Engineering a macroporous oxygen-generating scaffold for enhancing islet cell transplantation within an extrahepatic site

Liang, Jia-Pu; Accolla, Robert P.; Soundirarajan, Madhuvanthi; Emerson, Amy E.; Coronel, María M.; Stabler, Cherie L.

doi:10.1016/j.actbio.2021.05.028

Cited by 45 publications

(28 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several prior studies have focused on selecting the ideal material for encapsulating pancreatic islets. Scientists have concentrated on tailoring macroporous hybrid scaffolds of natural and synthetic polymers, which have the oxygen-generating or vascularization-enhancing ability [ 89 ] . OxySit is an in situ oxygen-generating hydrolytically active biomaterial in the form of solid calcium peroxide encapsulated in polydimethylsiloxane (PDMS) [ 90 ] .…”

Section: Scaffold-based and Scaffold-free Strategiesmentioning

confidence: 99%

Three-dimensional bioprinting of functional β-islet-like constructs

Parvaneh¹,

Kemény²,

Ghaffarinia³

et al. 2023

IJB

View full text Add to dashboard Cite

Diabetes is an autoimmune disease that ensues when the pancreas does not deliver adequate insulin or when the body cannot react to the existing insulin. Type 1 diabetes is an autoimmune disease defined by continuous high blood sugar levels and insulin deficiency due to β-cell destruction in the islets of Langerhans (pancreatic islets). Long-term complications, such as vascular degeneration, blindness, and renal failure, result from periodic glucose-level fluctuations following exogenous insulin therapy. Nevertheless, the shortage of organ donors and the lifelong dependency on immunosuppressive drugs limit the transplantation of the entire pancreas or pancreas islet, which is the therapy for this disease. Although encapsulating pancreatic islets using multiple hydrogels creates a semi-privileged environment to prevent immune rejection, hypoxia that occurs in the core of the capsules is the main hindrance that should be solved. Bioprinting technology is an innovative process in advanced tissue engineering that allows the arranging of a wide array of cell types, biomaterials, and bioactive factors as a bioink to simulate the native tissue environment for fabricating clinically applicable bioartificial pancreatic islet tissue. Multipotent stem cells have the potential to be a possible solution for donor scarcity and can be a reliable source for generating autograft and allograft functional β-cells or even pancreatic islet-like tissue. The use of supporting cells, such as endothelial cells, regulatory T cells, and mesenchymal stem cells, in the bioprinting of pancreatic islet-like construct could enhance vasculogenesis and regulate immune activity. Moreover, scaffolds bioprinted using biomaterials that can release oxygen postprinting or enhance angiogenesis could increase the function of β-cells and the survival of pancreatic islets, which could represent a promising avenue.

show abstract

Section: Scaffold-based and Scaffold-free Strategiesmentioning

confidence: 99%

Three-dimensional bioprinting of functional β-islet-like constructs

Parvaneh¹,

Kemény²,

Ghaffarinia³

et al. 2023

IJB

View full text Add to dashboard Cite

show abstract

“…Although hybrid constructs with OxySite ® showed improved viability and function in vivo [ 74 ], the manufacturing process was mostly manual and has yet not been scaled up to human application. Incorporation of OxySite ® microbeads [ 75 ] into bioinks and application in bioprinting technology might be a focus for future studies.…”

Section: Strategies Before 3d Bioprinting: Materials Properties and B...mentioning

confidence: 99%

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Salg

Blaeser

Gerhardus

et al. 2022

IJMS

View full text Add to dashboard Cite

Among advanced therapy medicinal products, tissue-engineered products have the potential to address the current critical shortage of donor organs and provide future alternative options in organ replacement therapy. The clinically available tissue-engineered products comprise bradytrophic tissue such as skin, cornea, and cartilage. A sufficient macro- and microvascular network to support the viability and function of effector cells has been identified as one of the main challenges in developing bioartificial parenchymal tissue. Three-dimensional bioprinting is an emerging technology that might overcome this challenge by precise spatial bioink deposition for the generation of a predefined architecture. Bioinks are printing substrates that may contain cells, matrix compounds, and signaling molecules within support materials such as hydrogels. Bioinks can provide cues to promote vascularization, including proangiogenic signaling molecules and cocultured cells. Both of these strategies are reported to enhance vascularization. We review pre-, intra-, and postprinting strategies such as bioink composition, bioprinting platforms, and material deposition strategies for building vascularized tissue. In addition, bioconvergence approaches such as computer simulation and artificial intelligence can support current experimental designs. Imaging-derived vascular trees can serve as blueprints. While acknowledging that a lack of structured evidence inhibits further meta-analysis, this review discusses an end-to-end process for the fabrication of vascularized, parenchymal tissue.

show abstract

“…However, the clinical application of islet transplantation is still limited due to the shortage of organ donors and other challenges. In recent years, human and rodent studies have reported the successful generation of functional islets or islet organoids with insulin secretory capacity in response to glucose stimulation from embryonic stem cells, pluripotent stem cells, or dissociated islet cells. − Meanwhile, approaches that could better accommodate the pancreatic islets during transplantation, such as implementing bioscaffold or islet encapsulation, have been reported. , However, more efforts are required to validate protocol reproducibility and improve islet cell viability and stability as well as post-transplantation efficacy.…”

Section: Introductionmentioning

confidence: 99%

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

et al. 2023

View full text Add to dashboard Cite

Pancreatic islet transplantation is a promising treatment that could potentially reverse diabetes, but its clinical applicability is severely limited by a shortage of organ donors. Various cell loading approaches using polymeric porous microspheres (PMs) have been developed for tissue regeneration; however, PM-based multicellular artificial pancreatic islets' construction has been scarcely reported. In this study, MIN6 (a mouse insulinoma cell line) and MS1 (a mouse pancreatic islet endothelial cell line) cells were seeded into poly(lacticco-glycolic acid) (PLGA) PMs via an upgraded centrifugation-based cell perfusion seeding technique invented and patented by our group. Cell morphology, distribution, viability, migration, and proliferation were all evaluated. Results from glucose-stimulated insulin secretion (GSIS) assay and RNA-seq analysis suggested that MIN6 and MS1-loaded PLGA PMs exhibited better glucose responsiveness, which is partly attributable to vascular formation during PM-dependent islet construction. The present study suggests that the PLGA PM-based artificial pancreatic islets may provide an alternative strategy for the potential treatment of diabetes in the future.

show abstract

Engineering a macroporous oxygen-generating scaffold for enhancing islet cell transplantation within an extrahepatic site

Cited by 45 publications

References 80 publications

Three-dimensional bioprinting of functional β-islet-like constructs

Three-dimensional bioprinting of functional β-islet-like constructs

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

Contact Info

Product

Resources

About