Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis

Abstract

Intestinal epithelial cells continuously migrate and mature along crypt-villus axis (CVA), while the changes in energy metabolism during maturation are unclear in neonates. The present study was conducted to test the hypothesis that the energy metabolism in intestinal epithelial cells would be changed during maturation along CVA in neonates. Eight 21-day-old suckling piglets were used. Intestinal epithelial cells were isolated sequentially along CVA, and proteomics was used to analyze the changes in proteins expression in epithelial cells along CVA. The identified differentially expressed proteins were mainly involved in cellular process, metabolic process, biological regulation, pigmentation, multicellular organizational process and so on. The energy metabolism in intestinal epithelial cells of piglets was increased from the bottom of crypt to the top of villi. Moreover, the expression of proteins related to the metabolism of glucose, most of amino acids, and fatty acids was increased in intestinal epithelial cells during maturation along CVA, while the expression of proteins related to glutamine metabolism was decreased from crypt to villus tip. The expression of proteins involved in citrate cycle was also increased intestinal epithelial cells during maturation along CVA. Moreover, dietary supplementation with different energy sources had different effects on intestinal structure of weaned piglets.

The relationship between villous height and growth performance, small intestinal mucosal enzymes activities and nutrient transporters expression in weaned piglets

“…This is in agreement with previous study, which concluded that the VH was related to the nutritional state in rats (Infante, Rouanet, & Besancon, ). That could be due to the ADFI, because it had been reported that insufficient energy intake is a major factor accounting for villous atrophy (Wang et al, ; Xiong, Yang, Tan, et al, ; Yang, Wang, Xiong, & Yin, ; Yang, Xiong, Wang, Li, & Yin, ). Marion et al () also reported that shortened small intestinal villus associates with absorptive capacity reductions.…”

The production of short chain fatty acid and colonic development in weaning piglets

“…Colonic growth parameters, including weight and length, were greater on day 7 and 14 post‐weaning, which is similar to previous studies (Marion, Biernat, Thomas, Le, & Zabielski, ; Tsukahara et al, ). Sufficiently rapid gut epithelium renewal, such as proliferation and differentiation, is a pre‐requisite for colon normal growth (Kohlnhofer, Thompson, Walker, & Battle, ; Yang, Wang, Xiong, & Yin, ). Crypt fission, which creates new crypts, is an alternate means of increasing intestinal tissue mass (Park et al, ).…”

Energy Homeostasis of the Artificial Bladder in Experimental Conditions

“…The results of the studies performed by different authors indicate high respiratory activity of the intestinal mucosa of experimental animals, which corresponds to the intestine as a highly aerobic organ with a high intensity of energy exchange [17,18].…”