Engineered Tissue for Cardiac Regeneration: Current Status and Future Perspectives

Li, Jun Jun; Liu, Li; Zhang, Jingbo; Qu, Xiaodong; Kawamura, Takuji; Miyagawa, Shigeru; Sawa, Yoshiki

doi:10.3390/bioengineering9110605

Cited by 6 publications

(6 citation statements)

References 128 publications

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“…To this purpose, hPSC‐CMs were used as a valuable and widely recognized cell model for cardiac studies. [ 27 ] As the photoactive material counterpart, we employ a low bandgap conjugated polymer, namely PCPDTBT, characterized by far‐red optical absorption, excellent charge photogeneration efficiency, and optimal photoelectrochemical stability in a biological environment.…”

Section: Introductionmentioning

confidence: 99%

Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range

Ronchi,

Galli,

Tullii

et al. 2023

Advanced Science

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Optical stimulation in the red/near infrared range recently gained increasing interest, as a not‐invasive tool to control cardiac cell activity and repair in disease conditions. Translation of this approach to therapy is hampered by scarce efficacy and selectivity. The use of smart biocompatible materials, capable to act as local, NIR‐sensitive interfaces with cardiac cells, may represent a valuable solution, capable to overcome these limitations. In this work, a far red‐responsive conjugated polymer, namely poly[2,1,3‐benzothiadiazole‐4,7‐diyl[4,4‐bis(2‐ethylhexyl)−4H‐cyclopenta[2,1‐b:3,4‐b’]dithiophene‐2,6‐diyl]] (PCPDTBT) is proposed for the realization of photoactive interfaces with cardiomyocytes derived from pluripotent stem cells (hPSC‐CMs). Optical excitation of the polymer turns into effective ionic and electrical modulation of hPSC‐CMs, in particular by fastening Ca2+ dynamics, inducing action potential shortening, accelerating the spontaneous beating frequency. The involvement in the phototransduction pathway of Sarco‐Endoplasmic Reticulum Calcium ATPase (SERCA) and Na+/Ca2+ exchanger (NCX) is proven by pharmacological assays and is correlated with physical/chemical processes occurring at the polymer surface upon photoexcitation. Very interestingly, an antiarrhythmogenic effect, unequivocally triggered by polymer photoexcitation, is also observed. Overall, red‐light excitation of conjugated polymers may represent an unprecedented opportunity for fine control of hPSC‐CMs functionality and can be considered as a perspective, noninvasive approach to treat arrhythmias.

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Section: Introductionmentioning

confidence: 99%

Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range

Ronchi,

Galli,

Tullii

et al. 2023

Advanced Science

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“…Therefore, there is a need to further improve engraftment. It has been suggested that engraftment could be improved through tissue engineering and alternative methods of transplantation [ 118 ].…”

Section: Challenges For Hpsc-based Regenerative Therapies In Hfmentioning

confidence: 99%

“…An alternative approach for the transplant of iPSCs is to use tissue engineering to produce sheets of cells, or ‘patches’, with specific architecture mimicking the structure that biological tissues achieve via encapsulation of cells in an extracellular matrix. These patches can be attached to the epicardial surface of the heart with adhesives or sutures [ 118 , 120 ].…”

Section: Challenges For Hpsc-based Regenerative Therapies In Hfmentioning

confidence: 99%

“…Patches also have potential disadvantages in that their application may be more invasive than catheter-based delivery. Moreover, the epicardium and pericardial adipose tissue on the ventricular free wall [ 118 , 124 ] may present barriers that interfere with the full integration of the cells into the host myocardium. Epicardial patches may also be separated from the host myocardium by scar tissue, which may hinder electrical coupling with host CMs.…”

Section: Challenges For Hpsc-based Regenerative Therapies In Hfmentioning

confidence: 99%

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Unlocking the Pragmatic Potential of Regenerative Therapies in Heart Failure with Next-Generation Treatments

Kishino

Fukuda

2023

Biomedicines

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Patients with chronic heart failure (HF) have a poor prognosis due to irreversible impairment of left ventricular function, with 5-year survival rates <60%. Despite advances in conventional medicines for HF, prognosis remains poor, and there is a need to improve treatment further. Cell-based therapies to restore the myocardium offer a pragmatic approach that provides hope for the treatment of HF. Although first-generation cell-based therapies using multipotent cells (bone marrow-derived mononuclear cells, mesenchymal stem cells, adipose-derived regenerative cells, and c-kit-positive cardiac cells) demonstrated safety in preclinical models of HF, poor engraftment rates, and a limited ability to form mature cardiomyocytes (CMs) and to couple electrically with existing CMs, meant that improvements in cardiac function in double-blind clinical trials were limited and largely attributable to paracrine effects. The next generation of stem cell therapies uses CMs derived from human embryonic stem cells or, increasingly, from human-induced pluripotent stem cells (hiPSCs). These cell therapies have shown the ability to engraft more successfully and improve electromechanical function of the heart in preclinical studies, including in non-human primates. Advances in cell culture and delivery techniques promise to further improve the engraftment and integration of hiPSC-derived CMs (hiPSC-CMs), while the use of metabolic selection to eliminate undifferentiated cells will help minimize the risk of teratomas. Clinical trials of allogeneic hiPSC-CMs in HF are now ongoing, providing hope for vast numbers of patients with few other options available.

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“…Several studies have demonstrated the potential of induced pluripotent stem cell-derived CMs (iPSC-CMs) to engraft and improve the performance of myocardium after MI [ [16] , [17] , [18] , [19] , [20] , [21] ]. Several iPSC-CMs are currently in the clinical trial phase [ 22 ]. However, iPSC-CMs exhibit limited retention and poor paracrine function after transplantation.…”

Section: Introductionmentioning

confidence: 99%

Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

Zhang,

Li,

et al. 2024

Bioactive Materials

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Engineered Tissue for Cardiac Regeneration: Current Status and Future Perspectives

Cited by 6 publications

References 128 publications

Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range

Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range

Unlocking the Pragmatic Potential of Regenerative Therapies in Heart Failure with Next-Generation Treatments

Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

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