Engineering injectable vascularized tissues from the bottom-up: Dynamics of in-gel extra-spheroid dermal tissue assembly

Feijão, Tália; Neves, Mariana I.; Sousa, Aureliana; Torres, Ana Luísa; Bidarra, Sílvia J.; Orge, Iasmim Diniz; Carvalho, Daniel J; Barrias, Cristina C.

doi:10.1016/j.biomaterials.2021.121222

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…Endothelial cadherin (VE‐cadherin, gene name: CDH5) is a homotypic adhesion protein that is selectively expressed by endothelial cells and can form tight blood vessels and regulate the connection of endothelial cells. [ 42 ] Platelet‐endothelial cell adhesion molecule‐1 (PECAM1) is a vascular cell adhesion molecule that mediates various important cell‐cell interactions. [ 43 ] Additionally, VEGF‐A is a proangiogenic factor that can regulate angiogenesis and maintains vascular stability under embryonic development or physiological and pathological conditions.…”

Section: Resultsmentioning

confidence: 99%

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Feng

Liu²,

Chen

et al. 2023

Adv Healthcare Materials

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Post‐injury infection and wound healing are recurrent daily life problems. Therefore, the necessity of developing a biomaterial with antibacterial and wound‐healing properties is paramount. Based on the special porous structure of hydrogel, this work modifies recombinant collagen and quaternary ammonium chitosan and fused them with silver nanoparticles (Ag@mental‐organic framework (Ag@MOF)) with antibacterial properties, and asiaticoside‐loaded liposomes (Lip@AS) with anti‐inflammatory/vascularization effects to form the rColMA/QCSG/LIP@AS/Ag@MOF (RQLAg) hydrogel. The prepared hydrogel possesses good sustainable release capabilities of Ag+ and AS and exhibits concentration‐dependent swelling properties, pore size, and compressive strength. Cellular experiments show that the hydrogel exhibit good cell compatibility and promote cell migration, angiogenesis, and M1 macrophage polarization. Additionally, the hydrogels exhibit excellent antibacterial activity against Escherichia coli and Staphylococcus aureus in vitro. In vivo, Sprague Dawley rats burn‐wound infection model showed that the RQLAg hydrogel could efficiently promote wound healing and has stronger healing promoting abilities than those of Aquacel Ag. In summary, the RQLAg hydrogel is expected to be an excellent material for accelerating open wound healing and preventing bacterial infections.

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Section: Resultsmentioning

confidence: 99%

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Feng

Liu²,

Chen

et al. 2023

Adv Healthcare Materials

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“…The membrane of chick embryo provides a non-innervated and rapidly growing vascular bed, which can serve as a blood supply for engineered tissues and, therefore, be a useful model for testing prevascularization strategies [ 55 ]. In particular, its use has been reported in approaches ranging from cell sheets [ 56 ] to spheroids [ 57 ]. Herein, prevascularized collagen sponges were implanted onto the CAM and collected after 4 days.…”

Section: Discussionmentioning

confidence: 99%

“…A CAM assay was performed as previously described [ 57 , 63 ]. White fertilized chicken eggs were incubated at 37 °C in a temperature incubator (Termaks KB8000, Bergen, Norway) for 3 days.…”

Section: Methodsmentioning

confidence: 99%

Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue

et al. 2022

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The successful integration of transplanted three-dimensional tissue engineering (TE) constructs depends greatly on their rapid vascularization. Therefore, it is essential to address this vascularization issue in the initial design of constructs for perfused tissues. Two of the most important variables in this regard are scaffold composition and cell sourcing. Collagens with marine origins overcome some issues associated with mammal-derived collagen while maintaining their advantages in terms of biocompatibility. Concurrently, the freshly isolated stromal vascular fraction (SVF) of adipose tissue has been proposed as an advantageous cell fraction for vascularization purposes due to its highly angiogenic properties, allowing extrinsic angiogenic growth factor-free vascularization strategies for TE applications. In this study, we aimed at understanding whether marine collagen 3D matrices could support cryopreserved human SVF in maintaining intrinsic angiogenic properties observed for fresh SVF. For this, cryopreserved human SVF was seeded on blue shark collagen sponges and cultured up to 7 days in a basal medium. The secretome profile of several angiogenesis-related factors was studied throughout culture times and correlated with the expression pattern of CD31 and CD146, which showed the formation of a prevascular network. Upon in ovo implantation, increased vessel recruitment was observed in prevascularized sponges when compared with sponges without SVF cells. Immunohistochemistry for CD31 demonstrated the improved integration of prevascularized sponges within chick chorioalantoic membrane (CAM) tissues, while in situ hybridization showed human cells lining blood vessels. These results demonstrate the potential of using cryopreserved SVF combined with marine collagen as a streamlined approach to improve the vascularization of TE constructs.

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“…MoA allows for the extra-spheroid assembly of intricately vascularized tissues. Feijo et al reported a technique for the production of vascularized dermal macro-tissue from fibroblast/endothelial progenitor spheroids [141]. Spheroids embedded in fibrin provided a 3D milieu for extra-spheroid development.…”

Section: Pre-vascularized Moa For Advanced Tissue Modelingmentioning

confidence: 99%

Engineering vascularized organotypic tissues via module assembly

Zhou,

Liu,

Guo

et al. 2023

Int. J. Extrem. Manuf.

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Adequate vascularization is a critical determinant for the successful construction and clinical implementation of complex organotypic tissue models. Currently, low cell and vessel density and insufficient vascular maturation make vascularized organotypic tissue construction difficult, greatly limiting its use in tissue engineering and regenerative medicine. To address these limitations, recent studies have adopted pre-vascularized microtissue assembly for the rapid generation of functional tissue analogs with dense vascular networks and high cell density. In this article, we summarize the development of module assembly-based vascularized organotypic tissue construction and its application in tissue repair and regeneration, organ-scale tissue biomanufacturing, and advanced tissue modeling.

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Engineering injectable vascularized tissues from the bottom-up: Dynamics of in-gel extra-spheroid dermal tissue assembly

Cited by 12 publications

References 35 publications

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue

Engineering vascularized organotypic tissues via module assembly

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