Energy-dependent accumulation of iron by isolated rat liver mitochondria. III. Submitochondrial localization of the iron accumulated

Romslo, Inge; Flatmark, Torgeir

doi:10.1016/0005-2728(74)90041-3

Cited by 22 publications

(3 citation statements)

References 19 publications

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“…Consistent with the finding that the inner membrane is capable of iron accumulation (Romslo and Flatmark 1974), proteoliposome containing inner Figure 5. Divalent metal ions affected iron uptake.…”

Section: Discussionsupporting

confidence: 79%

Effects of ATP and ADP on iron uptake in rat heart mitochondria

Kim

Song

2010

Animal Cells and Systems

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Iron uptake in mitochondria and fractionated mitochondria compartments was studied to understand iron transport in heart mitochondria. The inner membrane is most active in iron uptake. Mitochondrial uptake was dependent on iron concentration and the amount of mitochondria. Iron transport was inversely proportional to pH in the range of 6.0 to 8.0. Iron transport reached a maximum after 30 min of incubation at 378C. Iron uptake was inhibited by 1 mM ATP and stimulated by 1 mM ADP. The oxidative phosphorylation inhibitor oligomycin inhibited iron uptake, but rotenone and antimycin A did not. The divalent ions Mg 2' , Cu 2' , Mn 2' , and Zn 2' suppressed iron uptake at 10 mM and stimulated it at 1 mM. The divalent ion Ca 2 ' stimulated iron uptake at 10 mM and suppressed it at 1 mM, competing with iron. The uptake of calcium was stimulated by 10 to 1000 mM ATP, while iron uptake was stimulated reciprocally by 10 to 1000 mM ADP, suggesting that these ions have movements similar to those of ATP and ADP.

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Section: Discussionsupporting

confidence: 79%

Effects of ATP and ADP on iron uptake in rat heart mitochondria

Kim

Song

2010

Animal Cells and Systems

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“…The outer membrane does not appear to be associated with iron to any large extent. This distribution of matrix-and inner membrane-bound iron is strongly suggestive of an energy-dependent mechanism (Romslo & Flatmark, 1974). This very rapid localization to the mitochondria supports the proposed role of mitochondrial iron as a reflection of body iron stores (Richmond et al, 1972) but in addition suggests that the mitochondria may be directly involved in the absorptive process, with iron possibly undergoing a valency change in this organelle.…”

Section: Discussionsupporting

confidence: 61%

“…Sucrose was added to a final concentration of 0.25 mol/l and the mixture kept on ice for a further 5 min (Wojtczak & Sottocasa, 1972). The suspension was then sonicated (MSE Sonicater, MSE Scientific Instruments, Crawley, Sussex, U.K.) at 20 000 cycles/s for three periods of 30 s (Romslo & Flatmark, 1974).…”

Section: Subfractionation Of Mitochondrial Fractionsmentioning

confidence: 99%

Subcellular Distribution of Radio-Labelled Iron during Intestinal Absorption in Guinea-Pig Enterocytes with Special Reference to the Mitochondrial Localization of the Iron

Hopkins

1979

Clinical Science

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1. 59Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5--30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59Fe was extremely rapid and after 5 min 45% of the radioactivity sedimented in the mitochondrial fraction. 4. Density gradient centrifugation indicated that approximately 80% of this radioactivity was associated with the mitochondria themselves; the remainder was in brush-border fragments. 5. Selective disruption of the mitochondria demonstrates that the iron is localized to the matrix and the inner membrane, indicating transport of the absorbed iron into the organelle. 6. It is suggested that mitochondria are actively implicated at an early stage in the intestinal transport of iron.

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