To determine the optimal pedalling rate that minimises both the oxygen consumption (fV(O2,min)) and the energy cost of cycling (f(Cr,min)), 22 male subjects were asked to cycle on an ergometer on five occasions of 4 min each at a constant power output of 150 W and at pedalling rates of 40, 60, 80, 100 and 120 rpm. The oxygen consumption (V(O2) in millilitres per minute per kilogram) and the energy cost (Cr in joules per kilogram per metre) were determined during each period. The individual V(O2)-pedalling rate and Cr-pedalling rate relationships were fitted by parabolic regressions which allowed the determination for each individual of fV(O2,min) [mean (SD) 57.0 (4.9) rpm] and f(Cr,min) [101.1 (3.2) rpm], respectively. Contrary to the values obtained for fV(O2,min), those for f(Cr,min) were in agreement with the pedalling rates (90-110 rpm) usually selected in road cycling. It is therefore suggested that the minimisation of Cr is the main factor that determines the pedalling rate in field conditions. The lack of a significant correlation between fV(O2,min) and f(Cr,min) further indicated that, although fV(O2,min) is often used for determining the metabolic capacities of subjects, f(Cr,min) is a better index of optimal mechanical parameters of cycling in field conditions.