Engineering microenvironment for human cardiac tissue assembly in heart-on-a-chip platform

Zhao, Yong; Rafatian, Naimeh; Wang, Erika Y.; Feric, Nicole; Lai, Benjamin; Knee‐Walden, Ericka J.; Backx, Peter H.; Radišić, Milica

doi:10.1016/j.matbio.2019.04.001

Cited by 80 publications

(73 citation statements)

References 51 publications

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“…On the other hand, the development of bioreactor platforms has already enabled the production of engineered heart tissues in an automated, standardized, and repeatable way. Bioreactors allow for strict control of the microenvironment of the cardiomyocytes: Zhao et al recently built a heart-on-chip system in which small tissue strips could be formed and electrically conditioned, and the tissue contractions could be monitored [ 173 ]. They obtained well-formed tissues showing a positive force–frequency relationship and post-rest potentiation.…”

Section: Limitations and Perspectives Of Cardiac Organoidsmentioning

confidence: 99%

Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

et al. 2021

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Cardiac tissue engineering aims at creating contractile structures that can optimally reproduce the features of human cardiac tissue. These constructs are becoming valuable tools to model some of the cardiac functions, to set preclinical platforms for drug testing, or to alternatively be used as therapies for cardiac repair approaches. Most of the recent developments in cardiac tissue engineering have been made possible by important advances regarding the efficient generation of cardiac cells from pluripotent stem cells and the use of novel biomaterials and microfabrication methods. Different combinations of cells, biomaterials, scaffolds, and geometries are however possible, which results in different types of structures with gradual complexities and abilities to mimic the native cardiac tissue. Here, we intend to cover key aspects of tissue engineering applied to cardiology and the consequent development of cardiac organoids. This review presents various facets of the construction of human cardiac 3D constructs, from the choice of the components to their patterning, the final geometry of generated tissues, and the subsequent readouts and applications to model and treat cardiac diseases.

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Section: Limitations and Perspectives Of Cardiac Organoidsmentioning

confidence: 99%

Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

et al. 2021

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“…It is also worthwhile to note that broblasts act as a activator of contractility and ring of CMs by lowering membrane potentials. 43,68 Thus, our ndings in the enhanced synchronization of LBL mCMTs could be explained by the fact that CMs were interconnected each other without the physical intercalation of CFs, but with their activating effects in proximity. This is also supported by our analysis that the cardiac maturation marker, MLC2v and cTNI, was upregulated in a RNA level ( Fig.…”

Section: Discussionmentioning

confidence: 84%

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

et al. 2020

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“…These operating volumes will not support high-throughput analysis such as microfluidics ( Tumarkin et al, 2011 ). Micro-bioreactors, and those operating in the milliliter scale, have been suitable platforms for drug-screening, stem cell differentiation protocols or organ-on-a-chip devices as they enable expensive processes to be conducted in a cost-effective way, a consequence of the small operating volumes, otherwise prohibitive at larger scales ( Kane et al, 2019 ; Rajan et al, 2020 ; Zhao et al, 2020 ). However, the sFBB system offers an appropriate scale, operating in perfusion mode, for the intermediate stage of testing methods in a more physiologically relevant volume or expanding optimized protocols.…”

Section: Discussionmentioning

confidence: 99%

Small-Scale Fluidized Bed Bioreactor for Long-Term Dynamic Culture of 3D Cell Constructs and in vitro Testing

Silva

Erro

Awan

et al. 2020

Front. Bioeng. Biotechnol.

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With the increasing interest in three-dimensional (3D) cell constructs that better represent native tissues, comes the need to also invest in devices, i.e., bioreactors, that provide a controlled dynamic environment similar to the perfusion mechanism observed in vivo. Here a laboratory-scale fluidized bed bioreactor (sFBB) was designed for hydrogel (i.e., alginate) encapsulated cells to generate a dynamic culture system that produced a homogenous milieu and host substantial biomass for long-term evolution of tissue-like structures and "per cell" performance analysis. The bioreactor design, conceptualized through scale-down empirical similarity rules, was initially validated through computational fluid dynamics analysis for the distributor capacity of homogenously dispersing the flow with an average fluid velocity of 4.596 × 10 −4 m/s. Experimental tests then demonstrated a consistent fluidization of hydrogel spheres, while maintaining shape and integrity (606.9 ± 99.3 µm diameter and 0.96 shape factor). It also induced mass transfer in and out of the hydrogel at a faster rate than static conditions. Finally, the sFBB sustained culture of alginate encapsulated hepatoblastoma cells for 12 days promoting proliferation into highly viable (>97%) cell spheroids at a high final density of 27.3 ± 0.78 million cells/mL beads. This was reproducible across multiple units set up in parallel and operating simultaneously. The sFBB prototype constitutes a simple and robust tool to generate 3D cell constructs, expandable into a multi-unit setup for simultaneous observations and for future development and biological evaluation of in vitro tissue models and their responses to different agents, increasing the complexity and speed of R&D processes.

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Engineering microenvironment for human cardiac tissue assembly in heart-on-a-chip platform

Cited by 80 publications

References 51 publications

Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

Small-Scale Fluidized Bed Bioreactor for Long-Term Dynamic Culture of 3D Cell Constructs and in vitro Testing

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