigates the vasorelaxant mechanism of VA ethanol extract (VAE) and analyzes its tri-step FTIR spectroscopy fingerprint.Materials and methods: Dried VA leaves were extracted with ethanol through maceration and concentrated using rotary evaporator before freeze-dried. The vasorelaxant activity and the underlying mechanisms of VAE using the cumulative concentration (0.01–2.55 mg/mL at 20-min intervals) were evaluated on aortic rings isolated from Sprague Dawley rats in the presence of antagonists.Results: The tri-step FTIR spectroscopy showed that VAE contains alkaloids, flavonoids, and saponins. VAE caused the relaxation of pre-contracted aortic rings in the presence and absence of endothelium with EC50 of 0.057 ± 0.006 and 0.430 ± 0.196 mg/mL, respectively. In the presence of Nω-nitro-l-arginine methyl ester (EC50 0.971 ± 0.459 mg/mL), methylene blue (EC50 1.203 ± 0.426 mg/mL), indomethacin (EC50 2.128 ± 1.218 mg/mL), atropine (EC50 0.470 ± 0.325 mg/mL), and propranolol (EC50 0.314 ± 0.032 mg/mL), relaxation stimulated by VAE was significantly reduced. VAE acted on potassium channels, with its vasorelaxation effects significantly reduced by tetraethylammonium, 4-aminopyridine, barium chloride, and glibenclamide (EC50 0.548 ± 0.184, 0.158 ± 0.012, 0.847 ± 0.342, and 0.304 ± 0.075 mg/mL, respectively). VAE was also found to be active in reducing Ca2+ released from the sarcoplasmic reticulum and blocking calcium channels.Conclusions: The vasorelaxation effect of VAE involves upregulation of NO/cGMP and PGI2 signalling pathways, and modulation of calcium/potassium channels, and muscarinic and β2-adrenergic receptor levels.

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“…A study on flowering E. splendens biomass indicated that the total flavonoids from the n-BuOH extracts exhibited significant concentration-dependent vasorelaxation in endothelium-intact rings of rat, which were not abolished but were significantly reduced by the removal of endothelium (Wang et al, 2014). Therefore, we focused on the n-BuOH extracts of the flowering aerial biomass of E. splendens, using macroporous resin (MR), Sephadex™ LH-20, polyamide (PA) resin, and preparative high-performance liquid chromatography (P-HPLC) columns during chromatographic column (CC) separation.…”

Section: Introductionmentioning

confidence: 99%

Apigenin-7-O-β-d-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Peng

Zhang

2016

J. Zhejiang Univ. Sci. B

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Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. Chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative highperformance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and 1 D-and 2 D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.

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Endothelium-dependent and -independent vasorelaxant actions and mechanisms induced by total flavonoids of Elsholtzia splendens in rat aortas

Cited by 22 publications

References 34 publications

Vasodilatory Effect of Wogonin on the Rat Aorta and Its Mechanism Study

Vasodilatory Effect of Wogonin on the Rat Aorta and Its Mechanism Study

Vasorelaxant properties of Vernonia amygdalina ethanol extract and its possible mechanism

Apigenin-7-O-β-d-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

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