Engineering next-generation bioinks with nanoparticles: moving from reinforcement fillers to multifunctional nanoelements

Bakht, Syeda Mahwish; Pardo, Alberto; Gomez‐Florit, Manuel; Reis, Rui L.; Domingues, Rui M. A.; Gomes, Manuela E.

doi:10.1039/d1tb00717c

Cited by 32 publications

(31 citation statements)

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“…Several studies have shown that bioink printability is governed by bioink composition and printing parameters. , To enhance the printability and shear-thinning properties of hydrogels in general, incorporation of different additives such as nanoparticles and 2D materials has been employed and has, so far, demonstrated excellent results. − For GelMA hydrogels, in particular, efforts have been devoted to high concentrations rather than low ones, although low concentrations produce better cell-laden constructs …”

Section: Introductionmentioning

confidence: 99%

Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

Boularaoui

Shanti

Lanotte

et al. 2021

ACS Biomater. Sci. Eng.

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There is a growing need to develop novel well-characterized biological inks (bioinks) that are customizable for three-dimensional (3D) bioprinting of specific tissue types. Gelatin methacryloyl (GelMA) is one such candidate bioink due to its biocompatibility and tunable mechanical properties. Currently, only low-concentration GelMA hydrogels (≤5% w/v) are suitable as cell-laden bioinks, allowing high cell viability, elongation, and migration. Yet, they offer poor printability. Herein, we optimize GelMA bioinks in terms of concentration and cross-linking time for improved skeletal muscle C2C12 cell spreading in 3D, and we augment these by adding gold nanoparticles (AuNPs) or a two-dimensional (2D) transition metal carbide (MXene nanosheets) for enhanced printability and biological properties. AuNP and MXene addition endowed GelMA with increased conductivity (up to 0.8 ± 0.07 and 0.9 ± 0.12 S/m, respectively, compared to 0.3 ± 0.06 S/m for pure GelMA). Furthermore, it resulted in an improvement of rheological properties and printability, specifically at 10 °C. Improvements in electrical and rheological properties led to enhanced differentiation of encapsulated myoblasts and allowed for printing highly viable (97%) stable constructs. Taken together, these results constitute a significant step toward fabrication of 3D conductive tissue constructs with physiological relevance.

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Section: Introductionmentioning

confidence: 99%

Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

Boularaoui

Shanti

Lanotte

et al. 2021

ACS Biomater. Sci. Eng.

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“…For example, biofabrication is an emerging and rapidly growing research field in which additive manufacturing has been merged with tissue engineering to generate hierarchical tissue-like and personalized constructs. On the basis of 3D bioprinting technology, bioinks combining high-resolution printability with cytocompatibility have been developed as one ideal scaffold material for clinical translation, which will continue to be an active participant in the process of bone regeneration, not only as cells and molecular carriers, but also playing an important role in controlling delivery efficiency and delivery rate, thus making bioprinting hydrogel systems appealing alternatives for tissue engineering and drug delivery purposes, among others [ 93 , 94 , 95 , 96 ]. Therefore, it is of great significance to construct osteoblast and osteoclast co-culture models based on 3D printing techniques for promoting bone regeneration and studying the interaction between cells.…”

Section: Discussionmentioning

confidence: 99%

Advances of Stimulus-Responsive Hydrogels for Bone Defects Repair in Tissue Engineering

Chang

Wang

Liu

et al. 2022

Gels

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Bone defects, as one of the most urgent problems in the orthopedic clinic, have attracted much attention from the biomedical community and society. Hydrogels have been widely used in the biomedical field for tissue engineering research because of their excellent hydrophilicity, biocompatibility, and degradability. Stimulus-responsive hydrogels, as a new type of smart biomaterial, have more advantages in sensing external physical (light, temperature, pressure, electric field, magnetic field, etc.), chemical (pH, redox reaction, ions, etc.), biochemical (glucose, enzymes, etc.) and other different stimuli. They can respond to stimuli such as the characteristics of the 3D shape and solid–liquid phase state, and exhibit special properties (injection ability, self-repair, shape memory, etc.), thus becoming an ideal material to provide cell adhesion, proliferation, and differentiation, and achieve precise bone defect repair. This review is focused on the classification, design concepts, and research progress of stimulus-responsive hydrogels based on different types of external environmental stimuli, aiming at introducing new ideas and methods for repairing complex bone defects.

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“…These molecules enhance a more biomimetic microenvironment that simulates the natural signaling and repair mechanisms thus influencing tissue formation and cellular functions (45). Bioactive inorganic fillers and nanomaterials such as graphene, graphene oxide, carbon nanotubes, calcium phosphates, bioactive glasses, silica nanoparticles, and nanoclays have also been used in hydrogel bioinks to improve printability, cell viability, and mechanical properties (155)(156)(157). These fillers could also be doped with drugs or biologically active ions to induce specific responses or act as crosslinkers (155).…”

Section: Scaffoldsmentioning

confidence: 99%

“…These fillers could also be doped with drugs or biologically active ions to induce specific responses or act as crosslinkers (155). We refer the reader to these comprehensive reviews on the topic (155)(156)(157)(158). As an example of functionalization, Modaresifara et al developed a gelatin methacryloyl (GelMA) hydrogel that incorporated chitosan nanoparticles to promote growth factor delivery (158).…”

Section: Scaffoldsmentioning

confidence: 99%

3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations

Marei

Samaan

Al-Quradaghi

et al. 2022

Front. Cardiovasc. Med.

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Despite the efforts devoted to drug discovery and development, the number of new drug approvals have been decreasing. Specifically, cardiovascular developments have been showing amongst the lowest levels of approvals. In addition, concerns over the adverse effects of drugs to the cardiovascular system have been increasing and resulting in failure at the preclinical level as well as withdrawal of drugs post-marketing. Besides factors such as the increased cost of clinical trials and increases in the requirements and the complexity of the regulatory processes, there is also a gap between the currently existing pre-clinical screening methods and the clinical studies in humans. This gap is mainly caused by the lack of complexity in the currently used 2D cell culture-based screening systems, which do not accurately reflect human physiological conditions. Cell-based drug screening is widely accepted and extensively used and can provide an initial indication of the drugs' therapeutic efficacy and potential cytotoxicity. However, in vitro cell-based evaluation could in many instances provide contradictory findings to the in vivo testing in animal models and clinical trials. This drawback is related to the failure of these 2D cell culture systems to recapitulate the human physiological microenvironment in which the cells reside. In the body, cells reside within a complex physiological setting, where they interact with and respond to neighboring cells, extracellular matrix, mechanical stress, blood shear stress, and many other factors. These factors in sum affect the cellular response and the specific pathways that regulate variable vital functions such as proliferation, apoptosis, and differentiation. Although pre-clinical in vivo animal models provide this level of complexity, cross species differences can also cause contradictory results from that seen when the drug enters clinical trials. Thus, there is a need to better mimic human physiological conditions in pre-clinical studies to improve the efficiency of drug screening. A novel approach is to develop 3D tissue engineered miniaturized constructs in vitro that are based on human cells. In this review, we discuss the factors that should be considered to produce a successful vascular construct that is derived from human cells and is both reliable and reproducible.

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Engineering next-generation bioinks with nanoparticles: moving from reinforcement fillers to multifunctional nanoelements

Abstract: The application of additive manufacturing in the biomedical field has become a hot topic in the last decade owing to its potential to provide personalized solutions for patients. Different bioinks...

Cited by 32 publications

References 135 publications

Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

Advances of Stimulus-Responsive Hydrogels for Bone Defects Repair in Tissue Engineering

3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations

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