2010
DOI: 10.1007/s12010-010-9058-1
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Endpoint Fragmentation Index: A Method for Monitoring the Evolution of Microbial Degradation of Polysaccharide Feedstocks

Abstract: We describe a simple method for tracking the course of microbial degradation of polysaccharide-rich feedstocks. The method involves determining total polysaccharides present in the feedstock, measured in glucose equivalents, relative to the fractional component of polysaccharides exhibiting 2,3-dinitrosalycylic acid aldehyde activity. The ratio of total polysaccharide to aldehyde activity, defined as the end-point fragmentation (EPF) index, is then calculated and tracked as it shifts as microbial degradation o… Show more

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