system as fertilizer for plants. In this paper, we analyse the operational performance of three aquaponic systems. Two systems were located in Iceland, and one in northern Spain. We also analyse the energy output with respect to edible protein contents. After 10 years of partially simulated operation, the EROI of the Hondarribia, Sudarvogur and Akur systems was 0.055:1, 0.016:1 and 0.106:1, respectively. Our results indicate that aquaponic operations benefit from operating within a greenhouse and that direct electricity consumption is the largest energy input in the aquaponics systems. The aquaponics systems studied returned one half to one tenth the EROI as compared to conventional fisheries or aquaculture.