Engineering a 3D vascular network in hydrogel for mimicking a nephron

Mu, Xuan; Zheng, Wenfu; Xiao, Le; Zhang, Wei; Jiang, Xingyu

doi:10.1039/c3lc41342j

Cited by 102 publications

(70 citation statements)

References 44 publications

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“…A collagen microfluidic structure was used to pattern a tube of Madin-Darby canine kidney (MDCK) cells, next to a tube of human umbilical vein endothelial cells (HUVEC) [45]. Huang et al also show a perfusable, stratified MDCK co-culture with adipose derived stem cells [46], which enhanced cilia formation and increased expression of ion transporters of the MDCK cells.…”

Section: Vasculaturementioning

confidence: 98%

Microfluidic 3D cell culture: from tools to tissue models

Duinen

Trietsch

Joore

et al. 2015

Current Opinion in Biotechnology

442

328

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The transition from 2D to 3D cell culture techniques is an important step in a trend towards better biomimetic tissue models. Microfluidics allows spatial control over fluids in micrometer-sized channels has become a valuable tool to further increase the physiological relevance of 3D cell culture by enabling spatially controlled co-cultures, perfusion flow and spatial control over of signaling gradients. This paper reviews most important developments in microfluidic 3D culture since 2012. Most efforts were exerted in the field of vasculature, both as a tissue on its own and as part of cancer models. We observe that the focus is shifting from tool building to implementation of specific tissue models. The next big challenge for the field is the full validation of these models and subsequently the implementation of these models in drug development pipelines of the pharmaceutical industry and ultimately in personalized medicine applications.

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Section: Vasculaturementioning

confidence: 98%

Microfluidic 3D cell culture: from tools to tissue models

Duinen

Trietsch

Joore

et al. 2015

Current Opinion in Biotechnology

442

328

View full text Add to dashboard Cite

show abstract

“…In these organ-on-chips models, some key aspects of organ-level microenvironment are mimicked and applied to cells or organ-like tissues by engineering and integrating the complex components. Various "organ-on-a-chip" systems, such as blood vessel [112][113][114][115][116][117][118][119][120][121][122], lung [123][124][125][126][127][128][129], heart [130][131][132][133][134][135][136][137], kidney [138][139][140][141][142], liver [143][144][145][146][147][148], brain [149,150], and gut [151], have been reported to reproduce target organ structures and functions better than conventional in vitro model systems. In the following section, we will introduce latest progress in "organ-on-a-chip" in the context of precise manipulation of cells on interfaces.…”

Section: Organs On Chipsmentioning

confidence: 99%

“…Recapitulation of functional microvascular structures in vitro could provide a platform for the study of complex vascular phenomena, including angiogenesis and thrombosis. We [142] reported a microfluidic method that utilized fibrillogenesis of collagen and liquid mold to engineer 3D vascular networks in hydrogel. This technique enables the mimicry of passive diffusion in nephron and would be used for in vitro modeling of other vasculature-rich tissues and organs.…”

Section: Vasculature and Angiogenesismentioning

confidence: 99%

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Zheng

Jiang

2014

Colloids and Surfaces B: Biointerfaces

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“…Another platform suited for DNA delivery is based on hydrogel because of its high loading capacity, mild working conditions, and good biocompatibility [62]. Supramolecular hydrogel is a kind of hydrogel composed of nanofibers formed by the self-assembly of small molecules (molecular weight usually <2000) in aqueous solutions [63].…”

Section: Immunotherapy and Vaccinementioning

confidence: 99%

History and Applications of Hydrogels

Yahia¹

2015

J Biomed Sci

162

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Hydrogels have existed for more than half century, providing one of the earliest records of crosslinked hydroxyethyl methacrylate (HEMA) hydrogels. Today, hydrogels still fascinate material scientists and biomedical researchers and great strides have been made in terms of their formulations and applications. As a class of material, hydrogels are unique, they consist of a self-supporting, waterswollen three-dimensional (3D) viscoelastic network which permits the diffusion and attachment of molecules and cells. However, hydrogels have recently drawn great attention for use in a wide variety of biomedical applications such as cell therapeutics, wound healing, cartilage/bone regeneration and the sustained release of drugs. This is due to their biocompatibility and the similarity of their physical properties to natural tissue. This review aims to give an overview of the historic and the recent design concept of hydrogels and their several applications based on the old and the most recent publications in this field. Journal of Biomedical Sciences ISSN 2254-609XThis Article is Available in: www.jbiomeds.com 2 Hydrogels can be classified into two distinct categories, the natural and the synthetic hydrogels. Natural hydrogels include collagen, fibrin, hyaluronic acid, matrigel, and derivatives of natural materials such as chitosan, alginate and skill fibers. They remain the most physiological hydrogels as they are components of the extracellular matrix (ECM) in vivo. Two main drawbacks of natural hydrogels, however, make their final microstructures and properties difficult to control reproducibly between experiments. First, the fine details of their mechanical properties and their dependence on polymerization or gelation conditions are often poorly understood. Second, due to their natural origin (bovine fibrinogen, rat tail collagen… their composition may vary from one batch to another.In contrast, synthetic hydrogels such as poly (ethylene glycol) diacrylate, poly(acryl amide), poly(vinyl alcohol) are more reproducible, although their final structure can also depend on polymerization conditions in a subtle way, so that a rigorous control of the preparation protocol, including temperature and environment control, may be necessary. Generally speaking, synthetic hydrogels offer more flexibility for tuning chemical composition and mechanical properties; users can, for example vary the concentration or molecular weight of the precursor, or alter the percentage of crosslinkers. They can also be selected or tuned to be hydrolysable or biodegradable over variable periods of time.Hydrogels may be chemically stable or they may degrade and eventually disintegrate and dissolve. They are called «reversible» or «physical» gels when the networks are held together by molecular entanglements, and/or secondary forces including ionic, H-bonding or hydrophobic forces (Figure 2) [3]. Physical hydrogels are not homogeneous, since clusters of molecular entanglements, or hydrophobically-or ionically-associated domains, can create in h...

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Engineering a 3D vascular network in hydrogel for mimicking a nephron

Cited by 102 publications

References 44 publications

Microfluidic 3D cell culture: from tools to tissue models

Microfluidic 3D cell culture: from tools to tissue models

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

History and Applications of Hydrogels

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