Engineering <i>Propionibacterium acidipropionici</i> for enhanced propionic acid tolerance and fermentation

Zhang, An; Yang, Shang‐Tian

doi:10.1002/bit.22437

Cited by 102 publications

(59 citation statements)

References 31 publications

(39 reference statements)

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“…Fifty microliter of the sample 20 was injected into the 5-mM H 2 SO 4 mobile phase flowing at a rate of 0.6 mL.min -1 . 21 The results shown are the mean of analyses performed in duplicates for all the 22 fermentations. In case of experiments using free cells, the provided data are the mean of two 23 independent replicates.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici

Dishisha

Alvarez

Hatti‐Kaul

2012

Bioresource Technology

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Please cite this article as: Dishisha, T., Alvarez, M.T., Hatti-Kaul, R., Batch-and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici, Bioresource Technology (2012), doi: http://dx.doi.org/10. 1016/j.biortech.2012.05.079 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Analytical Proceduresmentioning

confidence: 99%

Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici

Dishisha

Alvarez

Hatti‐Kaul

2012

Bioresource Technology

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“…During the fermentation of Propionibacterium freudenreichii (P. freudenreichii) for production of food-grade VB12, the extracellular products such as propionic acid and acetic acid are also produced (Hajfarajollah et al 2014). Propionic acid is an important chemical with applications such as mold inhibitor, preservatives in animal feed and human foods, the substrate for synthesis of cellulose fibers, herbicides, perfumes, and pharmaceuticals (Suwannakham et al 2006;Zhang et al 2009). However, the production of these acids has an inhibiting effect on the cell growth and thus on the production of VB12 (Wang et al 2012;Miyano and Shimizu 2000).…”

Section: Introductionmentioning

confidence: 99%

Vitamin B12 biosynthesis over waste frying sunflower oil as a cost effective and renewable substrate

et al. 2014

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Statistical experimental designs were used to develop a medium based on waste frying sunflower oil (WFO) and other nutrient sources for production of vitamin B 12 (VB12) by Propionibacterium freudenreichii subsp. freudenreichii PTCC 1674. The production of acetic acid and propionic acid were also evaluated using the same microorganism. The amount of WFO in the media was initially optimized. The amount of 4 % w/v of oil found to be an appropriate amount for production of VB12. A Plackett Burman design was then employed to identify nutrients that have significant effect on the production of VB12 in the WFO media. Dimethylbenzimidazolyl (DMB), cobalt chloride, ferrous sulfate, and calcium chloride were the most important compounds. The level optimization of nutrients as the significant factors was finally performed using response surface methodology based on a central composite design. The model predicted that a medium containing 35.56 mg/L DMB, 14.69 mg/L CoCl 2 .6H 2 O, 5.82 mg/L FeSO 4 .7H 2 O, and 11.41 mg/L CaCl 2 .2H 2 O gives the maximum VB12 production of 2.60 mg/L. The optimized medium provides a final concentration of vitamin 170 % higher than that by the original medium. This study offers valuable insights on a costeffective carbon source for industrial production of food-grade VB12.

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“…The reported species used for PA production include P. freudenreichii (6), P. acidipropionici (5,(7)(8)(9)(10)(11)(12)(13)(14), and P. thoenii (15). Among those, P. acidipropionici is the main producer of PA, with a maximal yield of 97 g/liter from a 3-month fed-batch culture of a PA-tolerant P. acidipropionici mutant in a fibrous bed bioreactor (31). Figure 1 shows the time profiles of PA production by batch culture of P. jensenii ATCC 4868.…”

Section: Resultsmentioning

confidence: 99%

Development of a Propionibacterium-Escherichia coli Shuttle Vector for Metabolic Engineering of Propionibacterium jensenii, an Efficient Producer of Propionic Acid

Zhuge

Liu

Shin

et al. 2013

Appl Environ Microbiol

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dPropionic acid (PA) is an important chemical building block and is widely applied for organic synthesis, food, feedstuff, and pharmaceuticals. To date, the strains that can efficiently produce PA have included Propionibacterium thoenii, P. freudenreichii, and P. acidipropionici. In this report, we show that P. jensenii ATCC 4868 is also able to produce PA in much higher yields than the previously reported strains. To further improve the production capacity, a P. jensenii-Escherichia coli shuttle vector was developed for the metabolic engineering of P. jensenii. Specifically, a 6.9-kb endogenous plasmid, pZGX01, was isolated from P. acidipropionici ATCC 4875 and sequenced. Since the sequencing analysis indicated that pZGX01 could encode 11 proteins, the transcriptional levels of the corresponding genes were also investigated. Then, a P. jensenii-Escherichia coli shuttle vector was constructed using the pZGX01 plasmid, the E. coli pUC18 plasmid, and a chloramphenicol resistance gene. Interestingly, not only could the developed shuttle vector be transformed into P. jensenii ATCC 4868 and 4870, but it also could be transformed into freudenreichii ATCC 6207 subspecies of P. freudenreichii. Finally, the glycerol dehydrogenase gene (gldA) from Klebsiella pneumoniae was expressed in P. jensenii ATCC 4868 with the constructed shuttle vector. In a 3-liter batch culture, the PA production by the engineered P. jensenii ATCC 4868 strain reached 28.23 ؎ 1.0 g/liter, which was 26.07% higher than that produced by the wild-type strain (22.06 ؎ 1.2 g/liter). This result indicated that the constructed vector can be used a useful tool for metabolic engineering of P. jensenii.

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Engineering Propionibacterium acidipropionici for enhanced propionic acid tolerance and fermentation

Cited by 102 publications

References 31 publications

Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici

Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici

Vitamin B12 biosynthesis over waste frying sunflower oil as a cost effective and renewable substrate

Development of a Propionibacterium-Escherichia coli Shuttle Vector for Metabolic Engineering of Propionibacterium jensenii, an Efficient Producer of Propionic Acid

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