Energy intake following infusion of glucagon and GLP-1: a double-blind crossover study

Cegla, Jaimini; Troke, R.; Jones, Ben; Tharakan, George; McCullough, Katherine; Wilde, Julia; Lim, Chung Thong; Parvizi, Naseem; Hussein, Mohamed; Minnion, James; Cuenco, Joyceline; Chambers, Edward S.; Ghatei, Mohammad A.; Tan, Tricia; Bloom, Stephen R.

doi:10.1530/endoabs.31.oc4.5

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“…Differences employed were facilitated through literature-informed choices and their associated effects on subjective appetite, feeding behaviour and within-subject day-to-day biological variation. For plasma concentrations of GLP1 and glucagon, pre-determined time-averaged AUC differences of 1.4 pg/ml per h (2) and 4.9 pg/ml per h (22), between methodological approaches and visits were deemed clinically important discrepancies respectively. Furthermore, differences of 62.0 pmol/l per h (23) and 148.0 pg/ml per h (24) were deemed clinically important for plasma concentrations of insulin and leptin between methodological approaches and visits respectively.…”

Section: Statistical Analysesmentioning

confidence: 99%

Agreement between fingertip-capillary and antecubital-venous appetite-related peptides

Green¹,

Gonzalez²,

Thomas³

et al. 2014

Endocrine Connections

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This study examined the agreement between fingertip-capillary and antecubital-venous measures of appetite-related peptides. Simultaneous fingertip-capillary and antecubitalvenous blood samples were collected from 19 participants. The samples were obtained at baseline, 30, 60, 90, and 120 min following breakfast for the determination of plasma GLP1 7-36 , glucagon, insulin and leptin. Between-day reproducibility of fingertip-capillaryderived estimates was assessed in 18 participants. Deming regression, limits of agreement (LOA) and typical error as a coefficient of variation (CV) were used to quantify agreement (CV a ) and reproducibility (CV r ). Deming regression revealed no systematic bias for any of the analytes studied, but for insulin there was evidence of a proportional difference at higher concentrations. Measures of GLP1 7-36 (CV a Z24.0%, LOA G2.5 pg m/l per h), leptin (CV a Z9.0%, LOA !/O1.19) and glucagon (CV a Z21.0%, LOA, G31.5 pg m/l per h) revealed good agreement between methodological approaches. Fingertip-capillary glucagon was highly reproducible between days (CV r Z8.2%). GLP1 7-36 and leptin demonstrated modest reproducibility (CV r Z22.7 and 25.0% respectively). For insulin, agreement (CV a Z36.0%, LOA !/O1.79) and reproducibility were poor (CV r Z36.0%). Collectively, the data demonstrate that fingertip-capillary blood sampling provides a comparable and reproducible alternative to antecubital-venous blood sampling for the quantification of glucagon, and to a lesser extent for GLP1 7-36 and leptin. Caution should be exercised when utilising fingertip-capillary blood sampling for insulin quantification, and consequently should not be employed interchangeably with antecubital-venous blood sampling.

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Section: Statistical Analysesmentioning

confidence: 99%