Endothelium-dependent contraction induced by acetylcholine in the chicken ductus arteriosus involves cyclooxygenase-1 activation and TP receptor stimulation

Schuurman, Marijn J; Villamor, Eduardo

doi:10.1016/j.cbpa.2010.05.009

Cited by 12 publications

(11 citation statements)

References 54 publications

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“…The TP receptor agonist U46619-evoked contraction of chicken embryo mesenteric (present work), pulmonary (Villamor et al 2002), femoral (Zoer et al 2010a, b), CA (Lindgren et al 2010) arteries and DA (Agren et al 2007; Schuurman and Villamor 2010) (Fig. 5).…”

Section: Discussionsupporting

confidence: 52%

“…The contribution of each of these factors to endothelium-dependent relaxation varies across vascular beds and also with the physiological or pharmacological stimuli used to stimulate the endothelium (Busse et al 2002). In the chicken embryo, ACh induced an endothelium-dependent and, at least partially, NO-mediated relaxation of the DA (Agren et al 2007; Schuurman and Villamor 2010), the pulmonary (Villamor et al 2002), the femoral (Villamor et al 2002), and the carotid arteries (le Noble et al 2000) (Fig. 5).…”

Section: Discussionmentioning

confidence: 96%

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Developmental changes in mesenteric artery reactivity in embryonic and newly hatched chicks

Moonen

Villamor

2011

J Comp Physiol B

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At birth, the intestine becomes the sole site for nutrient absorption requiring a dramatic increase in blood flow. The vascular changes accompanying this transition have been partly characterized in mammals. We investigated, using wire myography, the developmental changes in chick mesenteric artery (MA) reactivity. Rings of the MA from 15-day (E15) and 19-day (E19) chicken embryos (total incubation 21 days) as well as non-fed 0–3-h-old (NH3h) and first-fed 1-day-old (NH1d) newly hatched chicks contracted in response to KCl, norepinephrine (NE), U46619, and endothelin (ET)-1 and relaxed in response to acetylcholine (ACh), sodium nitroprusside (SNP), and forskolin indicating the presence of electro- and pharmaco-mechanical coupling as well as cGMP- and cAMP-mediated relaxation. In ovo development and transition to ex ovo life was accompanied by alterations in the response of the MAs, but a different developmental trajectory was observed for each reactivity pathway tested. Thus, the contractile efficacy of KCl underwent a linear increase (E15 < E19 < NH3h < NH1d). The efficacy of NE and U46619 increased in ovo, but not ex ovo (E15 < E19 = NH3h = NH1d) and the efficacy of ET-1 peaked at E19 (E15 < E19 > NH3h = NH1d). The relaxations elicited by ACh (endothelium-dependent), SNP, and forskolin did not undergo significant developmental changes. In conclusion, the ability of chick MAs to constrict in response to pharmacological stimuli increases during the embryonic period, but no dramatic changes are induced by hatching or the first feeding. Maturation of vasodilator mechanisms precedes that of vasoconstrictor mechanisms. Alterations of the delicate balance between vasoconstrictors and vasodilators may play an important role in perinatal intestinal diseases.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 96%

Developmental changes in mesenteric artery reactivity in embryonic and newly hatched chicks

Moonen

Villamor

2011

J Comp Physiol B

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“…In the presence of U73122, an antagonist of PLC [30], or GF109203X (a PKC inhibitor), ACh-stimulated vasoconstrictions were attenuated in the present study, indicating M-receptors-PLC-PKC signaling pathway was involved [31]. Previous studies showed that ACh’s vascular effects were partly mediated by cyclooxygenase products [32]. In the present study, indomethacin inhibited the umbilical vessel contraction, indicating possible involvement of interaction between M receptors and cyclooxygenase.…”

Section: Discussionmentioning

confidence: 48%

Roles of ion channels in regulation of acetylcholine-mediated vasoconstrictions in umbilical cords of rabbit/rats

Zhu

Tang

Zhou

et al. 2016

Reproductive Toxicology

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We recently demonstrated that acetylcholine (ACh) produced reliable vasoconstrictions in the umbilical cords. This study investigated the possible mechanisms with different antagonists. ACh-mediated vasoconstrictions were decreased by voltage-operated calcium (Ca2+) channels antagonist nifedipine or inositol-1,4,5-trisphosphate-mediated Ca2+ release antagonist 2-aminoethyl diphenylborinate, indicating that both extracellular and intracellular calcium modulated the ACh-stimulated umbilical contraction. Intracellular Ca2+ concentrations were increased simultaneously with vasoconstrictions by ACh in the umbilical vessels. Inhibiting large-conductance calcium-dependent potassium (BK) channels enhanced ACh-mediated contraction, whereas inhibiting voltage dependent potassium (K+), inward rectifier K+ and ATP-sensitive K+ channels had no effects. Incubation with specific K+ channel inhibitors showed that ACh suppressed BK currents rather than 4-aminopyridine-sensitive K+ channels currents. The results suggested that blood vessels in umbilical cords had special characteristics in response to cholinergic signals. ACh-stimulated umbilical vasoconstrictions were mediated via muscarinic receptor subtype 1/3–protein kinase C/cyclooxygenase-BK channel pathways.

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“…Therefore, there is a need for additional models, addressing these limitations. In the last years, the chicken embryo has emerged as a suitable model for the study of DA vascular biology [3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: The Chicken Embryo As a Model For Ductus Arteriosus' Developmentioning

confidence: 99%

New Mediators in the Biology of the Ductus Arteriosus: Lessons from the Chicken Embryo

Sterren

Mohammed

Villamor

2020

Molecular Mechanism of Congenital Heart Disease and Pulmonary Hypertension

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The chicken embryo is an ideal model for the study of new hypotheses on the developmental biology of ductus arteriosus (DA). A unique characteristic of chicken DA is that it is the result of the fusion of two vessels with different embryological origins, morphologies, and functionalities. The pulmonary side (PulmDA) consists almost exclusively of neural crest-derived cells, shows the structure of a muscular artery, and responds to O 2 with contraction whereas the aortic part is of mesodermal origin, shows the morphology of an elastic artery and relaxes in response to O 2. In addition the two parts of the DA show marked differences in responsiveness to other contractile and relaxant agents. In mammals, the most accepted model of O 2-induced DA constriction involves a rise in O 2 modulating the function of the mitochondrial electron transport chain (the sensor), leading to an increased production of H 2 O 2 (the mediator) that causes the inhibition of K V channels (the effector) with Rho kinase acting as another downstream effector of the O 2-sensing system in the DA. In the chicken embryo, we verified the very same pathway, proving a conserved mechanism for O 2 sensing/signaling in mammalian and nonmammalian DA. Moreover, we demonstrated a developmentally regulated response to O 2 , which is restricted to the mature PulmDA and involves parallel maturation of the three components: sensor, mediator, and effectors. Besides O 2 , we used the chicken embryo model to investigate the possible ductal effects of vasoactive mediators such as ceramide, H 2 S, isoprostanes, or platelet-derived vasoactive mediators.

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Endothelium-dependent contraction induced by acetylcholine in the chicken ductus arteriosus involves cyclooxygenase-1 activation and TP receptor stimulation

Cited by 12 publications

References 54 publications

Developmental changes in mesenteric artery reactivity in embryonic and newly hatched chicks

Developmental changes in mesenteric artery reactivity in embryonic and newly hatched chicks

Roles of ion channels in regulation of acetylcholine-mediated vasoconstrictions in umbilical cords of rabbit/rats

New Mediators in the Biology of the Ductus Arteriosus: Lessons from the Chicken Embryo

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