Engineering human cell-based, functionally integrated osteochondral grafts by biological bonding of engineered cartilage tissues to bony scaffolds

Scotti, Celeste; Wirz, Dieter; Wolf, Françine; Schaefer, Dirk J.; Bürgin, Vivienne; Daniels, A. U.; Valderrábano, Víctor; Candrian, Christian; Jakob, Marcel; Martin, Ivan; Barbero, Andrea

doi:10.1016/j.biomaterials.2009.11.110

Cited by 61 publications

(62 citation statements)

References 32 publications

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“…Part of the proposed test protocols has already been applied to in vitro studies [9] and showed reliable results as well. In the cited study, the test was only described cursorily, and the evaluation of the AUC and its comparison to the maximum force were not done.…”

Section: Discussionmentioning

confidence: 92%

90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration

Bürgin

Daniels

Francioli

et al. 2014

International Journal of Tissue Engineering

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One promising treatment of localized osteochondral defects in the knee joint may be the implantation of in vitro tissue-engineered osteochondral constructs. A crucial aspect of this kind of osteochondral construct is the bonding between the bone-scaffold and scaffold-based chondral layer. Here, a 90 ∘ peel off test is proposed as an appropriate method to measure the integration of cartilage to bone in osteochondral constructs for different primary methods of bonding the cartilage scaffold to the bone scaffold, with and without seeded chondroblasts. The method was developed and then tested on tissue-engineered constructs. The force/displacement data obtained allow determination of both the maximum force and the total energy required to separate the two layers. The tests showed good reproducibility and good discrimination between measurements as a function of seeding times. Average maximum peel-off forces varied between 10 mN for fibrin glue only to 575 mN for constructs with cells after four weeks of incubation. Linear regression of the area under the curve (AUC) as a function of maximum force shows a high correlation between the two parameters with R 2 = 0.97. The main limitation of the test is that the data provide only a modest ability to decide how uniform the bond is over the area between the two layers.

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Section: Discussionmentioning

confidence: 92%

90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration

Bürgin

Daniels

Francioli

et al. 2014

International Journal of Tissue Engineering

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“…Scotti et al 99 developed and evaluated osteochondral composites generated by the combination of three clinically compliant materials: Chondro-Gide (containing human chondrocytes), Tutobone, and Tisseel. The clinically relevant osteochondral graft exhibited bonding via biological ECM interactions while exhibiting good stability and processability facilitating a quick implementation within the clinical arena.…”

Section: Stratified/graded Scaffoldsmentioning

confidence: 99%

Recent Progress in Interfacial Tissue Engineering Approaches for Osteochondral Defects

2012

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This review provides a brief synopsis of the anatomy and physiology of the osteochondral interface, scaffold-based and non-scaffold based approaches for engineering both tissues independently as well as recent developments in the manufacture of gradient constructs. Novel manufacturing techniques and nanotechnology will be discussed with potential application in osteochondral interfacial tissue engineering.

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“…Wang et al used polyester/fibrin gel constructs loaded with MSCs and TGF-1 to promote restoration of full-thickness cartilage defects (W. Wang et al, 2010b). Also recently, Scotti et al used fibrin gels to promote cell adhesion to devitalized spongiosa cylinders used for engineering osteochondral grafts (Scotti et al, 2010). In two studies by the laboratory of Lee, MSCs embedded in fibrin glue were able to promote better osseointegration of tendon grafts (Lim et al, 2004;Soon et al, 2007).…”

Section: Fibrinmentioning

confidence: 99%

Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues

Hofmann

García-Fuentes²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

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Engineering human cell-based, functionally integrated osteochondral grafts by biological bonding of engineered cartilage tissues to bony scaffolds

Cited by 61 publications

References 32 publications

90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration

90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration

Recent Progress in Interfacial Tissue Engineering Approaches for Osteochondral Defects

Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues

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