Energy transfer in the B800-850-carotenoid light-harvesting complex of various mutants of Rhodopseudomonas sphaeroides and of Rhodopseudomonas capsulata

Grondelle, Rienk van; Kramer, Herman J. M.; Rijgersberg, C.P.

doi:10.1016/0005-2728(82)90100-1

Cited by 92 publications

(50 citation statements)

References 17 publications

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“…Already early quantum yield measurements of the B800 fluorescence showed picosecond energy transfer from B800 to B850 at low temperature [49]. Later picosecond absorption [47,48, 501 and spectral hole-burning experiments [18-21, 541 gave a 2.2-2.5 ps energy transfer at liquid helium temperature, suggesting a B800 -B850 distance of about 1.7 nm [54], close to the value obtained from the structure.…”

Section: Excitation Transfersupporting

confidence: 54%

Photosynthetic antennae. Photosynthetic light‐harvesting

Grondelle

Monshouwer

Valkūnas

1996

Ber Bunsenges Phys Chem

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The peripheral light-harvesting complex of the photosynthetic bacterium Rhodopseudomonas acidophila (LH2) and the major plant light-harvesting complex LHCII have a very similar function: to absorb solar photons and to transfer the electronic excitation to the pigments surrounding the reaction center, the so-called 'core'. Nevertheless, their structures exhibit a dramatically different arrangement of the pigments. In LH2 the bacteriochlorophyll molecules are arranged in a highly symmetric ring, while in LHCII the positioning of the chlorophylls is very irregular. In both complexes the average distance between the pigments is 1 nm or less and, as a consequence, the electronic interaction between the pigments is strong (> 100 cm-I). Therefore, the excitation transport in these photosynthetic light-harvesting systems can not be described by a simple Fdrster type transfer mechanism, but new or other transfer mechanisms may be operative, for instance a mechanism in which the excitation is to some extent delocalized. Crucial parameters are the strength of the electronic coupling, the amount of energetic disorder and/or heterogeneity and the nature and strength of the interactions of the pigments with the protein. Here we will discuss the current status of the field of photosynthetic energy transfer in particular for LH2. We will evaluate a few simple models that contain some of the essential ingredients to describe the process of energy transfer and finally we will discuss some of the perspectives in this scientific field.

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Section: Excitation Transfersupporting

confidence: 54%

Photosynthetic antennae. Photosynthetic light‐harvesting

Grondelle

Monshouwer

Valkūnas

1996

Ber Bunsenges Phys Chem

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“…The obtained reorganization energy λ (Table 1) corresponding to the half of the Stokes shift has been found to be somewhat smaller for the B800 Bchl molecules in PVA than for the glycerol / buffer solution; that compares well with the experimental data [29]. The reorganization energy for B850 is approximately two times larger than that for B800 and is similar to the value found by Rutkauskas et al [30] and Freiberg et al [31] when simulating the fluorescence spectra.…”

Section: Discussionsupporting

confidence: 85%

Estimation of the spectral density function of LH2 complexes from the temperature dependence of the absorption spectra

Vrublevskaja¹

2006

Lithuanian J. Phys.

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The spectral density function of a bacteriochlorophyll molecule in its parametric form describing the exciton coupling to the vibrational bath including the protein and the solvent modes has been determined. This form of spectral density is applied by simulating the temperature dependence of the absorption spectra of the peripheral light harvesting complexes, LH2, of the photosynthetic purple bacterium Rhodobacter sphaeroides inserted into the polyvinyl alcohol film. It has been found that the dynamic characteristics of broadening of both absorption bands of LH2 with the maxima at 850 nm and 800 nm are similar to those already obtained for the glycerol solution. The static spectral inhomogeneity of LH2 in the polyvinyl alcohol film, however, is much larger than in the glycerol solution. It is concluded that the LH2 complex in the polyvinyl alcohol film with increase of the temperature is subjected to compression, which is the source of the additional static disorder.

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“…The steady-state fluorescence spectrum of the isolated B875(-896) complex at 77 K [19] gives f(A), and kF = 5.6 x 107 s -I is obtained from the literature [20]. Since the solvent is mostly glycerol we have used n = 1.5 as the refractive in-dex [21].…”

Section: Resultsmentioning

confidence: 99%

Characterization of excitation energy trapping in photosynthetic purple bacteria at 77 K

1989

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We have studied the energy-transfer dynamics in chromatophores of Rhodobacter sphaeroides and Rhodospirillum rubrum at 77 K, with functional charge separation. Using low-intensity picosecond absorption recovery, we determined that transfer between the energetically low-lying antenna component BCh1896 and the special pair of the reaction center occurs with a time constant of 37 ps in Rb. sphaeroides and 75 ps in R. rubrum. Assuming that a F6rster energy-transfer mechanism applies to the process, this allows us to estimate the distance between BCh1896 in the B875 complex and the special pair P870 in the reaction center to range between 26 and 39/~, in Rb. sphaeroides. Such a distance indicates that the BChI896 pigment and the special pair of the reaction center are at the minimum separation allowed by the size and shape of the reaction center and the light-harvesting polypeptides.

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Energy transfer in the B800-850-carotenoid light-harvesting complex of various mutants of Rhodopseudomonas sphaeroides and of Rhodopseudomonas capsulata

Cited by 92 publications

References 17 publications

Photosynthetic antennae. Photosynthetic light‐harvesting

Photosynthetic antennae. Photosynthetic light‐harvesting

Estimation of the spectral density function of LH2 complexes from the temperature dependence of the absorption spectra

Characterization of excitation energy trapping in photosynthetic purple bacteria at 77 K

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