Engineering cell alignment in vitro

Li, Yuhui; Huang, Guangsu; Zhang, Xiaohui; Wang, Lin; Du, Yanan; Lu, Tian Jian; Xu, Feng

doi:10.1016/j.biotechadv.2013.11.007

Cited by 235 publications

(263 citation statements)

References 217 publications

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“…The degree to which strain, relative to other factors known to induce muscle myofiber differentiation such as engineering cell alignment through topographical patterns (e.g., grooves, pillars, and wrinkles) on 2D substrata, affected our observations is an important point to consider. 3 We note that although μMACs present cells with a uniform hydrogel ECM, straining provides both mechanical and steric directional cues to encapsulated cells, as the straining of the hydrogel induces anisotropy in the hydrogel microstructure that the cells may be able to sense. To ensure the suitability of μMACs for long-term culture, degradation of the GelMA hydrogels was assessed because proteolytic enzymes (e.g., gelatinase and collagenases) secreted by encapsulated cells can degrade GelMA.…”

Section: Resultsmentioning

confidence: 89%

“…[1][2][3][4] Cells sense and respond to mechanical cues, such as the mechanical strains and the rigidity of their extracellular matrix (ECM), by adjusting the transmembrane molecules (e.g., integrins) and by reorganizing the intracellular cytoskeleton. 5,6 Tools for studying strain responses of cells in two-dimensional (2D) environment, including stretchable substrata, 7 micropost arrays 8 and 2D traction microscopy, 9 are well established.…”

Section: Introductionmentioning

confidence: 99%

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Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Huang

Gao

et al. 2016

NPG Asia Mater

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Living cells respond to their mechanical microenvironments during development, healing, tissue remodeling and homeostasis attainment. However, this mechanosensitivity has not yet been established definitively for cells in three-dimensional (3D) culture environments, in part because of challenges associated with providing uniform and consistent 3D environments that can deliver a large range of physiological and pathophysiological strains to cells. Here, we report microscale magnetically actuated, cell-laden hydrogels (μMACs) for investigating the strain-induced cell response in 3D cultures. μMACs provide high-throughput arrays of defined 3D cellular microenvironments that undergo reversible, relatively homogeneous deformation following non-contact actuation under external magnetic fields. We present a technique that not only enables the application of these high strains (60%) to cells but also enables simplified microscopy of these specimens under tension. We apply the technique to reveal cellular strain-threshold and saturation behaviors that are substantially different from their 2D analogs, including spreading, proliferation, and differentiation. μMACs offer insights for mechanotransduction and may also provide a view of how cells respond to the extracellular matrix in a 3D manner.

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Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Huang

Gao

et al. 2016

NPG Asia Mater

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“…Since many tissues, e.g., striated muscle, 6 cartilage, 7 or cornea 8 , to name just a few examples, have anisotropic hierarchical morphologies, there is a growing interest in developing approaches for the fabrication of anisotropic hydrogels that exhibit direction-dependent pore shape, microstructure, stiffness, and conductivity. [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] In tissue engineering, aside from biomimicry, anisotropic pore shape and hydrogel structure, in general, are important for cell guidance 22 and differentiation, 23 as well as mass transport of biofactors and nutrients throughout the scaffold. 19,24,25 In bioseparation, control over the shape anisotropy of hydrogel pores may enhance the selectivity of the filtration of biological species and/or minimize the pressure drop across the matrix.…”

Section: Introductionmentioning

confidence: 99%

Composite Hydrogels with Tunable Anisotropic Morphologies and Mechanical Properties

et al. 2016

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Fabrication of anisotropic hydrogels exhibiting direction-dependent structure and properties have attracted great interest in biomimicking, tissue engineering and bioseparation. Herein, we report a single-step freeze casting-based fabrication of structurally and mechanically anisotropic aerogels and hydrogels composed of hydrazone cross-linked poly(oligoethylene glycol methacrylate) (POEGMA) and cellulose nanocrystals (CNCs). We show that by controlling the composition of the CNC/POEGMA dispersion and the freeze casting temperature, aerogels with fibrillar, columnar, or lamellar morphologies can be produced. Small-angle X-ray scattering experiments show that the anisotropy of the structure originates from the alignment of the mesostructures, rather than the CNC building blocks. The composite hydrogels show high structural and mechanical integrity and a strong variation in Young's moduli in orthogonal directions. The controllable morphology and hydrogel anisotropy, coupled with hydrazone cross-linking and biocompatibility of CNCs and POEGMA, provide a versatile platform for the preparation of anisotropic hydrogels.

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“…To respect anisotropic organization of cardiac tissue and promote development of a functional cardiac syncytium, the fibrous structure needs to be aligned. Several studies demonstrated that the presence of aligned surface facilitates the orientation and organization of CMs [36] [37]. Moreover, high surface-to-volume ratio and porosity are indispensable elements for the migration of cells and vascularization.…”

Section: Polymeric Scaffold Characteristics For Cardiac Tissue Enginementioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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Engineering cell alignment in vitro

Cited by 235 publications

References 217 publications

Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Magnetically actuated cell-laden microscale hydrogels for probing strain-induced cell responses in three dimensions

Composite Hydrogels with Tunable Anisotropic Morphologies and Mechanical Properties

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

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