Energy migration in allophycocyanin‐B trimer with a linker polypeptide: Analysis by the principal multi‐component spectral estimation (PMSE) method

Abstract

Energy migration processes in allophycocyanin-B trimer with a linker polypeptide were analyzed using the principal multi-component spectral estimation (PMSE) method, which does not require assumption of component number, decay function, or the spectral band shape. We determined the number of spectral components showing independent kinetic behavior by the eigenvalue of an auto-correlation matrix, and further the spectra of the components and their rise and decay curves. Two decay components were resolved at 20% one corresponded to the decay of one type of /%4 chromophore, and the other to the decay from the thermally equilibrated state between another type of p-84 chromophore and the cc-allophycocyanin B chromophore. An additional slow decay process was resolved at -196°C. We also compared the component spectra obtained using the PMSE method with the decay-associated spectra obtained using the global analysis.

Variations in Photosystem I Properties in the Primordial Cyanobacterium Gloeobacter violaceus PCC 7421

“… Global analysis on time‐resolved fluorescence spectra of the PS I complex at 77 K. The number of components was estimated by SVD analysis and the component spectra were obtained by global analysis (22). Left: Spectra of Gloeobacter violaceus and right: of Synechocystis sp.…”

“…The low‐temperature fluorescence spectra were resolved to components by global analysis. Prior to global analysis, single value decomposition (SVD) analysis (22) was applied to the whole set of decay datasets to estimate an appropriate number of components. On the basis of the SVD results, global analysis was then applied (23).…”