Engineering a stable future for DNA-origami as a biomaterial

Bila, Hale; Kurisinkal, Eva E.; Bastings, Maartje M. C.

doi:10.1039/c8bm01249k

Cited by 134 publications

(112 citation statements)

References 68 publications

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“…DNA origami is emerging as a precise and functional nanomaterial for various biomedical and chemical applications. However, it would be highly desirable to circumvent the intrinsic stability issues and push the limits towards a robust but still versatile nanomaterial in various environments . Apart from nuclease‐mediated digestion, magnesium ions are crucial for DNA origami assembly, but their relatively high concentration (10–20 m m ) can interfere with certain applications .…”

Section: Figurementioning

confidence: 99%

“…However, it would be highly desirable to circumvent the intrinsic stability issues and push the limits towards a robust but still versatile nanomaterial in various environments. [23] Apart from nuclease-mediated digestion, magnesium ions are crucial for DNA origami assembly, but their relatively high concentration (10-20 mm) can interfere with certain applications. [24] Hence, polydopamine coating was anticipated as an suitable tool to contribute to stability.…”

Section: Angewandte Chemiementioning

confidence: 99%

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Photocontrolled Dopamine Polymerization on DNA Origami with Nanometer Resolution

Winterwerber

Harvey

et al. 2019

Angew Chem Int Ed

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Temporal and spatial control over polydopamine formation on the nanoscale can be achieved by installing an irradiation‐sensitive polymerization system on DNA origami. Precisely distributed G‐quadruplex structures on the DNA template serve as anchors for embedding the photosensitizer protoporphyrin IX, which—upon irradiation with visible light—induces the multistep oxidation of dopamine to polydopamine, producing polymeric structures on designated areas within the origami framework. The photochemical polymerization process allows exclusive control over polydopamine layer formation through the simple on/off switching of the light source. The obtained polymer–DNA hybrid material shows significantly enhanced stability, paving the way for biomedical and chemical applications that are typically not possible owing to the sensitivity of DNA.

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Section: Figurementioning

confidence: 99%

Section: Angewandte Chemiementioning

confidence: 99%

Photocontrolled Dopamine Polymerization on DNA Origami with Nanometer Resolution

Winterwerber

Harvey

et al. 2019

Angew Chem Int Ed

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“…[119] They were shown to spontaneouslyi nsert into lipid bilayers, where they display stochastic gating behaviour and ac onductance of 70-100 pS. [125][126][127][128][129][130][131][132][133] DNA origami uses al ong, single "scaffold" strand that is folded into the desired shapeb yb inding to many short "staple" strands. [122,123] .…”

Section: Protein Engineeringmentioning

confidence: 99%

Artificial Signal Transduction across Membranes

2019

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A key conundrum in the construction of an artificial cell is to simultaneously maintain a robust physical barrier to the external environment, while also providing efficient exchange of information across this barrier. Biomimicry provides a number of avenues by which such requirements might be met. Herein, we provide a brief introduction to the challenges facing this field and explore progress to date.

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“…In the case of drug delivery, there are a variety of nanocages that can host different drug molecules. While these have been studied individually in different projects, there is lack of general design rules or parameters that can help to create drug delivery vehicles with greater potential . We propose that a generalized design strategy be developed among the DNA nanotechnology community, and a database of currently designed and characterized DNA nanostructures will help to streamline production and applicability in many settings.…”

Section: Conclusion and Outlooksmentioning

confidence: 99%

A Molecular Hero Suit for In Vitro and In Vivo DNA Nanostructures

Kizer

Linhardt

Chandrasekaran

et al. 2019

Small

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Precise control of DNA base pairing has rapidly developed into a field full of diverse nanoscale structures and devices that are capable of automation, performing molecular analyses, mimicking enzymatic cascades, biosensing, and delivering drugs. This DNA‐based platform has shown the potential of offering novel therapeutics and biomolecular analysis but will ultimately require clever modification to enrich or achieve the needed “properties” and make it whole. These modifications total what are categorized as the molecular hero suit of DNA nanotechnology. Like a hero, DNA nanostructures have the ability to put on a suit equipped with honing mechanisms, molecular flares, encapsulated cargoes, a protective body armor, and an evasive stealth mode.

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Engineering a stable future for DNA-origami as a biomaterial

Abstract: Reviewing the various methods and effectivity to stabilize DNA origami in biological environments.

Cited by 134 publications

References 68 publications

Photocontrolled Dopamine Polymerization on DNA Origami with Nanometer Resolution

Photocontrolled Dopamine Polymerization on DNA Origami with Nanometer Resolution

Artificial Signal Transduction across Membranes

A Molecular Hero Suit for In Vitro and In Vivo DNA Nanostructures

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