Engineering Lactococcus lactis for the production of unusual anthocyanins using tea as substrate

Solopova, Ana; Tilburg, Amanda Y van; Foito, Alexandre; Allwood, J. William; Stewart, Derek; Kulakauskas, Saulius; Kuipers, Oscar P.

doi:10.1016/j.ymben.2019.04.002

Cited by 32 publications

(16 citation statements)

References 48 publications

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“…Nowadays, many compounds, including phenolic acids, can be produced using engineered microorganisms. These microbial cell factories can be cost-effective and environmentally friendly alternatives, delivering the yield and purity of compounds that are higher than those extracted from plants [ 30 , 31 ]. Notably, phenolic acids produced in plants are usually found in conjugated form, often covalently bound to carbohydrates [ 32 ], whereas the same phenolic acids produced by microorganisms are obtained as free compounds.…”

Section: Introductionmentioning

confidence: 99%

Advances and Prospects of Phenolic Acids Production, Biorefinery and Analysis

et al. 2020

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Biotechnological production of phenolic acids is attracting increased interest due to their superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As secondary metabolites, primarily found in plants and fungi, they are effective free radical scavengers due to the phenolic group available in their structure. Therefore, phenolic acids are widely utilised by pharmaceutical, food, cosmetic, and chemical industries. A demand for phenolic acids is mostly satisfied by utilising chemically synthesised compounds, with only a low quantity obtained from natural sources. As an alternative to chemical synthesis, environmentally friendly bio-based technologies are necessary for development in large-scale production. One of the most promising sustainable technologies is the utilisation of microbial cell factories for biosynthesis of phenolic acids. In this paper, we perform a systematic comparison of the best known natural sources of phenolic acids. The advances and prospects in the development of microbial cell factories for biosynthesis of these bioactive compounds are discussed in more detail. A special consideration is given to the modern production methods and analytics of phenolic acids.

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Section: Introductionmentioning

confidence: 99%

Advances and Prospects of Phenolic Acids Production, Biorefinery and Analysis

et al. 2020

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“…Metabolic engineering of pathway enzymes for microbial production of flavonoids proves to be a promising way of supplying flavonoid compounds for industrial applications. It also allows the biosynthesis of rare flavonoid compounds, such as pyranoanthocyanins (Akdemir et al., 2019; Solopova et al., 2019). With the understanding of pathway enzymes, researchers have successfully reconstituted the biosynthetic pathways of various flavonoid compounds in E. coli , S. cerevisiae , S. venezuelae , and C. glutamicum .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Pathway enzyme engineering for flavonoid production in recombinant microbes

Zha

Gong

et al. 2019

Metabolic Engineering Communications

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Metabolic engineering of microbial strains for the production of flavonoids of industrial interest has attracted great attention due to its promising advantages over traditional extraction approaches, such as independence of plantation, facile downstream separation, and ease of process and quality control. However, most of the constructed microbial production systems suffer from low production titers, low yields and low productivities, restricting their commercial applications. One important reason of the inefficient production is that the expression conditions and the detailed functions of the flavonoid pathway enzymes are not well understood. In this review, we have collected the biochemical properties, structural details, and genetic information of the enzymes in the flavonoid biosynthetic pathway as a guide for the expression and analysis of these enzymes in microbial systems. Additionally, we have summarized the engineering approaches used in improving the performances of these enzymes in recombinant microorganisms. Major challenges and future directions on the flavonoid pathway are also discussed.

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“…Many studies have focused on the enhancement of flavonoid production in bacteria [ 27 , 28 ], yeast [ 29 ], and plants [ 21 ]. Hairy roots from various plant species have been developed for the production of phenolic compounds because of their ability to produce large amounts of metabolites and their high growth rates [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots Overexpressing ZmLC

Park

Yeo

et al. 2021

IJMS

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Fagopyrum tataricum ‘Hokkai T10′ is a buckwheat cultivar capable of producing large amounts of phenolic compounds, including flavonoids (anthocyanins), phenolic acids, and catechin, which have antioxidant, anticancer, and anti-inflammatory properties. In the present study, we revealed that the maize transcription factor Lc increased the accumulation of phenolic compounds, including sinapic acid, 4-hydroxybenzonate, t-cinnamic acid, and rutin, in Hokkai T10 hairy roots cultured under long-photoperiod (16 h light and 8 h dark) conditions. The transcription factor upregulated phenylpropanoid and flavonoid biosynthesis pathway genes, yielding total phenolic contents reaching 27.0 ± 3.30 mg g−1 dry weight, 163% greater than the total flavonoid content produced by a GUS-overexpressing line (control). In contrast, when cultured under continuous darkness, the phenolic accumulation was not significantly different between the ZmLC-overexpressing hairy roots and the control. These findings suggest that the transcription factor (ZmLC) activity may be light-responsive in the ZmLC-overexpressing hairy roots of F. tataricum, triggering activation of the phenylpropanoid and flavonoid biosynthesis pathways. Further studies are required on the optimization of light intensity in ZmLC-overexpressing hairy roots of F. tataricum to enhance the production of phenolic compounds.

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Engineering Lactococcus lactis for the production of unusual anthocyanins using tea as substrate

Cited by 32 publications

References 48 publications

Advances and Prospects of Phenolic Acids Production, Biorefinery and Analysis

Advances and Prospects of Phenolic Acids Production, Biorefinery and Analysis

Pathway enzyme engineering for flavonoid production in recombinant microbes

Effect of Light and Dark on the Phenolic Compound Accumulation in Tartary Buckwheat Hairy Roots Overexpressing ZmLC

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