Energy Management of Mature Mammalian Spermatozoa

Rodríguez‐Gil, Joan E.

doi:10.5772/51711

Cited by 4 publications

(1 citation statement)

References 51 publications

(86 reference statements)

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“…Upon ejaculation, sperm come into contact with seminal plasma (SP) and, when deposited within the female reproductive tract, they interact with uterine and oviductal fluids, which are known to differ in terms of ion 6 and metabolite [7][8][9] composition. Changes in these surrounding biofluids and, therefore, the availability of substrates and oxygen, together with the dramatic changes that sperm undergo to become fertilising competent 10 , force these cells to utilise diverse metabolic pathways, including glycolysis and oxidative phosphorylation (Oxphos) 11,12 to satisfy their energetic requirements. In addition, the metabolic pathway preferentially used by sperm is highly species-specific 12 .…”

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confidence: 99%

Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

et al. 2023

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Whether basal metabolic activity in sperm has any influence on their fertilising capacity has not been explored. Using the pig as a model, the present study investigated the relationship of energetic metabolism with sperm quality and function (assessed through computer-assisted sperm analysis and flow cytometry), and fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels of metabolites related to glycolysis, ketogenesis and Krebs cycle were determined through a targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality sperm are associated to greater levels of glycolysis-derived metabolites, and oocyte fertilisation and embryo development are conditioned by the sperm metabolic status. Interestingly, glycolysis appears to be the preferred catabolic pathway of the sperm giving rise to greater percentages of embryos at day 6. In conclusion, this study shows that the basal metabolic activity of sperm influences their function, even beyond fertilisation.

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confidence: 99%

Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

et al. 2023

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1H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant

Rahimi

Farahmand

Mirvaghefi

et al. 2019

Fish Physiol Biochem

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Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

Salas-Huetos,

Ribas-Maynou,

Mateo-Otero

et al. 2023

J Animal Sci Biotechnol

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Background MicroRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules of 22–24 nucleotides that regulate gene expression. In the last decade, miRNAs have been described in sperm of several mammals, including cattle. It is known that miRNAs can act as key gene regulators of early embryogenesis in mice and humans; however, little is known about the content, expression, and function of sperm-borne miRNAs in early bovine embryo. In this study, total sperm RNA was isolated from 29 cryopreserved sperm samples (each coming from a separate bull) using a RNeasy kit and treatment with DNase I. RNA concentration and purity were determined through an Epoch spectrophotometer and an Agilent Bioanalyzer. The expression of 10 candidate miRNAs in bovine sperm (bta-miR-10a, bta-miR-10b, bta-miR-138, bta-miR-146b, bta-miR-19b, bta-miR-26a, bta-miR-34a, bta-miR-449a, bta-miR-495 and bta-miR-7), previously identified in testis and/or epididymis, was evaluated with RT-qPCR. The cel-miR-39-3p was used as a spike-in exogenous control. Nonparametric Mann–Whitney tests were run to evaluate which miRNAs were differentially expressed between bulls with high fertility [HF; non-return rates (NRR) ranging from 39.5 to 43.5] and those with subfertility (SF; NRR ranging from 33.3 to 39.3). Several sperm functionality parameters (e.g., viability, membrane stability or oxygen consumption, among others) were measured by multiplexing flow cytometry and oxygen sensing technologies. Results RNA concentration and purity (260/280 nm ratio) (mean ± SD) from the 29 samples were 99.3 ± 84.6 ng/µL and 1.97 ± 0.72, respectively. Bioanalyzer results confirmed the lack of RNA from somatic cells. In terms of the presence or absence of miRNAs, and after applying the Livak method, 8 out of 10 miRNAs (bta-miR-10b, -138, -146b, -19b, -26a, -449a, -495, -7) were consistently detected in bovine sperm, whereas the other two (bta-miR-10a, and -34a) were absent. Interestingly, the relative expression of one miRNA (bta-miR-138) in sperm was significantly lower in the SF than in the HF group (P = 0.038). In addition to being associated to fertility potential, the presence of this miRNA was found to be negatively correlated with sperm oxygen consumption. The expression of three other miRNAs (bta-miR-19b, bta-miR-26a and bta-miR-7) was also correlated with sperm function variables. Conclusions In conclusion, although functional validation studies are required to confirm these results, this study suggests that sperm bta-miR-138 is involved in fertilization events and beyond, and supports its use as a fertility biomarker in cattle.

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Energy Management of Mature Mammalian Spermatozoa

Cited by 4 publications

References 51 publications

Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

1H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant

Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

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